scholarly journals A novel experimental study on the fabrication and biological characteristics of canine bone marrow mesenchymal stem cells sheet using vitamin C

Scanning ◽  
2015 ◽  
Vol 37 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Peng Guo ◽  
Jing-jing Zeng ◽  
Nuo Zhou
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Experimental Study ◽  
Mesenchymal Stem Cells ◽  
Vitamin C ◽  
Biological Characteristics ◽  
Marrow Mesenchymal Stem Cells

Effect of miR-124 on the Biological Characteristics of Bone Marrow Mesenchymal Stem Cells in Tumor Microenvironment

2020 ◽  
Vol 10 (2) ◽  
pp. 151-156
Author(s):  
Linfu He ◽  
XiaoyanHe ◽  
Xiaocui Liu ◽  
Wenjing Shi ◽  
PeiXu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Tumor Microenvironment ◽  
Glioma Cells ◽  
Biological Characteristics ◽  
Brain Glioma ◽  
Marrow Mesenchymal Stem Cells

miR-124 affects migration and differentiation of mesenchymal stem cells (MSCs), but its role in bone marrow mesenchymal stem cells (BMSCs) in tumor microenvironment remain unclear. Therefore, our study aims to assess miR-124’s role in BMSCs in glioma microenvironment. BMSCs were isolated and co-cultured with glioma cells using Transwell chamber and then transfected with miR-124 mimic/NC followed by analysis of BMSCs biological characteristics (cell proliferation by CCK8, miR-124 level by Real-time PCR, cell cycle and apoptosis by flow cytometry, and cell migration by Transwell). There were significant differences of cell proliferation, migration, apoptosis and cell cycle in BMSCs group compared to co-culture and co-culture+ miR-NC group (P < 0.05), without no difference compared to co-culture + miR-124 group. In addition, co-culture + miR-124 also showed significant differences of BMSCs' biological characteristics (P < 0.05). miR-124 can alter the biological characteristics of BMSCs in the tumor microenvironment simulated by C6 brain glioma cells.

Sign in / Sign up

Export Citation Format

Share Document