scholarly journals Guiding Self-Assembly with the Tip of an Atomic Force Microscope

Scanning ◽  
2006 ◽  
Vol 24 (3) ◽  
pp. 117-120 ◽  
Author(s):  
Patrick Mesquida ◽  
Andreas Stemmer
Keyword(s):  
Atomic Force Microscope ◽  
Self Assembly ◽  
Atomic Force

Hydrogen-Bonding Layer Protecting Polyelectrolyte Capsules and Its Mechanical Property Study with Atomic Force Microscope

2008 ◽  
Vol 8 (6) ◽  
pp. 2996-3002 ◽  
Author(s):  
Liqin Ge ◽  
Xing Wang ◽  
Long Ba ◽  
Zhongze Gu
Keyword(s):  
Hydrogen Bonding ◽  
Atomic Force Microscope ◽  
Elastic Deformation ◽  
Self Assembly ◽  
Laser Scanning ◽  
Confocal Laser ◽  
Bonding Layer ◽  
Layer By Layer ◽  
Assembly Method ◽  
Atomic Force

The hydrogen-bonding multilayered polyelectrolyte capsules with sizes around 6 μm were fabricated by layer-by-layer self-assembly method. The morphology of the obtained capsules was observed with Scanning Electron Microscope (SEM), Confocal Laser Scanning Microscope (CLSM) and Atomic Force Microscope (AFM), respectively. The elastic properties of the capsules were studied with AFM. The capsule was pressed by cantilever with different lengths, a glass bead glued at the end of the cantilever. The force curves were measured on the capsule in air. The Young's modulus of the capsule was obtained (E = 170 MPa for the loading). Results show that this model can predict the elastic deformation of the microcapsule. The accuracy of the elastic deformation of polymer capsule can be ensured using a cantilever of mediate stiffness. Our results show that the existence of the hydrogen-bonding layer makes the multilayered polyelectrolyte harder in comparison with the pure multilayered polyelectrolyte capsules.

Atomic force microscope characterization of self-assembly behaviors of cyclo[8] pyrrole on solid substrates

2017 ◽  
Vol 674 ◽  
pp. 151-156 ◽  
Author(s):  
Hai Xu ◽  
Siqi Zhao ◽  
Xiang Xiong ◽  
Jinzhi Jiang ◽  
Wei Xu ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
Self Assembly ◽  
Atomic Force ◽  
Solid Substrates

Influence of Tunable External Stimuli on the Self-Assembly of Guanosine Supramolecular Nanostructures Studied By Atomic Force Microscope

Langmuir ◽  
2009 ◽  
Vol 25 (23) ◽  
pp. 13432-13437 ◽  
Author(s):  
Yinli Li ◽  
Mingdong Dong ◽  
Daniel E Otzen ◽  
Yuheng Yao ◽  
Bo Liu ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
Self Assembly ◽  
The Self ◽  
Atomic Force ◽  
External Stimuli

Characterization of photosystem II with the atomic-force microscope

1992 ◽  
Vol 50 (2) ◽  
pp. 1146-1147
Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon
Keyword(s):  
Photosystem Ii ◽  
Atomic Force Microscope ◽  
Three Dimensional ◽  
Biologically Active ◽  
Atomic Force ◽  
Freeze Etching ◽  
Image Averaging ◽  
Oxygen Evolving ◽  
Averaging Techniques

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.

Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

1989 ◽  
Vol 47 ◽  
pp. 32-33
Author(s):  
S.A.C. Gould ◽  
B. Drake ◽  
C.B. Prater ◽  
A.L. Weisenhorn ◽  
S.M. Lindsay ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Aqueous Solutions ◽  
Atomic Force Microscope ◽  
Piezoelectric Crystal ◽  
Atomic Force ◽  
Structure Of Molecules ◽  
Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

The atomic-force microscope and its future in biology

1993 ◽  
Vol 51 ◽  
pp. 704-705
Author(s):  
Jean-Paul Revel
Keyword(s):  
Silicon Nitride ◽  
Laser Beam ◽  
Atomic Force Microscope ◽  
Scanning Tunneling Microscope ◽  
Point Of View ◽  
Scanning Tunneling ◽  
Close Relative ◽  
Tunneling Microscope ◽  
Atomic Force ◽  
Sharp Point

The last few years have been marked by a series of remarkable developments in microscopy. Perhaps the most amazing of these is the growth of microscopies which use devices where the place of the lens has been taken by probes, which record information about the sample and display it in a spatial from the point of view of the context. From the point of view of the biologist one of the most promising of these microscopies without lenses is the scanned force microscope, aka atomic force microscope.This instrument was invented by Binnig, Quate and Gerber and is a close relative of the scanning tunneling microscope. Today's AFMs consist of a cantilever which bears a sharp point at its end. Often this is a silicon nitride pyramid, but there are many variations, the object of which is to make the tip sharper. A laser beam is directed at the back of the cantilever and is reflected into a split, or quadrant photodiode.

Sign in / Sign up

Export Citation Format

Share Document