Cover Picture: Single molecule fluorescence reveals dimerization of myristoylated Src N-terminal region on supported lipid bilayers (ChemistrySelect 4/2016)

2016 ◽  
Vol 1 (4) ◽  
pp. 641-641
Author(s):  
Anabel-Lise Le Roux ◽  
Bruno Castro ◽  
Erik T. Garbacik ◽  
Maria F. Garcia Parajo ◽  
Miquel Pons
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Terminal Region ◽  
Supported Lipid Bilayers ◽  
Single Molecule Fluorescence

scholarly journals Single molecule fluorescence reveals dimerization of myristoylated Src N-terminal region on supported lipid bilayers

2016 ◽  
Vol 1 (4) ◽  
pp. 642-647 ◽  
Author(s):  
Anabel-Lise Le Roux ◽  
Bruno Castro ◽  
Erik T. Garbacik ◽  
Maria F. Garcia Parajo ◽  
Miquel Pons
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Terminal Region ◽  
Supported Lipid Bilayers ◽  
Single Molecule Fluorescence

Single-Molecule Fluorescence Imaging of Peptide Binding to Supported Lipid Bilayers

2009 ◽  
Vol 81 (13) ◽  
pp. 5130-5138 ◽  
Author(s):  
Christopher B. Fox ◽  
Joshua R. Wayment ◽  
Grant A. Myers ◽  
Scott K. Endicott ◽  
Joel M. Harris
Keyword(s):  
Fluorescence Imaging ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Peptide Binding ◽  
Supported Lipid Bilayers ◽  
Single Molecule Fluorescence

scholarly journals Correlated diffusion in lipid bilayers

2021 ◽  
Vol 118 (48) ◽  
pp. e2113202118
Author(s):  
Rafael L. Schoch ◽  
Frank L. H. Brown ◽  
Gilad Haran
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Lipid Membranes ◽  
Supported Lipid Bilayers ◽  
Single Molecule Tracking ◽  
Black Lipid Membranes ◽  
Molecular Tracking ◽  
Physical Materials ◽  
Multiple Molecules ◽  
Major Target

Lipid membranes are complex quasi–two-dimensional fluids, whose importance in biology and unique physical/materials properties have made them a major target for biophysical research. Recent single-molecule tracking experiments in membranes have caused some controversy, calling the venerable Saffman–Delbrück model into question and suggesting that, perhaps, current understanding of membrane hydrodynamics is imperfect. However, single-molecule tracking is not well suited to resolving the details of hydrodynamic flows; observations involving correlations between multiple molecules are superior for this purpose. Here dual-color molecular tracking with submillisecond time resolution and submicron spatial resolution is employed to reveal correlations in the Brownian motion of pairs of fluorescently labeled lipids in membranes. These correlations extend hundreds of nanometers in freely floating bilayers (black lipid membranes) but are severely suppressed in supported lipid bilayers. The measurements are consistent with hydrodynamic predictions based on an extended Saffman–Delbrück theory that explicitly accounts for the two-leaflet bilayer structure of lipid membranes.

scholarly journals HDL particles incorporate into lipid bilayers – a combined AFM and single molecule fluorescence microscopy study

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Birgit Plochberger ◽  
Clemens Röhrl ◽  
Johannes Preiner ◽  
Christian Rankl ◽  
Mario Brameshuber ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Microscopy Study ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fluorescence Microscopy

Single-Molecule Detection with Lightguiding Nanowires: Determination of Protein Concentration and Diffusivity in Supported Lipid Bilayers

Nano Letters ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 6182-6191 ◽  
Author(s):  
Damiano Verardo ◽  
Björn Agnarsson ◽  
Vladimir P. Zhdanov ◽  
Fredrik Höök ◽  
Heiner Linke
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Protein Concentration ◽  
Single Molecule Detection ◽  
Supported Lipid Bilayers

scholarly journals Single-molecule fluorescence vistas of how lipids regulate membrane proteins

2021 ◽  
Author(s):  
Alyssa E. Ward ◽  
Yujie Ye ◽  
Jennifer A. Schuster ◽  
Shushu Wei ◽  
Francisco N. Barrera
Keyword(s):  
Membrane Proteins ◽  
Physical Properties ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Short Review ◽  
Single Molecule Fluorescence ◽  
Determining Factors ◽  
Fluorescence Methods ◽  
Basic Understanding ◽  
Sample Heterogeneity

The study of membrane proteins is undergoing a golden era, and we are gaining unprecedented knowledge on how this key group of proteins works. However, we still have only a basic understanding of how the chemical composition and the physical properties of lipid bilayers control the activity of membrane proteins. Single-molecule (SM) fluorescence methods can resolve sample heterogeneity, allowing to discriminate between the different molecular populations that biological systems often adopt. This short review highlights relevant examples of how SM fluorescence methodologies can illuminate the different ways in which lipids regulate the activity of membrane proteins. These studies are not limited to lipid molecules acting as ligands, but also consider how the physical properties of the bilayer can be determining factors on how membrane proteins function.

scholarly journals Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity

2016 ◽  
Vol 113 (46) ◽  
pp. E7185-E7193 ◽  
Author(s):  
Rahul Grover ◽  
Janine Fischer ◽  
Friedrich W. Schwarz ◽  
Wilhelm J. Walter ◽  
Petra Schwille ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Gliding Motility ◽  
Supported Lipid Bilayers ◽  
Transport Efficiency ◽  
Collective Transport ◽  
Wide Range ◽  
Motor Density

In eukaryotic cells, membranous vesicles and organelles are transported by ensembles of motor proteins. These motors, such as kinesin-1, have been well characterized in vitro as single molecules or as ensembles rigidly attached to nonbiological substrates. However, the collective transport by membrane-anchored motors, that is, motors attached to a fluid lipid bilayer, is poorly understood. Here, we investigate the influence of motors’ anchorage to a lipid bilayer on the collective transport characteristics. We reconstituted “membrane-anchored” gliding motility assays using truncated kinesin-1 motors with a streptavidin-binding peptide tag that can attach to streptavidin-loaded, supported lipid bilayers. We found that the diffusing kinesin-1 motors propelled the microtubules in the presence of ATP. Notably, we found the gliding velocity of the microtubules to be strongly dependent on the number of motors and their diffusivity in the lipid bilayer. The microtubule gliding velocity increased with increasing motor density and membrane viscosity, reaching up to the stepping velocity of single motors. This finding is in contrast to conventional gliding motility assays where the density of surface-immobilized kinesin-1 motors does not influence the microtubule velocity over a wide range. We reason that the transport efficiency of membrane-anchored motors is reduced because of their slippage in the lipid bilayer, an effect that we directly observed using single-molecule fluorescence microscopy. Our results illustrate the importance of motor–cargo coupling, which potentially provides cells with an additional means of regulating the efficiency of cargo transport.

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