scholarly journals Magnetic Nanoparticles: High-Throughput, Protein-Targeted Biomolecular Detection Using Frequency-Domain Faraday Rotation Spectroscopy (Small 12/2017)

Small ◽  
2017 ◽  
Vol 13 (12) ◽  
Author(s):  
Richard J. Murdock ◽  
Shawn A. Putnam ◽  
Soumen Das ◽  
Ankur Gupta ◽  
Elyse D. Z. Chase ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Frequency Domain ◽  
High Throughput ◽  
Faraday Rotation ◽  
Biomolecular Detection

scholarly journals High-Throughput, Protein-Targeted Biomolecular Detection Using Frequency-Domain Faraday Rotation Spectroscopy

Small ◽  
2017 ◽  
Vol 13 (12) ◽  
pp. 1602862 ◽  
Author(s):  
Richard J. Murdock ◽  
Shawn A. Putnam ◽  
Soumen Das ◽  
Ankur Gupta ◽  
Elyse D. Z. Chase ◽  
...  
Keyword(s):  
Frequency Domain ◽  
High Throughput ◽  
Faraday Rotation ◽  
Biomolecular Detection

Debye-Based Frequency-Domain Magnetization Model for Magnetic Nanoparticles in Magnetic Particle Spectroscopy

2015 ◽  
Vol 51 (2) ◽  
pp. 1-4 ◽  
Author(s):  
Thilo Wawrzik ◽  
Takashi Yoshida ◽  
Meinhard Schilling ◽  
Frank Ludwig
Keyword(s):  
Magnetic Nanoparticles ◽  
Frequency Domain ◽  
Magnetic Particle

Simultaneous detection of multiple viruses based on chemiluminescence and magnetic separation

2017 ◽  
Vol 5 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Zeeshan Ali ◽  
Jiuhai Wang ◽  
Yongjun Tang ◽  
Bin Liu ◽  
Nongyue He ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
High Throughput ◽  
Dna Hybridization ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Reliable Detection ◽  
Automated Platform ◽  
Modified Magnetic Nanoparticles ◽  
Surface Modified ◽  
Multiple Pathogens

In this report, a DNA hybridization based chemiluminescent detection method has been proposed for reliable detection of multiple pathogens. The use of surface modified magnetic nanoparticles can help to integrate this system into an automated platform for high throughput applications.

scholarly journals MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically-encoded hydrogel beads using a simple single-layer microfluidic device

2020 ◽  
Author(s):  
Yinnian Feng ◽  
Adam K. White ◽  
Jamin B. Hein ◽  
Eric A. Appel ◽  
Polly M. Fordyce
Keyword(s):  
Magnetic Nanoparticles ◽  
Functional Groups ◽  
High Throughput ◽  
Single Layer ◽  
Flow Focusing ◽  
Polymer Mixtures ◽  
Simple Method ◽  
Hydrogel Beads ◽  
Basic Biology ◽  
A Minor

AbstractWidespread adoption of bead-based multiplexed bioassays requires the ability to easily synthesize encoded microspheres and conjugate analytes of interest to their surface. Here, we present a simple method (MRBLEs 2.0) for efficient high-throughput generation of microspheres with ratiometric barcode lanthanide encoding (MRBLEs) bearing functional groups for downstream bioconjugation. Bead production in MRBLEs 2.0 relies on manual mixing of lanthanide/polymer mixtures (each of which comprises a unique spectral code) followed by droplet generation using single-layer, parallel flow-focusing devices and off-chip batch polymerization of droplets into beads. To streamline downstream analyte coupling, MRBLEs 2.0 crosslinks copolymers bearing functional groups on the bead surface simultaneously during bead generation. Using the MRBLEs 2.0 pipeline, we generate monodisperse MRBLEs containing 48 distinct well-resolved spectral codes in high-throughput (>150,000/min and can be boosted to 450,000/min). We further demonstrate the efficient conjugation of oligonucleotides and entire proteins to carboxyl MRBLEs and biotin to amino MRBLEs. Finally, we show that MRBLEs can also be magnetized via simultaneous incorporation of magnetic nanoparticles with only a minor decrease in the potential code space. We anticipate that MRBLEs 2.0 can be directly applied towards a wide variety of downstream assays from basic biology to diagnostics and other translational research.

Nanosized aptameric cavities imprinted on the surface of magnetic nanoparticles for high-throughput protein recognition

2018 ◽  
Vol 185 (4) ◽  
Author(s):  
Elham Shoghi ◽  
Seyede Zohreh Mirahmadi-Zare ◽  
Razieh Ghasemi ◽  
Matine Asghari ◽  
Mansour Poorebrahim ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
High Throughput ◽  
Protein Recognition

scholarly journals Frequency-domain birefringence measurement of biological binding to magnetic nanoparticles

2008 ◽  
Vol 320 (18) ◽  
pp. 2279-2283 ◽  
Author(s):  
Benjamin Y. Ku ◽  
Mei-Lin Chan ◽  
Zhiya Ma ◽  
David A. Horsley
Keyword(s):  
Magnetic Nanoparticles ◽  
Frequency Domain
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