Label-Free Colorimetric Detection of Nucleic Acids Based on Target-Induced Shielding Against the Peroxidase-Mimicking Activity of Magnetic Nanoparticles

Small ◽  
2011 ◽  
Vol 7 (11) ◽  
pp. 1521-1525 ◽  
Author(s):  
Ki Soo Park ◽  
Moon Il Kim ◽  
Dae-Yeon Cho ◽  
Hyun Gyu Park
Keyword(s):  
Magnetic Nanoparticles ◽  
Nucleic Acids ◽  
Colorimetric Detection ◽  
Label Free

Yolk–shell nanostructured Fe3O4@C magnetic nanoparticles with enhanced peroxidase-like activity for label-free colorimetric detection of H2O2and glucose

Nanoscale ◽  
2017 ◽  
Vol 9 (13) ◽  
pp. 4508-4515 ◽  
Author(s):  
Na Lu ◽  
Min Zhang ◽  
Lei Ding ◽  
Jing Zheng ◽  
Caixia Zeng ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Colorimetric Detection ◽  
Label Free

scholarly journals Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ehsan Shokri ◽  
Morteza Hosseini ◽  
Mehdi D. Davari ◽  
Mohammad R. Ganjali ◽  
Maikel P. Peppelenbosch ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Label Free ◽  
Self Assembled ◽  
Novel Strategy

Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review

2004 ◽  
Vol 69 (4) ◽  
pp. 715-747 ◽  
Author(s):  
Miroslav Fojta
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Double Helix ◽  
Dna Structure ◽  
Electrochemical Analysis ◽  
Label Free ◽  
Helix Formation ◽  
Mercury Electrodes ◽  
Dna Strand ◽  
Dna Double Helix

This review is devoted to applications of mercury electrodes in the electrochemical analysis of nucleic acids and in studies of DNA structure and interactions. At the mercury electrodes, nucleic acids yield faradaic signals due to redox processes involving adenine, cytosine and guanine residues, and tensammetric signals due to adsorption/desorption of polynucleotide chains at the electrode surface. Some of these signals are highly sensitive to DNA structure, providing information about conformation changes of the DNA double helix, formation of DNA strand breaks as well as covalent or non-covalent DNA interactions with small molecules (including genotoxic agents, drugs, etc.). Measurements at mercury electrodes allow for determination of small quantities of unmodified or electrochemically labeled nucleic acids. DNA-modified mercury electrodes have been used as biodetectors for DNA damaging agents or as detection electrodes in DNA hybridization assays. Mercury film and solid amalgam electrodes possess similar features in the nucleic acid analysis to mercury drop electrodes. On the contrary, intrinsic (label-free) DNA electrochemical responses at other (non-mercury) solid electrodes cannot provide information about small changes of the DNA structure. A review with 188 references.

Colorimetric Detection of MPT64 Antibody Based on an Aptamer Adsorbed Magnetic Nanoparticles for Diagnosis of Tuberculosis

2019 ◽  
Vol 19 (2) ◽  
pp. 622-626 ◽  
Author(s):  
Hong Jae Cheon ◽  
Sang Min Lee ◽  
Seong-Rok Kim ◽  
Ho Yun Shin ◽  
Yiel Hea Seo ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Colorimetric Detection

scholarly journals Volumetric registration of magnetic nanoparticles for optimization of quantitative immunochromatographic assays for detection of small molecules

2018 ◽  
Vol 185 ◽  
pp. 10006 ◽  
Author(s):  
Natalia V. Guteneva ◽  
Sergey L. Znoyko ◽  
Alexey V. Orlov ◽  
Maxim P. Nikitin ◽  
Petr I. Nikitin
Keyword(s):  
Magnetic Nanoparticles ◽  
Small Molecules ◽  
Optical Methods ◽  
Magnetic Method ◽  
Label Free ◽  
Spectral Correlation ◽  
Entire Volume ◽  
Specificity And Sensitivity ◽  
Particle Quantification

Precise quantitative and highly sensitive detection of small molecules (haptens) is highly demanded in medicine, food quality control, in vitro diagnostics, criminalistics, environmental monitoring, etc. In the present work, the magnetic method of particle quantification and the optical methods of spectral correlation and spectral phase interferometry complement each other for optimization of a quantitative assay for measuring concentrations of small molecules. The assay employs magnetic nanoparticles as labels in rapid immunochromatographic format. The approach was demonstrated with fluorescein as a model molecule. The interferometric label-free biosensors were employed for selection of optimal reagents that produced high specificity and sensitivity. The method of magnetic particle quantification counted the magnetic labels over the entire volume of the immunochromatographic membrane to provide their distribution along the test strip. Such distribution was used for optimization of such parameters as concentrations of the used reagents and of antibody immobilized on the labels, amount of the labels and conjugates of haptens with protein carriers to realize the advanced quantitative immunochromatographic assay.

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