scholarly journals Imaging of intramembranous particles in frozen-hydrated cells (Saccharomyces cerevisiae ) by high resolution cryo SEM

Scanning ◽  
1990 ◽  
Vol 12 (6) ◽  
pp. 300-307 ◽  
Author(s):  
P. Walther ◽  
J. Hentschel ◽  
P. Herter ◽  
T. Müller ◽  
K. Zierold
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Resolution ◽  
Intramembranous Particles

scholarly journals High-Resolution Genome-Wide Occupancy in Candida spp. Using ChEC-seq

mSphere ◽  
2020 ◽  
Vol 5 (5) ◽  
Author(s):  
Faiza Tebbji ◽  
Inès Khemiri ◽  
Adnane Sellam
Keyword(s):  
Public Health ◽  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
High Resolution ◽  
Transcriptional Regulator ◽  
Candida Auris ◽  
Content Type ◽  
Genome Wide ◽  
Public Health Threat

ABSTRACT To persist in their dynamic human host environments, fungal pathogens must sense and adapt by modulating their gene expression to fulfill their cellular needs. Understanding transcriptional regulation on a global scale would uncover cellular processes linked to persistence and virulence mechanisms that could be targeted for antifungal therapeutics. Infections associated with the yeast Candida albicans, a highly prevalent fungal pathogen, and the multiresistant related species Candida auris are becoming a serious public health threat. To define the set of a gene regulated by a transcriptional regulator in C. albicans, chromatin immunoprecipitation (ChIP)-based techniques, including ChIP with microarray technology (ChIP-chip) or ChIP-DNA sequencing (ChIP-seq), have been widely used. Here, we describe a new set of PCR-based micrococcal nuclease (MNase)-tagging plasmids for C. albicans and other Candida spp. to determine the genome-wide location of any transcriptional regulator of interest using chromatin endogenous cleavage (ChEC) coupled to high-throughput sequencing (ChEC-seq). The ChEC procedure does not require protein-DNA cross-linking or sonication, thus avoiding artifacts related to epitope masking or the hyper-ChIPable euchromatic phenomenon. In a proof-of-concept application of ChEC-seq, we provided a high-resolution binding map of the SWI/SNF chromatin remodeling complex, a master regulator of fungal fitness in C. albicans, in addition to the transcription factor Nsi1 that is an ortholog of the DNA-binding protein Reb1 for which genome-wide occupancy was previously established in Saccharomyces cerevisiae. The ChEC-seq procedure described here will allow a high-resolution genomic location definition which will enable a better understanding of transcriptional regulatory circuits that govern fungal fitness and drug resistance in these medically important fungi. IMPORTANCE Systemic fungal infections caused by Candida albicans and the “superbug” Candida auris are becoming a serious public health threat. The ability of these yeasts to cause disease is linked to their faculty to modulate the expression of genes that mediate their escape from the immune surveillance and their persistence in the different unfavorable niches within the host. Comprehensive knowledge on gene expression control of fungal fitness is consequently an interesting framework for the identification of essential infection processes that could be hindered by chemicals as potential therapeutics. Here, we expanded the use of ChEC-seq, a technique that was initially developed in the yeast model Saccharomyces cerevisiae to identify genes that are modulated by a transcriptional regulator, in pathogenic yeasts from the genus Candida. This robust technique will allow a better characterization of key gene expression regulators and their contribution to virulence and antifungal resistance in these pathogenic yeasts.

scholarly journals Crystal structures of Hsp104 N-terminal domains fromSaccharomyces cerevisiaeandCandida albicanssuggest the mechanism for the function of Hsp104 in dissolving prions

2017 ◽  
Vol 73 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Peng Wang ◽  
Jingzhi Li ◽  
Clarissa Weaver ◽  
Aaron Lucius ◽  
Bingdong Sha
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
High Resolution ◽  
Crystal Structures ◽  
Molecular Chaperone ◽  
Peptide Binding ◽  
Dimer Formation ◽  
Peptide Substrates ◽  
Peptide Binding Groove ◽  
Binding Groove

