High‐precision measurement of phenylalanine and glutamic acid δ 15 N by coupling ion‐exchange chromatography and purge‐and‐trap continuous‐flow isotope ratio mass spectrometry

2021 ◽  
Author(s):  
Lin Zhang ◽  
Wing‐man ( Charlotte) Lee ◽  
Ava Kreider‐Mueller ◽  
Evelyn Kuhnel ◽  
Jesus Baca ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Exchange ◽  
Glutamic Acid ◽  
Precision Measurement ◽  
Continuous Flow ◽  
Isotope Ratio ◽  
Isotope Ratio Mass Spectrometry ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
High Precision Measurement

Unexplained acidosis of malnutrition: a study by ion-exchange chromatography/mass spectrometry

2006 ◽  
Vol 20 (12) ◽  
pp. 1386-1389 ◽  
Author(s):  
P. J. Hilton ◽  
W. McKinnon ◽  
G. A. Lord ◽  
J.-M. R. Peron ◽  
L. G. Forni
Keyword(s):  
Mass Spectrometry ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatography Mass Spectrometry

Automated analysis for taurine in biological fluids and tissues.

1987 ◽  
Vol 33 (6) ◽  
pp. 835-837 ◽  
Author(s):  
H O Goodman ◽  
Z K Shihabi
Keyword(s):  
Ion Exchange ◽  
Continuous Flow ◽  
Biological Fluids ◽  
Automated Analysis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fluorescent Compound ◽  
Automated Method ◽  
Tissue Homogenates ◽  
Proteins And Peptides

Abstract We have developed an automated method of analysis for taurine, based on incorporating an ion-exchange chromatography column into the continuous-flow AutoAnalyzer (Technicon). After removal of proteins and peptides by dialysis, taurine is selectively eluted from an ion-exchange column and reacted with o-phthaldialdehyde to yield a fluorescent compound. The advantages of this method are: full automation with no need for sample deproteinization or cleanup; sensitivity, detecting as little as 5 mumol/L; speed (20 samples per hour); and flexibility. It can be used for assaying taurine in urine, plasma, cerebrospinal fluid, and tissue homogenates. This method can be adapted for assays of other metabolites.

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