A Turn‐On Fluorescence Probe for Rapid Detection of Hypochlorite in Living Cells and in Vitro

2019 ◽  
Vol 4 (27) ◽  
pp. 8021-8025 ◽  
Author(s):  
Ya Xie ◽  
Lijia Haung ◽  
Liqiang Yan ◽  
Jianping Li
Keyword(s):  
Rapid Detection ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Turn On

A naked-eye visible and turn-on fluorescence probe for Fe3+ and its bioimaging application in living cells

2016 ◽  
Vol 237 ◽  
pp. 501-508 ◽  
Author(s):  
Yaming Liu ◽  
Jinyu Zhang ◽  
Jiaxi Ru ◽  
Xiang Yao ◽  
Yang Yang ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Naked Eye ◽  
Turn On

scholarly journals A New Fluorescence Probe for Rapid Detection of Amyloid

2018 ◽  
Author(s):  
Nidhi Gour ◽  
Vivek Shinh Kshtriya ◽  
Bharti Koshti ◽  
Shradhey Gupta ◽  
khashti Ballabh Joshi ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Control Experiment ◽  
Fluorescence Probe ◽  
Photophysical Properties ◽  
Cost Effective ◽  
The Novel ◽  
High Quantum Yield ◽  
Turn On ◽  
Heat Treated ◽  
Amyloidogenic Peptides

<p>We report efficient synthesis and photophysical properties of a novel fluorescent pyridothiazole based construct by a facile methodology and its application as a sensor for rapid, selective as well as sensitive detection of amyloid fibres. The novel compound has exceptionally high quantum yield and sensitivity. The fluorescence of pyridothiazole construct is completely quenched (Turn-OFF) upon addition of cupric ions and the fluorescence is recovered on addition of amyloidogenic peptides like A<i>β</i><sub>42 </sub>(Turn-ON). The control experiment with native and heat treated BSA suggest that the new probe binds to beeta/<i>β</i> sheets present in amyloid. The new probe offers direct visualization of fluorescence ‘Turn-OFF’ ‘Turn-ON’ under long UV without the use of sophisticated instrumentation. Hence, a very simple, facile and cost-effective methodology for the rapid detection of amyloid is presented and it may in all possibility pave the way for the design of a better probe for sensing amyloids than conventional ThT.</p>

Fluorescence turn-on for the highly selective detection of nitric oxide in vitro and in living cells

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2600-2605 ◽  
Author(s):  
Xiaomei Liu ◽  
Shuang Liu ◽  
Gaolin Liang
Keyword(s):  
Nitric Oxide ◽  
Living Cells ◽  
Water Soluble ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A water-soluble, biocompatible, small molecular fluorescent turn-on probe was developed for the highly selective and sensitive detection of NO in vitro and in living cells.

A colorimetric and near-infrared fluorescent turn-on probe for in vitro and in vivo detection of thiophenols

2015 ◽  
Vol 7 (18) ◽  
pp. 7534-7539 ◽  
Author(s):  
Dehuan Yu ◽  
Qisong Zhai ◽  
Shengjun Yang ◽  
Guoqiang Feng
Keyword(s):  
Rapid Detection ◽  
Near Infrared ◽  
Turn On ◽  
In Vivo Detection

A colorimetric and NIR fluorescent turn-on probe was reported for rapid detection of thiophenols.

scholarly journals Selective cadmium fluorescence probe based on bis-heterocyclic molecule and its imaging in cells

2021 ◽  
Author(s):  
Masayori Hagimori ◽  
Yasushi Karimine ◽  
Naoko Mizuyama ◽  
Fumiko Hara ◽  
Takeshi Fujino ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Cell Membrane Permeability ◽  
Fluorescence Probes ◽  
Low Molecular Weight ◽  
Biological Applications ◽  
Turn On ◽  
Heterocyclic Molecule ◽  
Heterocyclic Moiety ◽  
In Cells

Abstract Fluorescence probes that selectively image cadmium are useful for detecting and tracking the amount of Cd2+ in cells and tissues. In this study, we designed and synthesized a novel Cd2+ fluorescence probe based on the pyridine-pyrimidine structure, 4-(methylsulfanyl)-6-(pyridin-2-yl)pyrimidin-2-amine (3), as a low-molecular-weight fluorescence probe for Cd2+. Compound 3 could successfully discriminate between Cd2+ and Zn2+ and exhibited a highly selective turn-on response toward Cd2+ over biologically related metal ions. The dissociation constant and detection limit of 5.4 x 10− 6 mol L− 1 and 4.4 × 10− 7 mol L− 1, respectively, were calculated using fluorescence titration experiments. Studies with closely related analogs showed that the bis-heterocyclic moiety of 3 acted as both a coordination site for Cd2+ and a fluorophore. Further, the methylsulfanyl group of compound 3 is essential for achieving selective and sensitive Cd2+ detection. Fluorescence microscopy studies using living cells revealed that the cell membrane permeability of compound 3 is sufficient to detect intracellular Cd2+. These results indicate that novel bis-heterocyclic molecule 3 has considerable potential as a fluorescence probe for Cd2+ in biological applications.

Development of a mitochondrial-targeted two-photon fluorescence turn-on probe for formaldehyde and its bio-imaging applications in living cells and tissues

2018 ◽  
Vol 42 (11) ◽  
pp. 8325-8329 ◽  
Author(s):  
An Xu ◽  
Yonghe Tang ◽  
Weiying Lin
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Two Photon ◽  
Turn On ◽  
Bio Imaging ◽  
Fluorescence Turn On ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Liver Tissues

The first mitochondrial-targeted two-photon fluorescence probe (MT-FA) for formaldehyde (FA) was engineered for monitoring FA in the mitochondria of living cells and liver tissues in one- and two-photon modes.

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