Hsp104 is a yeast member of the Hsp100 family which functions as a molecular chaperone to disaggregate misfolded polypeptides. To understand the mechanism by which the Hsp104 N-terminal domain (NTD) interacts with its peptide substrates, crystal structures of the Hsp104 NTDs fromSaccharomyces cerevisiae(ScHsp104NTD) andCandida albicans(CaHsp104NTD) have been determined at high resolution. The structures of ScHsp104NTD and CaHsp104NTD reveal that the yeast Hsp104 NTD may utilize a conserved putative peptide-binding groove to interact with misfolded polypeptides. In the crystal structures ScHsp104NTD forms a homodimer, while CaHsp104NTD exists as a monomer. The consecutive residues Gln105, Gln106 and Lys107, and Lys141 around the putative peptide-binding groove mediate the monomer–monomer interactions within the ScHsp104NTD homodimer. Dimer formation by ScHsp104NTD suggests that the Hsp104 NTD may specifically interact with polyQ regions of prion-prone proteins. The data may reveal the mechanism by which Hsp104 NTD functions to suppress and/or dissolve prions.

scholarly journals High-Resolution Genetic Mapping With Ordered Arrays of Saccharomyces cerevisiae Deletion Mutants

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1091-1099 ◽  
Author(s):  
Paul Jorgensen ◽  
Bryce Nelson ◽  
Mark D Robinson ◽  
Yiqun Chen ◽  
Brenda Andrews ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Resolution ◽  
Genetic Mapping ◽  
Large Scale ◽  
Model Organisms ◽  
Deletion Mutants ◽  
Wild Type ◽  
Allele Specific ◽  
Single Allele ◽  
Kinase Signaling Pathway

Abstract We present a method for high-resolution genetic mapping that takes advantage of the ordered set of viable gene deletion mutants, which form a set of colinear markers covering almost every centimorgan of the Saccharomyces cerevisiae genome, and of the synthetic genetic array (SGA) system, which automates the construction of double mutants formed by mating and meiotic recombination. The Cbk1 kinase signaling pathway, which consists minimally of CBK1, MOB2, KIC1, HYM1, and TAO3 (PAG1), controls polarized morphogenesis and activation of the Ace2 transcription factor. Deletion mutations in the Cbk1 pathway genes are tolerated differently by common laboratory strains of S. cerevisiae, being viable in the W303 background but dead in the S288C background. Genetic analysis indicated that the lethality of Cbk1 pathway deletions in the S288C background was suppressed by a single allele specific to the W303 background. SGA mapping (SGAM) was used to locate this W303-specific suppressor to the SSD1 locus, which contains a known polymorphism that appears to compromise SSD1 function. This procedure should map any mutation, dominant or recessive, whose phenotype is epistatic to wild type, that is, a phenotype that can be scored from a mixed population of cells obtained by germination of both mutant and wild-type spores. In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach.

scholarly journals High-resolution analysis of condition-specific regulatory modules in Saccharomyces cerevisiae

2008 ◽  
Vol 9 (1) ◽  
pp. R2 ◽  
Author(s):  
Hun-Goo Lee ◽  
Hyo-Soo Lee ◽  
Sang-Hoon Jeon ◽  
Tae-Hoon Chung ◽  
Young-Sung Lim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Resolution ◽  
Regulatory Modules ◽  
High Resolution Analysis ◽  
Resolution Analysis

Genetic pleiotropy in Saccharomyces cerevisiae quantified by high-resolution phenotypic profiling

2006 ◽  
Vol 275 (6) ◽  
pp. 605-614 ◽  
Author(s):  
Elke Ericson ◽  
Ilona Pylvänäinen ◽  
Luciano Fernandez-Ricaud ◽  
Olle Nerman ◽  
Jonas Warringer ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Resolution ◽  
Genetic Pleiotropy
Sign in / Sign up

Export Citation Format

Share Document