scholarly journals Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes

2019 ◽  
Vol 8 (6) ◽  
pp. 593-605 ◽  
Author(s):  
Yu Zhu ◽  
Yachao Jia ◽  
Yanmao Wang ◽  
Jia Xu ◽  
Yimin Chai
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Regenerative Effect ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Immunomodulatory Function of Bone Marrow-Derived Mesenchymal Stem Cells in Experimental Autoimmune Type 1 Diabetes

2009 ◽  
Vol 183 (2) ◽  
pp. 993-1004 ◽  
Author(s):  
Paolo Fiorina ◽  
Mollie Jurewicz ◽  
Andrea Augello ◽  
Andrea Vergani ◽  
Shirine Dada ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Type 1 Diabetes ◽  
Mesenchymal Stem Cells ◽  
Immunomodulatory Function ◽  
Experimental Autoimmune

scholarly journals Tendon and Cytokine Marker Expression by Human Bone Marrow Mesenchymal Stem Cells in a Hyaluronate/Poly-Lactic-Co-Glycolic Acid (PLGA)/Fibrin Three-Dimensional (3D) Scaffold

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1268 ◽  
Author(s):  
Maria C. Ciardulli ◽  
Luigi Marino ◽  
Joseph Lovecchio ◽  
Emanuele Giordano ◽  
Nicholas R. Forsyth ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Glycolic Acid ◽  
Three Dimensional ◽  
Human Bone ◽  
3D Scaffold ◽  
Marrow Mesenchymal Stem Cells ◽  
Tgf Β1

We developed a (three-dimensional) 3D scaffold, we named HY-FIB, incorporating a force-transmission band of braided hyaluronate embedded in a cell localizing fibrin hydrogel and poly-lactic-co-glycolic acid (PLGA) nanocarriers as transient components for growth factor controlled delivery. The tenogenic supporting capacity of HY-FIB on human-Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) was explored under static conditions and under bioreactor-induced cyclic strain conditions. HY-FIB elasticity enabled to deliver a mean shear stress of 0.09 Pa for 4 h/day. Tendon and cytokine marker expression by hBM-MSCs were studied. Results: hBM-MSCs embedded in HY-FIB and subjected to mechanical stimulation, resulted in a typical tenogenic phenotype, as indicated by type 1 Collagen fiber immunofluorescence. RT-qPCR showed an increase of type 1 Collagen, scleraxis, and decorin gene expression (3-fold, 1600-fold, and 3-fold, respectively, at day 11) in dynamic conditions. Cells also showed pro-inflammatory (IL-6, TNF, IL-12A, IL-1β) and anti-inflammatory (IL-10, TGF-β1) cytokine gene expressions, with a significant increase of anti-inflammatory cytokines in dynamic conditions (IL-10 and TGF-β1 300-fold and 4-fold, respectively, at day 11). Mechanical signaling, conveyed by HY-FIB to hBM-MSCs, promoted tenogenic gene markers expression and a pro-repair cytokine balance. The results provide strong evidence in support of the HY-FIB system and its interaction with cells and its potential for use as a predictive in vitro model.

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