Carbon Dot based Fluorescence sensor for Retinoic acid

2018 ◽  
Vol 3 (2) ◽  
pp. 673-677 ◽  
Author(s):  
Sristi Majumdar ◽  
Tuhin Bhattacharjee ◽  
Debajit Thakur ◽  
Devasish Chowdhury
RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57327-57334 ◽  
Author(s):  
Sristi Majumdar ◽  
Upama Baruah ◽  
Gitanjali Majumdar ◽  
Debajit Thakur ◽  
Devasish Chowdhury

In this work a paper carbon dot (PCDs) based fluorescence sensor was developed which can distinguish between the organic and inorganic sulphur in analytes.


Author(s):  
Wan Ji ◽  
Jialuo Yu ◽  
Jianxia Cheng ◽  
Longwen Fu ◽  
Zhiyang Zhang ◽  
...  

2019 ◽  
Vol 43 (30) ◽  
pp. 12137-12151 ◽  
Author(s):  
Paulami Mandal ◽  
Dibakar Sahoo ◽  
Priyatosh Sarkar ◽  
Kaushik Chakraborty ◽  
Sukhen Das

Green chemistry C-Dot-based ‘turn-on’ and ‘turn-off’ fluorescence sensor for pesticides [imidacloprid (LOD ∼ 0.013 μM) and tetradifon (LOD ∼ 0.04 μM)] in aqueous solution.


2021 ◽  
Vol 23 (1) ◽  
pp. 190
Author(s):  
Thi-Hoa Le ◽  
Ji-Hyeon Kim ◽  
Sang-Joon Park

Glutathione (GSH) is a thiol that plays a significant role in nutrient metabolism, antioxidant defense and the regulation of cellular events. GSH deficiency is related to variety of diseases, so it is useful to develop novel approaches for GSH evaluation and detection. In this study we used nitrogen and phosphorus co-doped carbon dot-gold nanoparticle (NPCD–AuNP) composites to fabricate a simple and selective fluorescence sensor for GSH detection. We employed the reductant potential of the nitrogen and phosphorus co-doped carbon dots (NPCDs) themselves to form AuNPs, and subsequently NPCD–AuNP composites from Au3+. The composites were characterized by using a range of spectroscopic and electron microscopic techniques, including electrophoretic light scattering and X-ray diffraction. The overlap of the fluorescence emission spectrum of NPCDs and the absorption spectrum of AuNPs resulted in an effective inner filter effect (IFE) in the composite material, leading to a quenching of the fluorescence intensity. In the presence of GSH, the fluorescence intensity of the composite was recovered, which increased proportionally to increasing the GSH concentration. In addition, our GSH sensing method showed good selectivity and sensing potential in human serum with a limit of detection of 0.1 µM and acceptable results.


2000 ◽  
Vol 70 (3) ◽  
pp. 92-101 ◽  
Author(s):  
Burim Ametaj ◽  
Brian Nonnecke ◽  
Ronald Horst ◽  
Donald Beitz

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.


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