Monodisperse Emulsion Drop Microenvironments for Bacterial Biofilm Growth

Small ◽  
2015 ◽  
Vol 11 (32) ◽  
pp. 3954-3961 ◽  
Author(s):  
Connie B. Chang ◽  
James N. Wilking ◽  
Shin-Hyun Kim ◽  
Ho Cheung Shum ◽  
David A. Weitz
Keyword(s):  
Bacterial Biofilm ◽  
Biofilm Growth ◽  
Monodisperse Emulsion

Bifunctional Composites for Biofilms Modulation on Cervical Restorations

2021 ◽  
pp. 002203452110181
Author(s):  
A.A. Balhaddad ◽  
I.M. Garcia ◽  
L. Mokeem ◽  
M.S. Ibrahim ◽  
F.M. Collares ◽  
...  
Keyword(s):  
Free Energy ◽  
Surface Free Energy ◽  
Contact Angles ◽  
Bacterial Biofilm ◽  
Dental Composite ◽  
Log P ◽  
Rrna Gene ◽  
Cervical Lesions ◽  
Biofilm Growth ◽  
Biofilm Development

Cervical composites treating root carious and noncarious cervical lesions usually extend subgingivally. The subgingival margins of composites present poor plaque control, enhanced biofilm accumulation, and cause gingival irritation. A potential material to restore such lesions should combine agents that interfere with bacterial biofilm development and respond to acidic conditions. Here, we explore the use of new bioresponsive bifunctional dental composites against mature microcosm biofilms derived from subgingival plaque samples. The designed formulations contain 2 bioactive agents: dimethylaminohexadecyl methacrylate (DMAHDM) at 3 to 5 wt.% and 20 wt.% nanosized amorphous calcium phosphate (NACP) in a base resin. Composites with no DMAHDM and NACP were used as controls. The newly formulated 5% DMAHDM–20% NACP composite was analyzed by micro-Raman spectroscopy and transmission electron microscopy. The wettability and surface-free energy were also assessed. The inhibitory effect on the in vitro biofilm growth and the 16S rRNA gene sequencing of survival bacterial colonies derived from the composites were analyzed. Whole-biofilm metabolic activity, polysaccharide production, and live/dead images of the biofilm grown over the composites complement the microbiological assays. Overall, the designed formulations had higher contact angles with water and lower surface-free energy compared to the commercial control. The DMAHDM-NACP composites significantly inhibited the growth of total microorganisms, Porphyromonas gingivalis, Prevotella intermedia/nigrescens, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum by 3 to 5-log ( P < 0.001). For the colony isolates from control composites, the composition was typically dominated by the genera Veillonella, Fusobacterium, Streptococcus, Eikenella, and Leptotrichia, while Fusobacterium and Veillonella dominated the 5% DMAHDM–20% NACP composites. The DMAHDM-NACP composites contributed to over 80% of reduction in metabolic and polysaccharide activity. The suppression effect on plaque biofilms suggested that DMAHDM-NACP composites might be used as a bioactive material for cervical restorations. These results may propose an exciting path to prevent biofilm growth and improve dental composite restorations’ life span.

scholarly journals Oral streptococci growth on aging and non-aging esthetic restorations after radiotherapy

2010 ◽  
Vol 21 (4) ◽  
pp. 346-350 ◽  
Author(s):  
Adriana D. da Cruz ◽  
Karina Cogo ◽  
Cristiane de C. Bergamaschi ◽  
Frab N. Bóscolo ◽  
Francisco C. Groppo ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Biofilm ◽  
Oral Streptococci ◽  
Restorative Material ◽  
Biofilm Growth ◽  
Aged Group ◽  
Dental Resins ◽  
Colony Forming Units ◽  
Therapeutic Doses ◽  
Therapeutic Irradiation

