A New Approach of Controlled Single Step In Situ Fabrication of Graphene Composite Sensor for Simultaneous Sensing of Small Biomolecules in Human Urine

2019 ◽  
Vol 4 (5) ◽  
pp. 1640-1649 ◽  
Author(s):  
Nadeem Baig ◽  
Abdel‐Nasser Kawde ◽  
Mohamed Ibrahim
Keyword(s):  
Human Urine ◽  
Single Step ◽  
New Approach ◽  
Graphene Composite ◽  
In Situ Fabrication ◽  
Small Biomolecules ◽  
Simultaneous Sensing ◽  
Composite Sensor

Facile Single-step Technique for Simultaneous In Situ Fabrication of Ag/Ag2S–Polymer Nanocomposites via PPS Cyclization Route

2008 ◽  
Vol 37 (2) ◽  
pp. 194-195 ◽  
Author(s):  
Sujata Waghmare ◽  
Manish Shinde ◽  
Ramkrishna Gholap ◽  
N. Koteswara Rao ◽  
Tanay Seth ◽  
...  
Keyword(s):  
Polymer Nanocomposites ◽  
Single Step ◽  
In Situ Fabrication

In situ fabrication of platinum/graphene composite shell on polymer microspheres through reactive self-assembly and in situ reduction

2012 ◽  
Vol 48 (3) ◽  
pp. 1127-1133 ◽  
Author(s):  
Joon-Suk Oh ◽  
Nguyen Dang Luong ◽  
Tae-Seon Hwang ◽  
Jung-Pyo Hong ◽  
Young Kwan Lee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Composite Shell ◽  
Polymer Microspheres ◽  
In Situ Reduction ◽  
Graphene Composite ◽  
In Situ Fabrication

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

Sign in / Sign up

Export Citation Format

Share Document