scholarly journals Modulation of Mesenchymal Stem Cells Mechanosensing at Fluid Interfaces by Tailored Self‐Assembled Protein Monolayers

Small ◽  
2019 ◽  
Vol 15 (5) ◽  
pp. 1804640 ◽  
Author(s):  
Xiaofang Jia ◽  
Kosuke Minami ◽  
Koichiro Uto ◽  
Alice Chinghsuan Chang ◽  
Jonathan P. Hill ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluid Interfaces ◽  
Protein Monolayers ◽  
Self Assembled

scholarly journals A microRNA delivery carrier for hepatic carcinoma therapy using layer-by-layer self-assembled mesenchymal stem cells

2020 ◽  
Vol 9 (9) ◽  
pp. 5380-5389
Author(s):  
Chunmin Li ◽  
Xin Li ◽  
Fengsheng Li ◽  
Dawei Wang ◽  
Jin Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Layer By Layer ◽  
Hepatic Carcinoma ◽  
Self Assembled ◽  
Microrna Delivery

scholarly journals Study on a 3D-Bioprinted Tissue Model of Self-Assembled Nanopeptide Hydrogels Combined With Adipose-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Guanzhou Zhou ◽  
Ailing Tian ◽  
Xin Yi ◽  
Lufeng Fan ◽  
Wenchong Shao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Primary Cultures ◽  
Mixed Solution ◽  
Control Groups ◽  
Alizarin Red ◽  
Tissue Model ◽  
Self Assembled ◽  
Experimental Group

Objective: This study aimed to observe the cell growth status and multidirectional differentiation ability in a 3D-bioprinted tissue model of self-assembled nanopeptides and human adipose-derived mesenchymal stem cells (Ad-MSCs).Methods: Primary Ad-MSCs were isolated, cultured, and identified by flow cytometry. Tissue models were printed via 3D bioprinting technology using a “biological ink” consisting of a mixed solution of self-assembled nanopeptides and Ad-MSCs. Ad-MSCs were induced into osteogenic, adipogenic, and endothelial differentiation and compared with the control groups by staining.Results: The nanopeptide fiber was 10–30 nm in diameter and 200–500 nm in length under the atomic-force microscope. It had the characteristics of nano-scale materials. Flow cytometry showed that the isolated and cultured cells were positive for CD29 (98.51%), CD90 (97.87%), and CD166 (98.32%) but did not express CD31 (1.58%), CD34 (2.42%), CD45 (2.95%), or human leukocyte antigen (HLA)-DR (0.53%), consistent with the immunophenotype of Ad-MSCs. Then, a tissue model was printed using the biological ink, followed by induction of differentiation of Ad-MSCs within the tissue model. Alizarin red S staining showed the formation of calcium nodules in the osteogenesis induction experimental group, and oil red O stained lipid droplets in Ad-MSCs in the adipogenesis induction experimental group, whereas the two control groups were not stained.Conclusion: Ad-MSCs from primary cultures have the characteristics of stem cells. Self-assembled nanopeptide hydrogel is a good tissue engineering material that can serve as an extracellular matrix. Ad-MSCs in the 3D-printed tissue model using a biological ink consisting of a mixed solution of self-assembled nanopeptides and Ad-MSCs grew well and still had strong differentiation ability.

scholarly journals Self‐Assembled Type I Collagen‐Apatite Fibers with Varying Mineralization Extent and Luminescent Terbium Promote Osteogenic Differentiation of Mesenchymal Stem Cells

2020 ◽  
pp. 2000319
Author(s):  
Ismael Romero‐Castillo ◽  
Elena López‐Ruiz ◽  
Jorge Fernando Fernández‐Sánchez ◽  
Juan Antonio Marchal ◽  
Jaime Gómez‐Morales
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Type I Collagen ◽  
Type I ◽  
Self Assembled

Self-Assembly of a Multi-Functional Hydrogel from a Branched Peptide Amphiphile and Its Effects on Bone Marrow Mesenchymal Stem Cells

2020 ◽  
Vol 10 (12) ◽  
pp. 1731-1737
Author(s):  
Li Yixiu ◽  
Yin Peiyi ◽  
Wu Kai ◽  
Wang Xiaomei ◽  
Song Yulin
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Solid Phase ◽  
Cell Phenotype ◽  
Neurofilament Protein ◽  
Acetoxymethyl Ester ◽  
Peptide Amphiphile ◽  
Marrow Mesenchymal Stem Cells ◽  
Self Assembled

In this work, a branched peptide amphiphile (B-PA) presenting RGD and IKVAV motifs was fabricated by solid-phase peptide synthesis and self-assembled into a nanofiber hydrogel in which rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded and cultured for seven days. Specifically, 1 wt% B-PA was self-assembled into a nanofiber hydrogel with the addition of culture medium and observed using transmission electron microscopy. The B-PA with a molecular weight of 2191.72 and a purity >95% self-assembled into nanofibers with diameters from 6 to 8 nm and lengths ranging from hundreds of nanometers to several micrometers. BMSCs were acquired from rabbits using differential adherence methods and identified by flow cytometry for cell phenotype. The cells were stained with calcein acetoxymethyl ester/propidium iodide to assess cell viability, CCK-8 to assess cell cytotoxicity and proliferation, and Hochest 33342 to assess cell adhesion. They were also immunofluorescently labeled with microtubule-associated protein-2 (MAP-2), neurofilament protein (NF), and glial fibrillary acidic protein (GFAP) to assess cell transdifferentiation. The B-PA hydrogel provided platform upon which the CD29+/44+ cells adhered and proliferated, and it induced the transdifferentiation of cells into neural cells expressing the markers MAP-2, NF, and GFAP. The hydrogel exhibited good cytocompatibility and multiple functions, and may therefore serve as a scaffold for neural tissue engineering.

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