scholarly journals Adrenaline enhances in vitro platelet activation and aggregation in blood samples from ticagrelor-treated patients

2018 ◽  
Vol 2 (4) ◽  
pp. 718-725 ◽  
Author(s):  
Sukhi Singh ◽  
Carl Johan Malm ◽  
Sofia Ramström ◽  
Camilla Hesse ◽  
Anders Jeppsson
Keyword(s):  
Platelet Activation ◽  
Blood Samples ◽  
In Vitro Platelet Activation

Methicillin-Resistant Staphylococcus aureus Induces In Vitro Platelet Activation and a Procoagulant Respons

CHEST Journal ◽  
2011 ◽  
Vol 140 (4) ◽  
pp. 991A
Author(s):  
Zechariah Franks ◽  
Robert Campbell ◽  
Andy Weyrich ◽  
Guy Zimmerman ◽  
Matthew Rondina
Keyword(s):  
Staphylococcus Aureus ◽  
Platelet Activation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
In Vitro Platelet Activation

Inhibitory effects of lycopene on in vitro platelet activation and in vivo prevention of thrombus formation

2005 ◽  
Vol 146 (4) ◽  
pp. 216-226 ◽  
Author(s):  
George Hsiao ◽  
Ying Wang ◽  
Nien-Hsuan Tzu ◽  
Tsorng-Hang Fong ◽  
Ming-Yi Shen ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Thrombus Formation ◽  
Inhibitory Effects ◽  
In Vitro Platelet Activation

Lack of effect of isradipine on in vitro platelet activation

1993 ◽  
Vol 72 (4) ◽  
pp. 373-375
Author(s):  
Julio B Fernandes ◽  
Ulhas P Naik ◽  
Mariana S Markell ◽  
Elizabeth Kornecki
Keyword(s):  
Platelet Activation ◽  
In Vitro Platelet Activation

scholarly journals Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1915 ◽  
Author(s):  
Marion Mussbacher ◽  
Teresa L. Krammer ◽  
Stefan Heber ◽  
Waltraud C. Schrottmaier ◽  
Stephan Zeibig ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Room Temperature ◽  
Ethylenediaminetetraacetic Acid ◽  
Plasma Concentrations ◽  
Circulating Microrna ◽  
Blood Samples ◽  
Edta Plasma ◽  
Thrombotic Diseases ◽  
And Storage

Blood-derived microRNA signatures have emerged as powerful biomarkers for predicting and diagnosing cardiovascular disease, cancer, and metabolic disorders. Platelets and platelet-derived microvesicles are a major source of microRNAs. We have previously shown that the inappropriate anticoagulation and storage of blood samples causes substantial platelet activation that is associated with the release of platelet-stored molecules into the plasma. However, it is currently unclear if circulating microRNA levels are affected by artificial platelet activation due to suboptimal plasma preparation. To address this issue, we used a standardized RT-qPCR test for 12 microRNAs (thrombomiR®, TAmiRNA GmbH, Vienna, Austria) that have been associated with cardiovascular and thrombotic diseases and were detected in platelets and/other hematopoietic cells. Blood was prevented from coagulating with citrate–theophylline–adenosine–dipyridamole (CTAD), sodium citrate, or ethylenediaminetetraacetic acid (EDTA) and stored for different time periods either at room temperature or at 4 °C prior to plasma preparation and the subsequent quantification of microRNAs. We found that five microRNAs (miR-191-5p, miR-320a, miR-21-5p, miR-23a-3p, and miR-451a) were significantly increased in the EDTA plasma. Moreover, we observed a time-dependent increase in plasma microRNAs that was most pronounced in the EDTA blood stored at room temperature for 24 h. Furthermore, significant correlations between microRNA levels and plasma concentrations of platelet-stored molecules pointed towards in vitro platelet activation. Therefore, we strongly recommend to (i) use CTAD as an anticoagulant, (ii) process blood samples as quickly as possible, and (iii) store blood samples at 4 °C whenever immediate plasma preparation is not feasible to generate reliable data on blood-derived microRNA signatures.