The aim of this study was to examine Streptococcus mutans biofilm growth on both aged and non-aged restorative dental resins, which were submitted to therapeutic irradiation. Sixty-four disks of an esthetic restorative material (Filtek Supreme) were divided into 2 groups: aged group (AG) and a non-aged group (NAG). Each group was subdivided into 4 subgroups: non-irradiated and irradiated with 10Gy, 35Gy, and 70Gy. The biofilms were produced by Streptococcus mutans UA159 growing on both AG and NAG surfaces. The colony-forming units per mL (CFU/mL) were evaluated by the ANOVA and the Tukey LSD tests (α=0.05). AG presented smaller amounts of CFU/mL than the NAG before irradiation and after 10Gy of irradiation (p<0.05). AG irradiated with 35 and 70Gy showed increased amount of bacterial biofilm when compared to non-irradiated and 10Gy-irradiated disks (p<0.05). The exposure to ionizing radiation at therapeutic doses promoted changes in bacterial adherence of aged dental restorative material.

scholarly journals The development of biofilm architecture

2016 ◽  
Vol 472 (2188) ◽  
pp. 20150798 ◽  
Author(s):  
A. C. Fowler ◽  
T. M. Kyrke-Smith ◽  
H. F. Winstanley
Keyword(s):  
Bacterial Biofilm ◽  
Governing Equations ◽  
Biofilm Growth ◽  
One Dimensional ◽  
Direct Numerical Solution ◽  
The Common ◽  
Common Observation ◽  
The Stability ◽  
The One ◽  
A Free Boundary Problem

We extend the one-dimensional polymer solution theory of bacterial biofilm growth described by Winstanley et al . (2011 Proc. R. Soc. A 467 , 1449–1467 ( doi:10.1098/rspa.2010.0327 )) to deal with the problem of the growth of a patch of biofilm in more than one lateral dimension. The extension is non-trivial, as it requires consideration of the rheology of the polymer phase. We use a novel asymptotic technique to reduce the model to a free-boundary problem governed by the equations of Stokes flow with non-standard boundary conditions. We then consider the stability of laterally uniform biofilm growth, and show that the model predicts spatial instability; this is confirmed by a direct numerical solution of the governing equations. The instability results in cusp formation at the biofilm surface and provides an explanation for the common observation of patterned biofilm architectures.

scholarly journals Organo-Selenium-Containing Polyester Bandage Inhibits Bacterial Biofilm Growth on the Bandage and in the Wound

Biomedicines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 62
Author(s):  
Phat Tran ◽  
Tyler Enos ◽  
Keaton Luth ◽  
Abdul Hamood ◽  
Coby Ray ◽  
...  
Keyword(s):  
Wound Infection ◽  
Wound Dressing ◽  
Laser Scanning ◽  
Bacterial Biofilm ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Strains ◽  
Biofilm Growth ◽  
Biofilm Model

The dressing material of a wound plays a key role since bacteria can live in the bandage and keep re-infecting the wound, thus a bandage is needed that blocks biofilm in the bandage. Using an in vivo wound biofilm model, we examined the effectiveness of an organo-selenium (OS)-coated polyester dressing to inhibit the growth of bacteria in a wound. Staphylococcus aureus (as well as MRSA, Methicillin resistant Staph aureus), Stenotrophomonas maltophilia, Enterococcus faecalis, Staphylococcus epidermidis, and Pseudomonas aeruginosa were chosen for the wound infection study. All the bacteria were enumerated in the wound dressing and in the wound tissue under the dressing. Using colony-forming unit (CFU) assays, over 7 logs of inhibition (100%) was found for all the bacterial strains on the material of the OS-coated wound dressing and in the tissue under that dressing. Confocal laser scanning microscopy along with IVIS spectrum in vivo imaging confirmed the CFU results. Thus, the dressing acts as a reservoir for a biofilm, which causes wound infection. The same results were obtained after soaking the dressing in PBS at 37 °C for three months before use. These results suggest that an OS coating on polyester dressing is both effective and durable in blocking wound infection.

