scholarly journals Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome

2018 ◽  
Vol 17 (4) ◽  
pp. 481-486 ◽  
Author(s):  
Takuji Nishihara ◽  
Kazuya Matsumoto ◽  
Yoshihiko Hosoi ◽  
Yoshiharu Morimoto
2019 ◽  
Vol 493 ◽  
pp. S628
Author(s):  
V. Castañón Bernardo ◽  
C. Barneo Caragol ◽  
S. Rodríguez González ◽  
I. Vega Naredo ◽  
L. Sánchez Castro ◽  
...  

2018 ◽  
Vol 46 (11) ◽  
pp. 4624-4633 ◽  
Author(s):  
Xiang Lu ◽  
Zhengmu Wu ◽  
Min Wang ◽  
Weiwei Cheng

Objective This study was performed to investigate the effect of vitamin C (VitC) supplementation on the outcomes of in vitro fertilization–embryo transfer (IVF-ET) in patients with endometriosis (EMs). Methods A total of 280 patients with EMs underwent IVF-ET (VitC treatment group, n=160; VitC non-treatment group, n=120). An additional 150 patients who did not have EMs but underwent IVF-ET (control group) were also enrolled in this study. Superoxide dismutase (SOD), total antioxidant capacity (TAC), malondialdehyde (MDA) and reactive oxygen species (ROS) were measured to determine the role of VitC on oxidative stress markers in serum and follicular fluid (FF). Results In total, 245 patients with EMs and 132 patients without EMs underwent successful IVF-ET and follow-up. The serum or FF levels of VitC, SOD, and TAC were lower in the EMs than control group; however, the MDA and ROS levels in serum or FF were higher in the EMs than control group. After 2 months of VitC treatment, the serum VitC levels in serum and FF were significantly increased, while oxidative stress markers were unaffected. Conclusion Treatment with VitC oral formulation improved the serum and FF levels of VitC but did not affect oxidative stress markers in patients with EMs.


2020 ◽  
Vol 40 (6) ◽  
pp. 737-747
Author(s):  
Agnieszka Ścibior ◽  
Konrad A. Szychowski ◽  
Iwona Zwolak ◽  
Klaudia Dachowska ◽  
Jan Gmiński

2020 ◽  
Vol 14 (2) ◽  
pp. 24-33
Author(s):  
Raheleh Bagheri ◽  
Shiva Khezri ◽  
Gholamreza Najafi ◽  
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2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Katarzyna Olszak-Wąsik ◽  
Anna Bednarska-Czerwińska ◽  
Anita Olejek ◽  
Andrzej Tukiendorf

Background. Oxidative stress appears to be involved in oocyte growth and maturation that when impaired results in poor embryo quality and lower potential to implant. The biochemical microenvironment of the oocyte (follicular fluid (FF)) consists of hormones and other various substances regulating the balance between oxidants and antioxidants. Aim. The aim of this study was to examine the possible impact of selected biomarkers (“every day,” hormonal biomarkers, enzymatic and nonenzymatic antioxidants, and also oxidative stress markers) in serum and FF, on embryo quality and pregnancy success in infertile women undergoing infertility treatment. Methods. All 53 patients, mean age 34.7±4.1 years, with serum AMH level≥0.7 ng/mL, were diagnosed with idiopathic infertility. They were stimulated in short antagonist protocol, followed by in vitro fertilization (IVF-ICSI intracytoplasmatic sperm injection) and a single embryo transfer. Follicular fluid was aspirated from the first mature follicle. In statistical analyses the R software was used, then all data was assessed with the Shapiro-Wilk test, logistic regression, and later the receiver operating characteristic (ROC) curve was applied using “pROC” R package. Results. We did not observe any correlation between AMH and embryo quality and pregnancy rate. Statistically significant results were only found for biomarkers examined in follicular fluid. Greater levels of GPX in FF were associated with the increased chance of producing a high quality embryo (the optimal cut-off concentration was established at over 450 lU/L.) Regarding pregnancy success, increasing levels of GR (cut-off at 21 IU/L), CuZnSOD (cut-off at 9NU/mL), and GST (cut-off at 2.5 IU/L) resulted in lower chances of a successful pregnancy. Conclusion. Our results indicate that FF markers may have some advantages in predicting embryo quality and pregnancy over AMH. The GPX system seems to be mostly related to embryo quality and pregnancy.


2020 ◽  
Vol 37 (10) ◽  
pp. 1755-1764
Author(s):  
Luana Schmidt ◽  
Natália de Vargas Heck ◽  
Ingrid Ferreira ◽  
Gabriela Göethel ◽  
Sabrina Somacal ◽  
...  

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