scholarly journals The mechanism of kaempferol induced apoptosis and inhibited proliferation in human cervical cancer SiHa cell: From macro to nano

Scanning ◽  
2016 ◽  
Vol 38 (6) ◽  
pp. 644-653 ◽  
Author(s):  
Lv-Ying Tu ◽  
Hai-Hua Bai ◽  
Ji-Ye Cai ◽  
Sui-Ping Deng
Keyword(s):  
Cervical Cancer ◽  
Siha Cell ◽  
Induced Apoptosis ◽  
Human Cervical Cancer

scholarly journals Hsp90 Inhibitor SNX-2112 Enhances TRAIL-Induced Apoptosis of Human Cervical Cancer Cells via the ROS-Mediated JNK-p53-Autophagy-DR5 Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-26
Author(s):  
Liubing Hu ◽  
Yan Wang ◽  
Zui Chen ◽  
Liangshun Fu ◽  
Sheng Wang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Hela Cells ◽  
Cell Apoptosis ◽  
Hsp90 Inhibitor ◽  
Cervical Cancer Cells ◽  
Ros Scavenger ◽  
Induced Apoptosis ◽  
Human Cervical Cancer

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent cancer cell apoptosis-inducing factor that can induce apoptosis in a variety of cancer cells. However, resistance to TRAIL in cancer cells is a huge obstacle in creating effective TRAIL-targeted clinical therapies. Thus, agents that can either enhance the effect of TRAIL or overcome its resistance are needed. In this study, we combined TRAIL with SNX-2112, an Hsp90 inhibitor we previously developed, to explore the effect and mechanism that SNX-2112 enhanced TRAIL-induced apoptosis in cervical cancer cells. Our results showed that SNX-2112 markedly enhanced TRAIL-induced cytotoxicity in HeLa cells, and this combination was found to be synergistic. Additionally, we found that SNX-2112 sensitized TRAIL-mediated apoptosis caspase-dependently in TRAIL-resistant HeLa cells. Mechanismly, SNX-2112 downregulated antiapoptosis proteins, including Bcl-2, Bcl-XL, and FLIP, promoted the accumulation of reactive oxygen species (ROS), and increased the expression levels of p-JNK and p53. ROS scavenger NAC rescued SNX-2112/TRAIL-induced apoptosis and suppressed SNX-2112-induced p-JNK and p53. Moreover, SNX-2112 induced the upregulation of death-receptor DR5 in HeLa cells. The silencing of DR5 by siRNA significantly decreased cell apoptosis by the combined effect of SNX-2112 and TRAIL. In addition, SNX-2112 inhibited the Akt/mTOR signaling pathway and induced autophagy in HeLa cells. The blockage of autophagy by bafilomycin A1 or Atg7 siRNA abolished SNX-2112-induced upregulation of DR5. Meanwhile, ROS scavenger NAC, JNK inhibitor SP600125, and p53 inhibitor PFTα were used to verify that autophagy-mediated upregulation of DR5 was regulated by the SNX-2112-stimulated activation of the ROS-JNK-p53 signaling pathway. Thus, the combination of SNX-2112 and TRAIL may provide a novel strategy for the treatment of human cervical cancer by overcoming cellular mechanisms of apoptosis resistance.

scholarly journals Involvement of mitochondria and caspase pathways in N-demethyl-clarithromycin-induced apoptosis in human cervical cancer HeLa cell

2006 ◽  
Vol 27 (12) ◽  
pp. 1622-1629 ◽  
Author(s):  
Ai-min QIAO ◽  
Takashi IKEJIMA ◽  
Shin-ichi TASHIRO ◽  
Satoshi ONODERA ◽  
Wei-ge ZHANG ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Induced Apoptosis ◽  
Human Cervical Cancer

Mechanism of ricin-induced apoptosis in human cervical cancer cells

2005 ◽  
Vol 69 (5) ◽  
pp. 855-865 ◽  
Author(s):  
P.V. Lakshmana Rao ◽  
R. Jayaraj ◽  
A.S.B. Bhaskar ◽  
Om Kumar ◽  
R. Bhattacharya ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cervical Cancer Cells ◽  
Induced Apoptosis ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

scholarly journals Proteomic Investigation into Betulinic Acid-Induced Apoptosis of Human Cervical Cancer HeLa Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105768 ◽  
Author(s):  
Tao Xu ◽  
Qiuying Pang ◽  
Dong Zhou ◽  
Aiqin Zhang ◽  
Shaman Luo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Betulinic Acid ◽  
Proteomic Investigation ◽  
Induced Apoptosis ◽  
Human Cervical Cancer

Pinelliae Rhizoma Herbal-Acupuncture Solution Induced Apoptosis in Human Cervical Cancer Cells, SNU-17

2006 ◽  
Vol 34 (03) ◽  
pp. 401-408 ◽  
Author(s):  
Jong-Seok Yoon ◽  
Jung-Chul Seo ◽  
Sang-Won Han
Keyword(s):  
Cervical Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Caspase 3 ◽  
Dapi Staining ◽  
Apoptotic Gene ◽  
Cervical Cancer Cells ◽  
Induced Apoptosis ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

Pinelliae Rhizoma has been used traditionally in Korea to promote the liver Qi activity and the function of the digestive system. We investigated whether the Pinelliae Rhizoma herbal-acupuncture solution (PRHS) would induce cell-death on SNU-17, human cervical cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to investigate the cytotoxicity of PRHS. The cell death was identified as apoptosis with 4, 6-diamidineo-2-phenylindole (DAPI) staining, and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. PRHS could induce apoptosis of SNU-17 via Bax-related caspase-3 activation. The expressions of both Bax, a pro-apoptotic gene, and caspase-3, an apoptotic gene, were increased. The results might provide the experimental data for the clinical use of Pinelliae Rhizoma on cervical cancer.

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