scholarly journals The Role of Protein Engineering in Biomedical Applications of Mammalian Synthetic Biology

Small ◽  
2019 ◽  
Vol 16 (27) ◽  
pp. 1903093 ◽  
Author(s):  
Daniel Bojar ◽  
Martin Fussenegger
Keyword(s):  
Synthetic Biology ◽  
Protein Engineering ◽  
Biomedical Applications

The imminent role of protein engineering in synthetic biology

2012 ◽  
Vol 30 (3) ◽  
pp. 541-549 ◽  
Author(s):  
Jee Loon Foo ◽  
Chi Bun Ching ◽  
Matthew Wook Chang ◽  
Susanna Su Jan Leong
Keyword(s):  
Synthetic Biology ◽  
Protein Engineering

Microbial Biofilms and Biotechnology – Some Perceptions

2020 ◽  
Vol 09 ◽  
Author(s):  
Subba Rao Toleti
Keyword(s):  
Tissue Culture ◽  
Synthetic Biology ◽  
Industrial Effluents ◽  
Aquatic Environments ◽  
Biochemical Processes ◽  
Microbial Biofilms ◽  
Biofilm Bacteria ◽  
Prophylactic Vaccines ◽  
Design Construction

: The review is an attempt to introduce the readers in brief about biofilms and their implications as well as some new perceptions in biotechnology. Biofilms are adherent microbial communities, which are developed on submerged surfaces in aquatic environments. Biofilms play a significant role in exopolymer production, material deterioration and also cause harmful infections. Further, the role of corrosion causing biofilm bacteria in deterioration of different materials, microbial biofilms and their enzymatic processes in reducing the toxicity of pollutants in industrial effluents are elaborated, along with clean technologies for wastewater treatment. Biotechnology is defined as any technological application that uses biological systems to synthesize or modify products or processes. The applications include biochemical processes, medical care, cell and tissue culture as well as synthetic biology and others. Synthetic biology details about the design, construction of new biological components and systems for useful purposes. Finally, to overcome the limitations that are inherent to the use of cellular host’s, cell-free systems as critical platforms for synthetic biology applications. This mini-review also mentions about new diagnostic products based on enzymes, monoclonal antibodies and engineered proteins as well as novel prophylactic vaccines.

scholarly journals Protein and Polysaccharide-Based Electroactive and Conductive Materials for Biomedical Applications

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4499
Author(s):  
Xiao Hu ◽  
Samuel Ricci ◽  
Sebastian Naranjo ◽  
Zachary Hill ◽  
Peter Gawason
Keyword(s):  
Cell Biology ◽  
Biomedical Applications ◽  
Human Life ◽  
Electrically Conductive ◽  
Conductive Materials ◽  
Production Strategies ◽  
Integral Role ◽  
Material Sciences ◽  
Novel Drug

Electrically responsive biomaterials are an important and emerging technology in the fields of biomedical and material sciences. A great deal of research explores the integral role of electrical conduction in normal and diseased cell biology, and material scientists are focusing an even greater amount of attention on natural and hybrid materials as sources of biomaterials which can mimic the properties of cells. This review establishes a summary of those efforts for the latter group, detailing the current materials, theories, methods, and applications of electrically conductive biomaterials fabricated from protein polymers and polysaccharides. These materials can be used to improve human life through novel drug delivery, tissue regeneration, and biosensing technologies. The immediate goal of this review is to establish fabrication methods for protein and polysaccharide-based materials that are biocompatible and feature modular electrical properties. Ideally, these materials will be inexpensive to make with salable production strategies, in addition to being both renewable and biocompatible.

scholarly journals Effect of tungsten disulfide nanotubes on crystallization of polylactide under uniaxial deformation and annealing

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Fausta Loffredo ◽  
Loredana Tammaro ◽  
Tiziana Di Luccio ◽  
Carmela Borriello ◽  
Fulvia Villani ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Structural Evolution ◽  
Tungsten Disulfide ◽  
Fine Tuning ◽  
Nanocomposite Films ◽  
Uniaxial Deformation ◽  
Scanning Calorimetry ◽  
X Ray ◽  
X Ray Scattering

AbstractTungsten disulfide (WS2) nanotubes (NTs) are examined here as a filler for polylactide (PLA) for their ability to accelerate PLA crystallization and for their promising biocompatibility in relevant to biomedical applications of PLA-WS2 nanocomposites. In this work, we have studied the structural and thermal properties of PLA-WS2 nanocomposite films varying the concentration of WS2 NTs from 0 (neat PLA) to 0.6 wt%. The films were uniaxially drawn at 90 °C and annealed at the same temperature for 3 and 10 min. Using wide angle x-ray scattering, Raman spectroscopy and differential scanning calorimetry, we probed the effects of WS2 NT addition on the structure of the PLA films at various stages of processing (unstretched, stretching, annealing). We found that 0.6 wt% of WS2 induces the same level of crystallinity in as stretched PLA-WS2 as annealing in neat PLA for 10 min. These data provide useful insights into the role of WS2 NTs on the structural evolution of PLA-WS2 composites under uniaxial deformation, and extend their applicability to situations where fine tuning of PLA crystallinity is desirable.