Microstructuring and Biofunctionalization of Alumina Surfaces to Enhance Abrasion Resistance and Suppress Bacterial Biofilm Growth

2008 ◽  
Author(s):  
Laura Treccani ◽  
Kurosch Rezwan
Keyword(s):  
Bacterial Adhesion ◽  
Bacterial Biofilm ◽  
Transport Systems ◽  
Biofilm Growth ◽  
Abrasive Particles ◽  
Microstructured Surfaces ◽  
Viable Bacteria ◽  
Feasible Alternative ◽  
Fundamental Research ◽  
Patterning Technique

The design and fabrication of alumina microstructured surfaces that simultaneously present high mechanical and chemical features and do not suffer biofouling are here reported. An aerosol based patterning technique was employed to fabricate alumina microstructures directly on alumina surfaces with the aim to enhance wear and chemical resistance. Microstructured alumina surfaces were subsequently biofunctionalised with antibacterial biomolecules to inhibit bacterial adhesion. Lysozyme, an antibacterial enzyme commonly found in body secretions, was used as antibacterial agent and directly deposited onto microstructured alumina surfaces. Lysozyme-biofunctionalised microstructured alumina surfaces were tested at flow condition using abrasive particles and viable bacteria. The very preliminary results showed that alumina microstructures presented high resistance against mechanical abrasion and that bacterial biofilm formation could be suppressed. In particular alumina microstructures protected lysozyme molecules from desorption and loss of enzymatic activity. Such biofunctionalised microstructures present a promising system for fundamental research in the field of biomolecule adsorption on surfaces and maybe a feasible alternative e. g. to protect surfaces of water transport systems where abrasive particles and microorganisms are present.

scholarly journals Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism

2017 ◽  
Author(s):  
Damien Keogh ◽  
Ling Ning Lam ◽  
Lucinda E. Doyle ◽  
Artur Matysik ◽  
Shruti Pavagadhi ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Enterococcus Faecalis ◽  
Bacterial Biofilm ◽  
Extracellular Electron Transfer ◽  
Bacterial Cells ◽  
Free Living ◽  
Biofilm Growth ◽  
Metabolic Versatility ◽  
Tract Infections ◽  
Biofilm Matrix

AbstractEnterococci are important human commensals and significant opportunistic pathogens associated with endocarditis, urinary tract infections, wound and surgical site infections, and medical device associated infections. These infections often become chronic upon the formation of biofilm. The biofilm matrix establishes properties that distinguish this state from free-living bacterial cells and increase tolerance to antimicrobial interventions. The metabolic versatility of the Enterococci is reflected in the diversity and complexity of environments and communities in which they thrive. Understanding metabolic factors governing colonization and persistence in different host niches can reveal factors influencing the transition from commensal to opportunistic pathogen. Here, we report a new form of iron-dependent metabolism for Enterococcus faecalis where, in the absence of heme, respiration components can be utilised for extracellular electron transfer (EET). Iron augments E. faecalis biofilm growth and generates alterations in biofilm matrix, cell spatial distribution, and biofilm matrix properties. We identify the genes involved in iron-augmented biofilm growth and show that it occurs by promoting EET to iron within biofilm.SignificanceBacterial metabolic versatility is often key in dictating the outcome of host-pathogen interactions, yet determinants of metabolic shifts are difficult to resolve. The bacterial biofilm matrix provides the structural and functional support that distinguishes this state from free-living bacterial cells. Here, we show that the biofilm matrix provides access to resources necessary for metabolism and growth which are otherwise inaccessible in the planktonic state. Our data shows that in the absence of heme, components of Enterococcus faecalis respiration (l-lactate dehydrogenase and acetaldehyde dehydrogenase) may function as initiators of EET through the cytoplasmic membrane quinone pool and utilize matrix-associated iron to carry out EET. The presence of iron resources within the biofilm matrix leads to enhanced biofilm growth.

scholarly journals Modeling of symbiotic bacterial biofilm growth with an example of the Streptococcus-Veillonella sp. system

2020 ◽  
Author(s):  
Dianlei Feng ◽  
Insa Neuweiler ◽  
Regina Nogueira ◽  
Udo Nackenhorst
Keyword(s):  
Numerical Solutions ◽  
Bacterial Biofilm ◽  
Thickness Direction ◽  
Biomass Distribution ◽  
Biofilm Growth ◽  
Numerical Examples ◽  
Biofilm Thickness ◽  
Coupled Partial Differential Equations ◽  
Numerical Investigations ◽  
Numerical Strategy

