Preparation and In Vitro Biological Evaluation of Lawsone Loaded O‐Carboxymethyl Chitosan/Zinc Oxide Nanocomposite for Wound‐Healing Application

2020 ◽  
Vol 5 (9) ◽  
pp. 2710-2718
Author(s):  
Nagarajan Sakthiguru ◽  
Mohamed Aboobucker Sithique
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Biological Evaluation ◽  
Carboxymethyl Chitosan

Multifunctional Zinc Oxide/Silver Bimetallic Nanomaterial-Loaded Nanofibers for Enhanced Tissue Regeneration and Wound Healing

2021 ◽  
Vol 17 (9) ◽  
pp. 1840-1849
Author(s):  
Mao Li ◽  
Min Hu ◽  
Honglian Zeng ◽  
Bo Yang ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Tissue Regeneration ◽  
Ag Nanoparticles ◽  
Wound Care ◽  
Fluid Handling ◽  
Skin Repair ◽  
Wound Healing Effect

Native skin repair requires wound care products that not only protect the wound from bacterial infection, but also accelerate wound closure and minimize scarring. Nanomaterials have been widely applied for wound healing due to their multifunctional properties. In a previous study, we prepared and characterized electrospinning zinc oxide/silver/polyvinylpyrrolidone/polycaprolactone (ZnO/Ag/PVP/PCL) nanofibers using ZnO and Ag nanoparticles, and evaluated their antibacterial effect in vitro. In this work, further characterization studies were performed, which confirmed that the ZnO/Ag nanoparticles were physically embedded and evenly distributed in the ZnO/Ag/PVP/PCL nanofibers, enabling the sustained release of Ag and Zn. In addition, the bimetallic nanofibers showed satisfactory fluid handling and flexibility. In vivo wound healing and histology studies showed that the ZnO/Ag/PVP/PCL nanofibers had a better anti-inflammatory, skin tissue regeneration, and wound healing effect than monometallic nanofibers or a commercially available wound plaster (Yunnan Baiyao). Therefore, ZnO/Ag/PVP/PCL bimetallic nanofibers may be a safe, efficient biomedical dressing for wound healing.

Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro

2011 ◽  
Vol 10 (4) ◽  
pp. 369-378 ◽  
Author(s):  
Sikai Peng ◽  
Wanshun Liu ◽  
Baoqin Han ◽  
Jing Chang ◽  
Minyu Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Carboxymethyl Chitosan

scholarly journals Sponges of Carboxymethyl Chitosan Grafted with Collagen Peptides for Wound Healing

2019 ◽  
Vol 20 (16) ◽  
pp. 3890 ◽  
Author(s):  
Yu Cheng ◽  
Zhang Hu ◽  
Yuntao Zhao ◽  
Zuhao Zou ◽  
Sitong Lu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ftir Spectroscopy ◽  
Complete Healing ◽  
Carboxymethyl Chitosan ◽  
Pathological Examination ◽  
In Vitro Tests ◽  
Burn Care ◽  
Collagen Peptides ◽  
Fiber Deposition

Burns are physically debilitating and potentially fatal injuries. Two marine biomaterials, carboxymethyl chitosan (CMC) and collagen peptides (COP), have emerged as promising burn dressings. In this paper, sponges of carboxymethyl chitosan grafted with collagen peptide (CMC–COP) were prepared by covalent coupling and freeze drying. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were then used to characterize the prepared sponges. To evaluate the wound healing activity of the CMC–COP sponges, in vitro tests including cell viability scratch wound healing and scald wound healing experiments were performed in rabbits. Appearance studies revealed the porous nature of sponges and FTIR spectroscopy demonstrated the successful incorporation of COP into CMC. The in vitro scratch assay showed that treatment with CMC–COP sponges (at 100 μg/mL) had significant effects on scratch closure. For burn wounds treated with CMC–COP, regeneration of the epidermis and collagen fiber deposition was observed on day 7, with complete healing of the epidermis and wound on days 14 and 21, respectively. Based on the pathological examination by hematoxylin and eosinstaining, the CMC–COP group demonstrated pronounced wound healing efficiencies. These results confirmed that the CMC–COP treatment enhanced cell migration and promoted skin regeneration, thereby highlighting the potential application of these sponges in burn care.

