scholarly journals Scanning slit aperture confocal microscopy for three-dimensional imaging

Scanning ◽  
1990 ◽  
Vol 12 (3) ◽  
pp. 156-160 ◽  
Author(s):  
D. H. Burns ◽  
R. B. Hatangadi ◽  
F. A. Spelman
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging

scholarly journals Three-dimensional imaging with reflection synthetic confocal microscopy

2020 ◽  
Vol 45 (13) ◽  
pp. 3721
Author(s):  
Md Rasedujjaman ◽  
Kévin Affannoukoué ◽  
Nicolas Garcia-Seyda ◽  
Philippe Robert ◽  
Hugues Giovannini ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging

Clearing, immunofluorescence, and confocal microscopy for the three-dimensional imaging of murine testes and study of testis biology

2020 ◽  
Vol 209 (3) ◽  
pp. 107449
Author(s):  
Jason A. Kaufman ◽  
Monica J. Castro ◽  
Saul A. Ruiz ◽  
Garilyn M. Jentarra ◽  
Bernardo Chavira ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging

scholarly journals Confocal microscopy on the beamline: novel three-dimensional imaging and sample positioning

2012 ◽  
Vol 45 (5) ◽  
pp. 936-943 ◽  
Author(s):  
I. Khan ◽  
R. Gillilan ◽  
I. Kriksunov ◽  
R. Williams ◽  
W. R. Zipfel ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Second Harmonic ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Protein Crystal ◽  
Protein Crystals ◽  
Wide Field ◽  
Three Dimensional Imaging ◽  
Biological Studies ◽  
Confocal Optics

Confocal microscopy, a technique that has been extensively applied in cellular biological studies, may also be applied to the visualization and three-dimensional imaging of protein crystals at high resolution on synchrotron beamlines. Protein crystal samples are examined using a commercially available confocal microscope adapted for cryogenic use. A preliminary test using a custom confocal design adapted for beamline use is also presented. The confocal optics configuration is compatible with nonlinear imaging techniques such as two-photon excited fluorescence imaging and second harmonic generation. The possibilities of this method are explored using two modes: fluorescence and reflection confocal. In fluorescence mode, small amounts of dye are introduced into the crystal through soaking or growth conditions. Under such conditions, protein crystals are easily resolved from salts and amorphous precipitates, which do not generally take up dye. Reflection mode, which does not require dye, still exhibits greater resolution and sensitivity to surface detail than conventional wide-field microscopy as a result of the confocal optics configuration. The inherent three-dimensional nature of the method means that on-axis sample views (along the direction of the X-ray beam) can be reconstructed from an off-axis configuration, simplifying the beamline setup and providing uniquely detailed views of cryogenically cooled crystals.

CSI Helsinki: comparing three-dimensional imaging of diagonal cutter toolmarks using confocal microscopy and SWLI

2010 ◽  
Author(s):  
I. Kassamakov ◽  
C. Barbeau ◽  
S. Lehto ◽  
P. Ahvenainen ◽  
T. Reinikainen ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging
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