Molecular Imprinting for Substrate Selectivity and Enhanced Activity of Enzyme Mimics

Small ◽  
2016 ◽  
Vol 13 (7) ◽  
pp. 1602730 ◽  
Author(s):  
Zijie Zhang ◽  
Biwu Liu ◽  
Juewen Liu
Keyword(s):  
Molecular Imprinting ◽  
Substrate Selectivity ◽  
Enzyme Mimics ◽  
Enhanced Activity

Molecularly imprinted peptide-based enzyme mimics with enhanced activity and specificity

Soft Matter ◽  
2020 ◽  
Vol 16 (30) ◽  
pp. 7033-7039 ◽  
Author(s):  
Jingyi Li ◽  
Mingjie Zhu ◽  
Mengfan Wang ◽  
Wei Qi ◽  
Rongxin Su ◽  
...  
Keyword(s):  
Molecular Imprinting ◽  
Enzyme Mimics ◽  
Molecularly Imprinted ◽  
Peptide Assembly ◽  
Enhanced Activity

Peroxidase (POD)-mimicking catalysts with enhanced activity and specificity were constructed based on the strategy of peptide assembly and molecular imprinting.

Catalytic gold–platinum alloy nanoparticles and a novel glucose oxidase mimic with enhanced activity and selectivity constructed by molecular imprinting

2019 ◽  
Vol 11 (36) ◽  
pp. 4586-4592 ◽  
Author(s):  
Fan Lin ◽  
Tian Yushen ◽  
Lou Doudou ◽  
Wu Haoan ◽  
Cui Yan ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Glucose Oxidase ◽  
Molecular Imprinting ◽  
Alloy Nanoparticles ◽  
Substrate Selectivity ◽  
Platinum Alloy ◽  
Enhanced Activity ◽  
Molecular Imprinting Technology ◽  
Gold Platinum

Based on the research of the oxidase-like activity of various Au-based nanostructures, a novel AuPtNPs based GOD mimic with substrate-selectivity and an enhanced catalytic activity was constructed by molecular imprinting technology.

Intracellular delivery of a molecularly imprinted peroxidase mimicking DNAzyme for selective oxidation

2018 ◽  
Vol 5 (4) ◽  
pp. 738-744 ◽  
Author(s):  
Zijie Zhang ◽  
Juewen Liu
Keyword(s):  
Selective Oxidation ◽  
Molecular Imprinting ◽  
Intracellular Delivery ◽  
Enzyme Mimics ◽  
Molecularly Imprinted ◽  
Selective Catalysis ◽  
In Cells

Molecular imprinting of enzyme mimics allows delivery and selective catalysis and protection of the enzyme in cells.

The Role of the Initiator System in the Synthesis of Acidic Multifunctional Nanoparticles Designed for Molecular Imprinting of Proteins

2020 ◽  
Vol 65 (1) ◽  
pp. 28-41
Author(s):  
Marwa Aly Ahmed ◽  
Júlia Erdőssy ◽  
Viola Horváth
Keyword(s):  
Acrylic Acid ◽  
Binding Affinity ◽  
Molecular Imprinting ◽  
Low Temperatures ◽  
Ammonium Persulfate ◽  
Sodium Bisulfite ◽  
Multifunctional Nanoparticles ◽  
High Affinity ◽  
Initiator System

Multifunctional nanoparticles have been shown earlier to bind certain proteins with high affinity and the binding affinity could be enhanced by molecular imprinting of the target protein. In this work different initiator systems were used and compared during the synthesis of poly (N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) nanoparticles with respect to their future applicability in molecular imprinting of lysozyme. The decomposition of ammonium persulfate initiator was initiated either thermally at 60 °C or by using redox activators, namely tetramethylethylenediamine or sodium bisulfite at low temperatures. Morphology differences in the resulting nanoparticles have been revealed using scanning electron microscopy and dynamic light scattering. During polymerization the conversion of each monomer was followed in time. Striking differences were demonstrated in the incorporation rate of acrylic acid between the tetramethylethylenediamine catalyzed initiation and the other systems. This led to a completely different nanoparticle microstructure the consequence of which was the distinctly lower lysozyme binding affinity. On the contrary, the use of sodium bisulfite activation resulted in similar nanoparticle structural homogeneity and protein binding affinity as the thermal initiation.

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