A Highly Sensitive and Stable SERS Sensor for Malachite Green Detection Based on Ag Nanoparticles In Situ Generated on 3D MoS 2 Nanoflowers

2020 ◽  
Vol 5 (1) ◽  
pp. 354-359
Author(s):  
Jiayu Jiang ◽  
Qihui Shen ◽  
Peng Xue ◽  
Hui Qi ◽  
Yunpeng Wu ◽  
...  
Keyword(s):  
Malachite Green ◽  
Ag Nanoparticles ◽  
Highly Sensitive

Polymethacrylic acid–facilitated nanofiber matrix loading Ag nanoparticles for SERS measurements

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38783-38790 ◽  
Author(s):  
Hui Yang ◽  
Cheng Zhi Huang
Keyword(s):  
Silver Nanoparticles ◽  
Malachite Green ◽  
Ag Nanoparticles ◽  
Organic Pollutant ◽  
Silver Ions ◽  
Polymethacrylic Acid ◽  
Ultrafine Fibers ◽  
Sers Substrates

By introducing polymethacrylic acid (PMAA), fairly uniform PMAA/poly(N-vinylpyrrolidone) (PVP) ultrafine fibers containing silver nanoparticles (AgNPs) for SERS substrates were successfully prepared via electrospinning by means of in situ photoreduction of silver ions. This makes the detection results reproducible with RSD values below 0.2% through the SERS signals to detect malachite green (MG), a significant environmental organic pollutant, for its genotoxicity.

Highly sensitive and colorimetric detection of hydrogen sulphide by in situ formation of Ag2S@Ag nanoparticles in polyelectrolyte multilayer film

RSC Advances ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 3508-3511 ◽  
Author(s):  
Hongxia Fu ◽  
Xinrui Duan
Keyword(s):  
Hydrogen Sulphide ◽  
Ag Nanoparticles ◽  
Multilayer Film ◽  
Colorimetric Detection ◽  
Ag Nps ◽  
Polyelectrolyte Multilayer ◽  
Highly Sensitive ◽  
In Situ Formation ◽  
Polyelectrolyte Multilayer Film

Ag ion reacted with H2S gas in polyelectrolyte multilayer film to form Ag2S nanoparticles that catalyze the formation of Ag NPs.

scholarly journals In situ preparation of Ag nanoparticles on silicon wafer as highly sensitive SERS substrate

RSC Advances ◽  
2018 ◽  
Vol 8 (6) ◽  
pp. 2887-2891 ◽  
Author(s):  
Xinglong Tu ◽  
Zheng Li ◽  
Jing Lu ◽  
Yanpeng Zhang ◽  
Guilin Yin ◽  
...  
Keyword(s):  
Silicon Wafer ◽  
Ag Nanoparticles ◽  
Sers Substrate ◽  
Surface Modifier ◽  
In Situ Preparation ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

An intensive surface enhanced Raman scattering (SERS) effect is realized by ordered Ag nanoparticles (NPs) in situ grown on silicon wafer directly using (3-aminopropyl) trimethoxysilane (APS) as both the surface modifier and reducing agent.

In Situ Formation of Ag Nanoparticles for Fiber Strain Sensors: Toward Textile-Based Wearable Applications

2021 ◽  
Author(s):  
Hwajoong Kim ◽  
Ammar Shaqeel ◽  
Solbi Han ◽  
Junseo Kang ◽  
Jieun Yun ◽  
...  
Keyword(s):  
Ag Nanoparticles ◽  
Strain Sensors ◽  
In Situ Formation

scholarly journals Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lenka Ulrychová ◽  
Pavel Ostašov ◽  
Marta Chanová ◽  
Michael Mareš ◽  
Martin Horn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Schistosoma Mansoni ◽  
Rna Sequencing ◽  
Serine Proteases ◽  
Expression Patterns ◽  
High Sensitivity ◽  
Host Parasite Interactions ◽  
Highly Sensitive ◽  
Host Parasite

Abstract Background The blood flukes of genus Schistosoma are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host–parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family Schistosoma mansoni serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in S. mansoni are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult S. mansoni by the high-sensitivity localization assay. Methodology Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques. Results FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of S. mansoni adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface. Conclusions The FISH-based assay provided spatial information about the expression of five SmSPs in adult S. mansoni females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host–parasite interactions. Graphic abstract

Sign in / Sign up

Export Citation Format

Share Document