Surface characterization of bone graft substitute materials conditioned in cell culture medium

2010 ◽  
Vol 42 (6-7) ◽  
pp. 452-456 ◽  
Author(s):  
Z. Mladenovic ◽  
A. Sahlin-Platt ◽  
Å. Bengtsson ◽  
M. Ransjö ◽  
A. Shchukarev
Keyword(s):  
Cell Culture ◽  
Bone Graft ◽  
Surface Characterization ◽  
Culture Medium ◽  
Bone Graft Substitute ◽  
Cell Culture Medium ◽  
Substitute Materials

scholarly journals Surface Characterization of Amorphous Zr-Al-(Ni, Cu) Alloys Immersed in Cell-Culture Medium

2002 ◽  
Vol 43 (2) ◽  
pp. 261-266 ◽  
Author(s):  
Sachiko Hiromoto ◽  
Katsuhiko Asami ◽  
An Pang Tsai ◽  
Takao Hanawa
Keyword(s):  
Cell Culture ◽  
Surface Characterization ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Cu Alloys

Surface characterization of insulin-coated Ti6Al4V medical implants conditioned in cell culture medium: An XPS study

2017 ◽  
Vol 216 ◽  
pp. 33-38
Author(s):  
Andrey Shchukarev ◽  
Behnosh Öhrnell Malekzadeh ◽  
Maria Ransjö ◽  
Pentti Tengvall ◽  
Anna Westerlund
Keyword(s):  
Cell Culture ◽  
Surface Characterization ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Medical Implants ◽  
Xps Study

Characterization of fullerene colloidal suspension in a cell culture medium for in vitro toxicity assessment

2010 ◽  
Vol 6 (7) ◽  
pp. 1238 ◽  
Author(s):  
Haruhisa Kato ◽  
Naohide Shinohara ◽  
Ayako Nakamura ◽  
Masanori Horie ◽  
Katsuhide Fujita ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Colloidal Suspension ◽  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Cell Culture Medium ◽  
A Cell ◽  
In Vitro Toxicity Assessment

scholarly journals Purification and characterization of testicular transferrin secreted by rat Sertoli cells

1984 ◽  
Vol 218 (2) ◽  
pp. 313-320 ◽  
Author(s):  
M K Skinner ◽  
W L Cosand ◽  
M D Griswold
Keyword(s):  
Cell Culture ◽  
Sertoli Cells ◽  
Culture Medium ◽  
Serum Transferrin ◽  
Cell Culture Medium ◽  
Purification And Characterization ◽  
Reverse Phase ◽  
Rat Serum ◽  
Molecular Masses

Sertoli cells synthesize and secrete a transferrin-like protein (testicular transferrin) [Skinner & Griswold (1980) J. Biol. Chem. 255, 1923-1925]. The purpose of the present study was to purify and characterize testicular transferrin and to compare it with serum transferrin. Testicular transferrin was obtained from the medium of cultured rat Sertoli cells, whereas serum transferrin was obtained from rat serum. Both proteins were purified with the use of phenyl-Sepharose hydrophobic chromatography and transferrin immunoaffinity chromatography. The purified proteins were shown to have similar molecular masses (75 000 Da) and amino acid compositions. The pattern of tryptic peptides from testicular and serum transferrin were found to be essentially the same when analysed by reverse-phase high-pressure liquid chromatography. The carbohydrate composition of both transferrins was determined by several colorimetric assays and g.l.c. Testicular transferrin, isolated from cell culture medium, had increased amounts of glucose, galactose and glucosamine. Serum transferrin that was incubated with cell culture medium also had a large amount of associated glucose. The results show that testicular transferrin and serum transferrin are structurally very similar and are possibly products of the same gene expressed in two different tissues, the testis and liver. However, the amount of carbohydrate associated with these two proteins is different.

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