AbstractWe present a multi-dimensional continuum mathematical model for modeling the growth of a symbiotic biofilm system. We take a dual-species namely, the Streptococcus - Veillonella sp. biofilm system as an example for numerical investigations. The presented model describes both the cooperation and competition between these species of bacteria. The coupled partial differential equations are solved by using an integrative finite element numerical strategy. Numerical examples are carried out for studying the evolution and distribution of the bio-components. The results demonstrate that the presented model is capable of describing the symbiotic behavior of the biofilm system. However, homogenized numerical solutions are observed locally. To study the homogenization behavior of the model, numerical investigations regarding on how random initial biomass distribution influences the homogenization process are carried out. We found that a smaller correlation length of the initial biomass distribution leads to faster homogenization of the solution globally, however, shows more fluctuated biomass profiles along the biofilm thickness direction. More realistic scenarios with bacteria in patches are also investigated numerically in this study.

scholarly journals Bacterial Biofilm Growth on 3D-Printed Materials

2021 ◽  
Vol 12 ◽  
Author(s):  
Donald C. Hall ◽  
Phillip Palmer ◽  
Hai-Feng Ji ◽  
Garth D. Ehrlich ◽  
Jarosław E. Król
Keyword(s):  
Medical Devices ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Chronic Infections ◽  
Biofilm Growth ◽  
Wide Range ◽  
3D Printed ◽  
Printed Materials

Recent advances in 3D printing have led to a rise in the use of 3D printed materials in prosthetics and external medical devices. These devices, while inexpensive, have not been adequately studied for their ability to resist biofouling and biofilm buildup. Bacterial biofilms are a major cause of biofouling in the medical field and, therefore, hospital-acquired, and medical device infections. These surface-attached bacteria are highly recalcitrant to conventional antimicrobial agents and result in chronic infections. During the COVID-19 pandemic, the U.S. Food and Drug Administration and medical officials have considered 3D printed medical devices as alternatives to conventional devices, due to manufacturing shortages. This abundant use of 3D printed devices in the medical fields warrants studies to assess the ability of different microorganisms to attach and colonize to such surfaces. In this study, we describe methods to determine bacterial biofouling and biofilm formation on 3D printed materials. We explored the biofilm-forming ability of multiple opportunistic pathogens commonly found on the human body including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus to colonize eight commonly used polylactic acid (PLA) polymers. Biofilm quantification, surface topography, digital optical microscopy, and 3D projections were employed to better understand the bacterial attachment to 3D printed surfaces. We found that biofilm formation depends on surface structure, hydrophobicity, and that there was a wide range of antimicrobial properties among the tested polymers. We compared our tested materials with commercially available antimicrobial PLA polymers.

scholarly journals Essential Oils Biofilm Modulation Activity, Chemical and Machine Learning Analysis—Application on Staphylococcus aureus Isolates from Cystic Fibrosis Patients

2020 ◽  
Vol 21 (23) ◽  
pp. 9258
Author(s):  
Rosanna Papa ◽  
Stefania Garzoli ◽  
Gianluca Vrenna ◽  
Manuela Sabatino ◽  
Filippo Sapienza ◽  
...  
Keyword(s):  
Machine Learning ◽  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Machine Learning Algorithms ◽  
Bacterial Biofilm ◽  
Active Components ◽  
Biofilm Growth ◽  
Analysis Application ◽  
Multicomponent Nature

Bacterial biofilm plays a pivotal role in chronic Staphylococcus aureus (S. aureus) infection and its inhibition may represent an important strategy to develop novel therapeutic agents. The scientific community is continuously searching for natural and “green alternatives” to chemotherapeutic drugs, including essential oils (EOs), assuming the latter not able to select resistant strains, likely due to their multicomponent nature and, hence, multitarget action. Here it is reported the biofilm production modulation exerted by 61 EOs, also investigated for their antibacterial activity on S. aureus strains, including reference and cystic fibrosis patients’ isolated strains. The EOs biofilm modulation was assessed by Christensen method on five S. aureus strains. Chemical composition, investigated by GC/MS analysis, of the tested EOs allowed a correlation between biofilm modulation potency and putative active components by means of machine learning algorithms application. Some EOs inhibited biofilm growth at 1.00% concentration, although lower concentrations revealed different biological profile. Experimental data led to select antibiofilm EOs based on their ability to inhibit S. aureus biofilm growth, which were characterized for their ability to alter the biofilm organization by means of SEM studies.

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