Characterization and evaluation of a novel O-carboxymethyl chitosan films with Mimosa tenuiflora extract for skin regeneration and wound healing

2019 ◽  
Vol 35 (1) ◽  
pp. 39-56 ◽  
Author(s):  
Laura-Elizabeth Valencia-Gómez ◽  
Santos-Adriana Martel-Estrada ◽  
Claudia-Lucia Vargas-Requena ◽  
Juan-José Acevedo-Fernández ◽  
Claudia-Alejandra Rodríguez-González ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antimicrobial Properties ◽  
Contact Angle Measurement ◽  
Mechanical Tests ◽  
Angle Measurement ◽  
Carboxymethyl Chitosan ◽  
Skin Regeneration ◽  
Chitosan Films ◽  
Mimosa Tenuiflora

In this study, films of O-carboxymethyl chitosan with Mimosa tenuiflora extract were manufactured, characterized, and evaluated. In this work, both the synthesis of O-carboxymethyl chitosan and the extraction of the active ingredient of Mimosa tenuiflora extract from the cortex are described. First, the extract of Mimosa tenuiflora in water was obtained by precipitation with ethanol, filtering, and concentrating. Subsequently, a study was conducted of scratch wound healing to determine the optimal concentration of extract to be used in the manufacture of films. The produced O-carboxymethyl chitosan films and the Mimosa tenuiflora extract were mixed, and their chemical composition, tensile properties, and wettability were characterized by Fourier-transform infrared spectroscopy, mechanical tests, and contact angle measurement. The antimicrobial properties of the films were tested by turbidimetry using two types of bacteria. In addition, a study of the enzymatic degradation of the films with the enzyme lysozyme was performed. Finally, in vitro studies to assess the biocompatibility and cytotoxicity of films with fibroblastic cells were carried out as well as the kinetic analysis of healing in mice. It was found that the addition of Mimosa tenuiflora extract in the polymer matrix of the films made with O-carboxymethyl chitosan improves the proliferation of fibroblast and accelerates wound healing, thus providing a novel biomaterial for skin regeneration.

scholarly journals In vitro and in vivo biocompatibility and inflammation response of methacrylated and maleated hyaluronic acid for wound healing

RSC Advances ◽  
2020 ◽  
Vol 10 (53) ◽  
pp. 32183-32192
Author(s):  
Lijun Zhang ◽  
Ugo D'Amora ◽  
Alfredo Ronca ◽  
Yuanyuan Li ◽  
Xiaoying Mo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Biological Evaluation ◽  
Inflammation Response

From synthesis to the in vitro and in vivo biological evaluation of two types of hyaluronan derivatives.

scholarly journals Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing

2021 ◽  
Vol 8 (5) ◽  
Author(s):  
Alessia Ajovalasit ◽  
Carlos Redondo-Gómez ◽  
Maria Antonietta Sabatino ◽  
Babatunde O Okesola ◽  
Kristin Braun ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Electrostatic Interactions ◽  
Wound Repair ◽  
Biological Evaluation ◽  
Wound Dressings ◽  
Compression Tests ◽  
Peptide Amphiphile ◽  
Small Amplitude Oscillatory Shear

Abstract Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low concentration shows that CXG and PA-H3 co-assemble into nanofibres by hydrophobic and electrostatic interactions and further aggregate into nanofibre bundles and networks. At a higher concentration, CXG and PA-H3 yield hydrogels that have been characterized for their morphology by scanning electron microscopy and for the mechanical properties by small-amplitude oscillatory shear rheological measurements and compression tests at different CXG/PA-H3 ratios. A preliminary biological evaluation has been carried out both in vitro with HaCat cells and in vivo in a mouse model.

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