Iohexol, platelet activation and thrombosis

1998 ◽  
Vol 39 (4) ◽  
pp. 349-354 ◽  
Author(s):  
K. S. Sakariassen ◽  
R. M. Barstad ◽  
M. J. A. G. Hamers ◽  
H. Stormorken
Keyword(s):  
Platelet Activation ◽  
Arterial Wall ◽  
Ex Vivo ◽  
Thrombus Formation ◽  
Human Model ◽  
Intravascular Thrombosis ◽  
Platelet Thrombus ◽  
In Vitro Platelet Activation ◽  
Platelet Secretion

Background: The nonionic monomer iohexol triggers in vitro platelet secretion of β-thromboglobulin (β-TG). This iohexol platelet activation may promote intravascular thrombosis. We studied this relationship by employing a human model of collagen-induced platelet thrombus formation at arterial flow. The ionic dimer ioxaglate, the nonionic dimer iodixanol, and glucose were included. Methods and Results: In vitro platelet activation as measured by β-TG secretion following a 1-min incubation of native blood with 50 vol% of iohexol was significant. Glucose solutions of 300, 580 and 825 mosmol, corresponding to the osmolalities of respectively iodixanol, ioxaglate and iohexol, increased the β-TG secretion in parallel with the osmolalities. Ioxaglate and iodixanol were virtually inert. Continuous infusion of iohexol or 580 or 825 mosmol glucose (40 vol%) into flowing native blood at an arterial wall shear rate of 2600 s−1 in an ex vivo collagen-induced platelet thrombus formation device triggered pronounced secretion of β-TG. However, the platelet thrombus formation in blood mixed with iohexol was within the same range as that observed with ioxaglate or iodixanol. Increasing glucose osmolality induced increasing β-TG secretion, which paralleled gradually decreasing platelet thrombus formation. Conclusion: Iohexol and 580 or 825 mosmol glucose trigger platelet secretion of β-TG. This secretion is not associated with enhanced collagen-induced platelet thrombus formation at high arterial shear.

Microparticle generation during in vitro platelet activation by anti-CD9 murine monoclonal antibodies

1991 ◽  
Vol 62 (5) ◽  
pp. 429-439 ◽  
Author(s):  
Shosaku Nomura ◽  
Hirokazu Nagata ◽  
Masahiko Suzuki ◽  
Koji Kondo ◽  
Shigetoshi Ohga ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Platelet Activation ◽  
Murine Monoclonal Antibodies ◽  
In Vitro Platelet Activation

Inhibitory effects of hydrogen on in vitro platelet activation and in vivo prevention of thrombosis formation

Life Sciences ◽  
2019 ◽  
Vol 233 ◽  
pp. 116700 ◽  
Author(s):  
Yun Wang ◽  
Ya-ping Wu ◽  
Ji-ju Han ◽  
Mao-qing Zhang ◽  
Chen-xi Yang ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Inhibitory Effects ◽  
Prevention Of Thrombosis ◽  
In Vitro Platelet Activation

Thrombospondin-1: a unique marker to identify in vitro platelet activation when monitoring in vivo processes

2010 ◽  
Vol 8 (8) ◽  
pp. 1809-1819 ◽  
Author(s):  
P. STARLINGER ◽  
H. P. MOLL ◽  
A. ASSINGER ◽  
C. NEMETH ◽  
K. HOETZENECKER ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Thrombospondin 1 ◽  
In Vitro Platelet Activation

In Vitro Platelet Activation by an Echo Contrast Agent

2003 ◽  
Vol 22 (4) ◽  
pp. 365-373 ◽  
Author(s):  
Kouichirou Shigeta ◽  
Nobuyuki Taniguchi ◽  
Kiyoka Omoto ◽  
Seiji Madoiwa ◽  
Yoichi Sakata ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Platelet Activation ◽  
In Vitro Platelet Activation
Sign in / Sign up

Export Citation Format

Share Document