Role of polymers in the design of 3D carbon nanotube-based scaffolds for biomedical applications

2014 ◽  
Vol 39 (7) ◽  
pp. 1448-1471 ◽  
Author(s):  
María C. Serrano ◽  
María C. Gutiérrez ◽  
Francisco del Monte
Keyword(s):  
Carbon Nanotube ◽  
Biomedical Applications

scholarly journals Protein Engineering Approaches to Enhance Fungal Laccase Production in S. cerevisiae

2021 ◽  
Vol 22 (3) ◽  
pp. 1157
Author(s):  
Pablo Aza ◽  
Felipe de Salas ◽  
Gonzalo Molpeceres ◽  
David Rodríguez-Escribano ◽  
Iñigo de la Fuente ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Directed Evolution ◽  
Signal Peptide ◽  
Laccase Activity ◽  
Laccase Production ◽  
Wide Range ◽  
Mating Factor ◽  
Conserved Amino Acids ◽  
Basidiomycete Fungi

Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is a preferred host for engineering fungal laccases. To assist the difficult secretion of active enzymes by yeast, the native signal peptide is usually replaced by the preproleader of S. cerevisiae alfa mating factor (MFα1). However, in most cases, only basal enzyme levels are obtained. During directed evolution in S. cerevisiae of laccases fused to the α-factor preproleader, we demonstrated that mutations accumulated in the signal peptide notably raised enzyme secretion. Here we describe different protein engineering approaches carried out to enhance the laccase activity detected in the liquid extracts of S. cerevisiae cultures. We demonstrate the improved secretion of native and engineered laccases by using the fittest mutated α-factor preproleader obtained through successive laccase evolution campaigns in our lab. Special attention is also paid to the role of protein N-glycosylation in laccase production and properties, and to the introduction of conserved amino acids through consensus design enabling the expression of certain laccases otherwise not produced by the yeast. Finally, we revise the contribution of mutations accumulated in laccase coding sequence (CDS) during previous directed evolution campaigns that facilitate enzyme production.

scholarly journals Will the Bacteria Survive in the CeO2 Nanozyme-H2O2 System?

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3747
Author(s):  
Weisheng Zhu ◽  
Luyao Wang ◽  
Qisi Li ◽  
Lizhi Jiao ◽  
Xiaokan Yu ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Biomedical Applications ◽  
Antibacterial Effect ◽  
Ros Production ◽  
Ros Scavenging ◽  
Ros Scavenger ◽  
Oxide Nanoparticle ◽  
H2o2 System ◽  
Bacterial Disinfection

As one of the nanostructures with enzyme-like activity, nanozymes have recently attracted extensive attention for their biomedical applications, especially for bacterial disinfection treatment. Nanozymes with high peroxidase activity are considered to be excellent candidates for building bacterial disinfection systems (nanozyme-H2O2), in which the nanozyme will promote the generation of ROS to kill bacteria based on the decomposition of H2O2. According to this criterion, a cerium oxide nanoparticle (Nanoceria, CeO2, a classical nanozyme with high peroxidase activity)-based nanozyme-H2O2 system would be very efficient for bacterial disinfection. However, CeO2 is a nanozyme with multiple enzyme-like activities. In addition to high peroxidase activity, CeO2 nanozymes also possess high superoxide dismutase activity and antioxidant activity, which can act as a ROS scavenger. Considering the fact that CeO2 nanozymes have both the activity to promote ROS production and the opposite activity for ROS scavenging, it is worth exploring which activity will play the dominating role in the CeO2-H2O2 system, as well as whether it will protect bacteria or produce an antibacterial effect. In this work, we focused on this discussion to unveil the role of CeO2 in the CeO2-H2O2 system, so that it can provide valuable knowledge for the design of a nanozyme-H2O2-based antibacterial system.

scholarly journals In silico study of PEI-PEG-squalene-dsDNA polyplex formation: The delicate role of PEG length to the binding of PEI to DNA

2021 ◽  
Author(s):  
Tudor Vasiliu ◽  
Bogdan Florin Florin Craciun ◽  
Andrei Neamtu ◽  
Lilia Clima ◽  
Dragos Lucian Isac ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Gene Delivery ◽  
In Silico ◽  
Biomedical Applications ◽  
Experimental Studies ◽  
Biomedical Devices ◽  
In Silico Study ◽  
Or Gene

The biocompatible hydrophilic polyethylene glycol (PEG) is widely used in biomedical applications, such as drug or gene delivery, tissue engineering or as antifouling in biomedical devices. Experimental studies have shown...

Future trends in carbon nanotubes: Process towards a viable biomedical solution

2021 ◽  
Vol 06 ◽  
Author(s):  
Raja Murugesan ◽  
Sureshkumar Raman ◽  
Arun Radhakrishnan
Keyword(s):  
Carbon Nanotubes ◽  
Carbon Nanotube ◽  
Biomedical Applications ◽  
Scale Up ◽  
Diagnostic Techniques ◽  
Nano Composite ◽  
Methods Of Synthesis ◽  
In Trans ◽  
Functionalization Of Carbon Nanotubes

Background: Recently, Nanomaterials based nano-composite materials play the role of various field. Especially, Carbon nanotube based materials are involved in the bio-medical applications.Since, their exclusive and exciting property, researchers worldwide have extensively involved in trans-modulating the carbon nanotubes into a viable medico-friendly system. Objective: These active researches paved the path towards targeted drug delivery, diagnostic techniques, and bio-analytical applications. Despite these exciting properties, which accomplish the probable for biomedical applications, they hold Biosafety issues. Methods: This broad-spectrum review has discussed different aspects of carbon nanotubes and carbon nanotube-based systems related to biomedical applications. Results: Adding to this, a short chronological description of these tiny yet powerful particles given, followed by a discussion regarding their types, properties, methods of synthesis, scale-up, purification techniques and characterization aspects of carbon nanotubes. Conclusion: In the later part, the functionalization of carbon nanotubes was reviewed in detail, which is important to make them biocompatible and stable in biological systems and render them a great property of loading various biomolecules diagnostic and therapeutic moieties. Lastly, an inclusive description of the potential biomedical applications has been given followed by insights into the future.

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