Inorganic-Organic Hybrid Nanoprobe for NIR-Excited Imaging of Hydrogen Sulfide in Cell Cultures and Inflammation in a Mouse Model

Small ◽  
2014 ◽  
Vol 10 (23) ◽  
pp. 4874-4885 ◽  
Author(s):  
Yi Zhou ◽  
Wangqiao Chen ◽  
Jixin Zhu ◽  
Wenbo Pei ◽  
Chengyuan Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Cell Cultures ◽  
Organic Hybrid ◽  
Hybrid Nanoprobe

scholarly journals Nanoprobes: Inorganic-Organic Hybrid Nanoprobe for NIR-Excited Imaging of Hydrogen Sulfide in Cell Cultures and Inflammation in a Mouse Model (Small 23/2014)

Small ◽  
2014 ◽  
Vol 10 (23) ◽  
pp. 4802-4802 ◽  
Author(s):  
Yi Zhou ◽  
Wangqiao Chen ◽  
Jixin Zhu ◽  
Wenbo Pei ◽  
Chengyuan Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Cell Cultures ◽  
Organic Hybrid ◽  
Hybrid Nanoprobe

scholarly journals Risk Assessment for Use of a Porcine Circovirus-Contaminated Reagent in a Barrier Maintained Rodent Colony

2020 ◽  
Vol 59 (5) ◽  
pp. 575-579
Author(s):  
Norman C Peterson ◽  
Aaron A Berlin
Keyword(s):  
Risk Assessment ◽  
Mouse Model ◽  
Cell Cultures ◽  
Direct Contact ◽  
Model Development ◽  
Porcine Circovirus ◽  
Laboratory Mice ◽  
Wild Mice ◽  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase

A proposal for the use of porcine pancreatic elastase (PPE) to develop a mouse model of pulmonary emphysema raised concerns about introducing contaminating porcine viruses into our barrier facility. Porcine Circovirus (PCV) is a known contaminant of vaccines and cell cultures that have been exposed to porcine-derived reagents. Endemic infection of PCV3 in laboratory mice has been reported, and some evidence supports natural PCV infection in wild mice. PPE samples from 2 different vendors tested positive for DNA from both PCV2 and 3. To allow model development with these reagents to proceed, we developed a protocol that would meet scientific objectives, minimize exposure of mice, and provide information on the potential for the virus to spread. Five d after BALB/c mice received intralaryngeal administration of PPE, lungs were harvested and analyzed for evidence of disease. Tissues from other major organs were submitted to test for disseminated PCV2 and 3 DNA. Similarly, tissues (including lungs) from direct contact nude sentinel mice were analyzed for the presence of the virus. To evaluate the possibility of endemic PCV2/3 infection, we also surveyed non-porcine reagent exposed mice on other studies. PCV2 and 3 was not detected in any of the tissues submitted. Although this study provided no evidence of infection and transmission of PCV2/3 from the contaminated PPE sample over the 5 d study, further work is needed to understand the risks and impact of introducing PCV contaminated cells or reagents into barrier maintained rodent colonies.

scholarly journals Reductions in Hydrogen Sulfide Precede Onset of Mitochondrial Quality Control in the Corneal Kindled Mouse Model of Epilepsy

2021 ◽  
Author(s):  
Christi Cho ◽  
Maxwell Zeigler ◽  
Stephanie Mizuno ◽  
Richard S. Morrison ◽  
Rheem Totah ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Membrane Integrity ◽  
Brain Homogenate ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Mitochondrial Stress ◽  
Acute Seizures ◽  
Related Proteins

Epilepsy is a heterogenous neurological disorder characterized by recurrent unprovoked seizures, mitochondrial stress, and neurodegeneration. Hydrogen sulfide (H2S), a gasotransmitter, promotes mitochondrial function and biogenesis, elicits neuromodulation and neuroprotection, and may acutely suppress seizures. A major gap in knowledge remains in understanding the role of mitochondrial dysfunction and progressive changes in H2S levels following acute seizures and during epileptogenesis. We thus sought to quantify changes in H2S and its methylated metabolite (MeSH) via LC-MS/MS subsequent to acute maximal electroshock and 6 Hz 44 mA seizures in mice, as well as in the corneal kindled mouse model of chronic seizures. Plasma H2S was acutely reduced after a maximal electroshock seizure. H2S or MeSH levels in whole brain homogenate and expression of related genes in corneal kindled mice were not altered. However, plasma H2S and MeSH levels were significantly lower during kindling, but not after established kindling. Morever, we demonstrated a time-dependent increase in expression of mitochondrial membrane integrity-related proteins, Opa1, Mfn2, Drp1, and Mff during kindling, which did not correlate with gene expression. Taken together, short-term reductions in plasma H2S could be a novel biomarker for seizures. Future studies should further define the role of H2S and mitochondrial stress in epilepsy.

The Inhibitory Role of Hydrogen Sulfide in Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma

2013 ◽  
Vol 182 (4) ◽  
pp. 1188-1195 ◽  
Author(s):  
Gensheng Zhang ◽  
Peipei Wang ◽  
Guangdong Yang ◽  
Qiuhui Cao ◽  
Rui Wang
Keyword(s):  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Airway Hyperresponsiveness ◽  
Inhibitory Role

scholarly journals Hydrogen sulfide inhibits NLRP3 inflammasome activation and reduces cytokine production bothin vitroand in a mouse model of inflammation

2017 ◽  
Vol 293 (7) ◽  
pp. 2546-2557 ◽  
Author(s):  
Mariela Castelblanco ◽  
Jérôme Lugrin ◽  
Driss Ehirchiou ◽  
Sonia Nasi ◽  
Isao Ishii ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Nlrp3 Inflammasome ◽  
Cytokine Production ◽  
Inflammasome Activation ◽  
Nlrp3 Inflammasome Activation

scholarly journals Hydrogen sulfide enhances adult neurogenesis in a mouse model of Parkinson’s disease

2021 ◽  
Vol 16 (7) ◽  
pp. 1353
Author(s):  
Li Zhang ◽  
Chen Qiao ◽  
Min Wang ◽  
Juan-Juan Tang ◽  
Lin-Xiao Wang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Adult Neurogenesis

The Effects of Hydrogen Sulfide on Adipocyte Viability in Human Adipocyte and Adipocyte-Derived Mesenchymal Stem Cell Cultures Under Ischemic Conditions

2015 ◽  
Vol 75 (6) ◽  
pp. 657-665 ◽  
Author(s):  
Andac Aykan ◽  
Serdar Ozturk ◽  
Ismail Sahin ◽  
Ferit Avcu ◽  
Rahsan Ilikçi Sagkan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hydrogen Sulfide ◽  
Mesenchymal Stem Cell ◽  
Cell Cultures ◽  
Human Adipocyte

scholarly journals Profound Chemopreventative Effects of a Hydrogen Sulfide-Releasing NSAID in the APCMin/+ Mouse Model of Intestinal Tumorigenesis

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0147289 ◽  
Author(s):  
Mark Paul-Clark ◽  
Wagdi Elsheikh ◽  
Nicholas Kirkby ◽  
Melissa Chan ◽  
Pallavi Devchand ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Mouse Model ◽  
Intestinal Tumorigenesis
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