Manual mass spectrometry de novo sequencing of the anionic host defense peptides of the Cuban Treefrog Osteopilus septentrionalis

2021 ◽  
Vol 35 (7) ◽  
Author(s):  
Tatiana Y. Samgina ◽  
Maria D. Tolpina ◽  
Alexey K. Surin ◽  
Sergey V. Kovalev ◽  
Roberto Alonso Bosch ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Defense ◽  
De Novo ◽  
De Novo Sequencing ◽  
Host Defense Peptides ◽  
Osteopilus Septentrionalis ◽  
Defense Peptides ◽  
Cuban Treefrog

scholarly journals Identification of new insect Host Defense Peptides (HDP) from Dung Beetles (Coleoptera: Scarabaeidae) transcriptomes

2020 ◽  
Author(s):  
Germán Alberto Téllez Ramirez ◽  
Juan Felipe Osorio-Méndez ◽  
Diana Carolina Henao Arias ◽  
Lily Johanna Toro S ◽  
Juliana Franco Castrillón ◽  
...  
Keyword(s):  
Host Defense ◽  
De Novo ◽  
Sequence Data ◽  
Structural Characteristics ◽  
Sequence Similarity ◽  
List Type ◽  
Sources Of Information ◽  
Host Defense Peptides ◽  
Transcriptomic Data ◽  
Defense Peptides

AbstractIntroductionThe Coleoptera Scarabaeidae family is one of the most diverse groups of insects on the planet, living in complex microbiological environments. Their immune systems have evolved with the generation of host defense peptides but only a small number of these peptides have been characterized.MethodsIn this work two sources of information were retrieved: 1) De novo transcriptomic data from two species of neotropical Scarabidae (Dichotomius satanas and Ontophagus curvicornis); 2) Sequence data deposited in available databases. A BLAST-based search against the transcriptomes with a subset of sequences representative of the Host Defense Peptides (HDP). The HDP were described with the cecropin, defensin, attacin, and coleoptericin families; their physical/chemical and structural properties were described.ResultsThis work reports 155 novel sequences of HDP identified in 9 transcriptomes from seven species from the Coleoptera order: D. satanas (n= 76; 49.03%), O. curvicornis (n= 23; 14.83%), T. dichotomus (n= 18; 11.61%), O. nigriventris (n= 10; 6.45%), Heterochelus sp (n= 6; 3.87%), O. conspicillatum (n= 18; 11.61%) and P. japonica (n= 4; 2.58%). These sequences were identified based on similarity to known HDP insect families. New members of defensins (n= 58; 37.42%), cecropins (n= 18; 11.61%), attancins (n= 41; 26.45%) and coleoptericins (n= 38; 24.52%), with their physicochemical, structural characteristics, and sequence relationship to other insect HDP were analyzed.Conclusions155 new HDP could be identified, based on similarity to known HDP insect families on nine transcriptome sequences of seven beetle species.HighlightsThis work identified 155 novel sequences of HDP found in nine transcriptomes from seven Coleoptera species.De novo transcriptomic data from two species of neotropical Scarabaeidae (Dichotomius satanas and Ontophagus curvicornis).In silico prediction of physicochemical properties, structural features, sequence similarity, and antimicrobial activity of Scarabaeidae HDP.

Geographically Distinct Expression Profile of Host Defense Peptides in the Skin of the Chinese Odorous Frog, Odorrana margaretae

2014 ◽  
Vol 4 (4) ◽  
pp. 288-297
Author(s):  
LING Guiying ◽  
LI Li ◽  
GAO Jiuxiang ◽  
YU Haining ◽  
WANG Yipeng ◽  
...  
Keyword(s):  
Host Defense ◽  
Expression Profile ◽  
Host Defense Peptides ◽  
Defense Peptides

Human Host Defense Peptides - Role in Maintaining Human Homeostasis and Pathological Processes

