Direct Binding of a Redox Protein for Single-Molecule Electron Transfer Measurements

Small ◽  
2012 ◽  
Vol 8 (15) ◽  
pp. 2341-2344 ◽  
Author(s):  
Eduardo A. Della Pia ◽  
J. Emyr Macdonald ◽  
Martin Elliott ◽  
D. Dafydd Jones
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Redox Protein ◽  
Direct Binding

scholarly journals Fast electron transfer through a single molecule natively structured redox protein

Nanoscale ◽  
2012 ◽  
Vol 4 (22) ◽  
pp. 7106 ◽  
Author(s):  
Eduardo Antonio Della Pia ◽  
Qijin Chi ◽  
J. Emyr Macdonald ◽  
Jens Ulstrup ◽  
D. Dafydd Jones ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Fast Electron ◽  
Redox Protein

The Single-Molecule Conductance and Electrochemical Electron-Transfer Rate Are Related by a Power Law

ACS Nano ◽  
2013 ◽  
Vol 7 (6) ◽  
pp. 5391-5401 ◽  
Author(s):  
Emil Wierzbinski ◽  
Ravindra Venkatramani ◽  
Kathryn L. Davis ◽  
Silvia Bezer ◽  
Jing Kong ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Power Law ◽  
Transfer Rate ◽  
Electron Transfer Rate

Redox protein electron-transfer mechanisms: electrostatic interactions as a determinant of reaction site in c-type cytochromes

Biochemistry ◽  
1989 ◽  
Vol 28 (15) ◽  
pp. 6318-6322 ◽  
Author(s):  
G. Cheddar ◽  
T. E. Meyer ◽  
M. A. Cusanovich ◽  
C. D. Stout ◽  
Gordon Tollin
Keyword(s):  
Electron Transfer ◽  
Electrostatic Interactions ◽  
Reaction Site ◽  
Redox Protein ◽  
Protein Electron Transfer ◽  
Transfer Mechanisms

scholarly journals Single-molecule spectroelectrochemical cross-correlation during redox cycling in recessed dual ring electrode zero-mode waveguides

2017 ◽  
Vol 8 (8) ◽  
pp. 5345-5355 ◽  
Author(s):  
Donghoon Han ◽  
Garrison M. Crouch ◽  
Kaiyu Fu ◽  
Lawrence P. Zaino III ◽  
Paul W Bohn
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Cross Correlation ◽  
Zero Mode ◽  
Redox Cycling ◽  
Ring Electrode ◽  
Dual Ring

The ability of zero-mode waveguides (ZMW) to guide light into subwavelength-diameter nanoapertures has been exploited for studying electron transfer dynamics in zeptoliter-volume nanopores under single-molecule occupancy conditions.

scholarly journals Single occupancy spectroelectrochemistry of freely diffusing flavin mononucleotide in zero-dimensional nanophotonic structures

2015 ◽  
Vol 184 ◽  
pp. 101-115 ◽  
Author(s):  
Lawrence P. Zaino ◽  
Dane A. Grismer ◽  
Donghoon Han ◽  
Garrison M. Crouch ◽  
Paul W. Bohn
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Light Emission ◽  
Fluorescence Emission ◽  
Flavin Mononucleotide ◽  
Wide Field ◽  
Potential Sweep ◽  
Potential Control ◽  
Fluorescence Measurements ◽  
Electron Transfer Properties

Zero-mode waveguides (ZMW) have the potential to be powerful confinement tools for studying electron transfer dynamics at single molecule occupancy conditions. Flavin mononucleotide contains an isoalloxazine chromophore, which is fluorescent in the oxidized state (FMN) while the reduced state (FMNH2) exhibits dramatically lower light emission, i.e. a dark-state. This allows fluorescence emission to report the redox state of single FMN molecules, an observation that has been used previously to study single electron transfer events in surface-immobilized flavins and flavoenzymes, e.g. sarcosine oxidase, by direct wide-field imaging of ZMW arrays. Single molecule electron transfer dynamics have now been extended to the study of freely diffusing molecules using fluorescence measurements of Au ZMWs under single occupancy conditions. The Au in the ZMW serves both as an optical cladding layer and as the working electrode for potential control, thereby accessing single molecule electron transfer dynamics at μM concentrations. Consistent with expectations, the probability of observing single reduced molecules increases as the potential is scanned negative, Eappl < Eeq, and the probability of observing emitting oxidized molecules increases at Eappl > Eeq. Different single molecules exhibit different electron transfer properties as reflected in the position of Eeq and the distribution of Eeq among a population of FMN molecules. Two types of actively-controlled electroluminescence experiments were used: chronofluorometry experiments, in which the potential is alternately stepped between oxidizing and reducing potentials, and cyclic potential sweep fluorescence experiments, analogous to cyclic voltammetry, these latter experiments exhibiting a dramatic scan rate dependence with the slowest scan rates showing distinct intermediate states that are stable over a range of potentials. These states are assigned to flavosemiquinone species that are stabilized in the special environment of the ZMW nanopore.

Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

2013 ◽  
Vol 117 (42) ◽  
pp. 13015-13028 ◽  
Author(s):  
Dominik Haenni ◽  
Franziska Zosel ◽  
Luc Reymond ◽  
Daniel Nettels ◽  
Benjamin Schuler
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Photon Statistics ◽  
Single Molecule Fret

scholarly journals Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA

Microbiology ◽  
2005 ◽  
Vol 151 (1) ◽  
pp. 259-268 ◽  
Author(s):  
Birgit Baumgarth ◽  
Frank Wilco Bartels ◽  
Dario Anselmetti ◽  
Anke Becker ◽  
Robert Ros
Keyword(s):  
Binding Site ◽  
Single Molecule ◽  
Sinorhizobium Meliloti ◽  
Specific Binding ◽  
Dna Interaction ◽  
Dynamic Force ◽  
Sequence Motifs ◽  
Promoter Regions ◽  
Dissociation Kinetics ◽  
Direct Binding

The exopolysaccharide galactoglucan promotes the establishment of symbiosis between the nitrogen-fixing Gram-negative soil bacterium Sinorhizobium meliloti 2011 and its host plant alfalfa. The transcriptional regulator ExpG activates expression of galactoglucan biosynthesis genes by direct binding to the expA1, expG/expD1 and expE1 promoter regions. ExpG is a member of the MarR family of regulatory proteins. Analysis of target sequences of an ExpG(His)6 fusion protein in the exp promoter regions resulted in the identification of a binding site composed of a conserved palindromic region and two associated sequence motifs. Association and dissociation kinetics of the specific binding of ExpG(His)6 to this binding site were characterized by standard biochemical methods and by single-molecule spectroscopy based on the atomic force microscope (AFM). Dynamic force spectroscopy indicated a distinct difference in the kinetics between the wild-type binding sequence and two mutated binding sites, leading to a closer understanding of the ExpG–DNA interaction.

Efficiency of Interfacial Electron Transfer from Zn-Porphyrin Dyes into TiO2 Correlated to the Linker Single Molecule Conductance

2013 ◽  
Vol 117 (46) ◽  
pp. 24462-24470 ◽  
Author(s):  
Christian F. A. Negre ◽  
Rebecca L. Milot ◽  
Lauren A. Martini ◽  
Wendu Ding ◽  
Robert H. Crabtree ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Interfacial Electron Transfer
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