2017 ◽  
Vol 24 (7) ◽  
pp. 654-672 ◽  
Author(s):  
Malgorzata Anna Dawgul ◽  
Katarzyna E. Greber ◽  
Wieslaw Sawicki ◽  
Wojciech Kamysz
Keyword(s):  
Host Defense ◽  
Host Defense Peptides ◽  
Human Host ◽  
Defense Peptides

scholarly journals Mechanistic Fingerprinting Reveals Kinetic Signatures of Resistance to Daptomycin and Host Defense Peptides in Streptococcus mitis-oralis

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 404
Author(s):  
Michael R. Yeaman ◽  
Liana C. Chan ◽  
Nagendra N. Mishra ◽  
Arnold S. Bayer
Keyword(s):  
Flow Cytometry ◽  
Host Defense ◽  
Durable Resistance ◽  
Cross Resistance ◽  
Streptococcus Mitis ◽  
Host Defense Peptides ◽  
Strain Pair ◽  
Defense Peptides

Streptococcus mitis-oralis (S. mitis-oralis) infections are increasingly prevalent in specific populations, including neutropenic cancer and endocarditis patients. S. mitis-oralis strains have a propensity to evolve rapid, high-level and durable resistance to daptomycin (DAP-R) in vitro and in vivo, although the mechanism(s) involved remain incompletely defined. We examined mechanisms of DAP-R versus cross-resistance to cationic host defense peptides (HDPs), using an isogenic S. mitis-oralis strain-pair: (i) DAP-susceptible (DAP-S) parental 351-WT (DAP MIC = 0.5 µg/mL), and its (ii) DAP-R variant 351-D10 (DAP MIC > 256 µg/mL). DAP binding was quantified by flow cytometry, in-parallel with temporal (1–4 h) killing by either DAP or comparative prototypic cationic HDPs (hNP-1; LL-37). Multicolor flow cytometry was used to determine kinetic cell responses associated with resistance or susceptibility to these molecules. While overall DAP binding was similar between strains, a significant subpopulation of 351-D10 cells hyper-accumulated DAP (>2–4-fold vs. 351-WT). Further, both DAP and hNP-1 induced cell membrane (CM) hyper-polarization in 351-WT, corresponding to significantly greater temporal DAP-killing (vs. 351-D10). No strain-specific differences in CM permeabilization, lipid turnover or regulated cell death were observed post-exposure to DAP, hNP-1 or LL-37. Thus, the adaptive energetics of the CM appear coupled to the outcomes of interactions of S. mitis-oralis with DAP and selected HDPs. In contrast, altered CM permeabilization, proposed as a major mechanism of action of both DAP and HDPs, did not differentiate DAP-S vs. DAP-R phenotypes in this S. mitis-oralis strain-pair.

ON PREPROCESSING AND ANTISYMMETRY IN DE NOVO PEPTIDE SEQUENCING: IMPROVING EFFICIENCY AND ACCURACY

2008 ◽  
Vol 06 (03) ◽  
pp. 467-492 ◽  
Author(s):  
KANG NING ◽  
NAN YE ◽  
HON WAI LEONG
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Computational Models ◽  
De Novo ◽  
Noise Removal ◽  
Peptide Sequencing ◽  
De Novo Sequencing ◽  
Tandem Mass ◽  
De Novo Peptide Sequencing ◽  
De Novo Peptide

Peptide sequencing plays a fundamental role in proteomics. Tandem mass spectrometry, being sensitive and efficient, is one of the most commonly used techniques in peptide sequencing. Many computational models and algorithms have been developed for peptide sequencing using tandem mass spectrometry. In this paper, we investigate general issues in de novo sequencing, and present results that can be used to improve current de novo sequencing algorithms. We propose a general preprocessing scheme that performs binning, pseudo-peak introduction, and noise removal, and present theoretical and experimental analyses on each of the components. Then, we study the antisymmetry problem and current assumptions related to it, and propose a more realistic way to handle the antisymmetry problem based on analysis of some datasets. We integrate our findings on preprocessing and the antisymmetry problem with some current models for peptide sequencing. Experimental results show that our findings help to improve accuracies for de novo sequencing.

scholarly journals Nano-mechanical in-process monitoring of antimicrobial poration in model phospholipid bilayers

RSC Advances ◽  
2017 ◽  
Vol 7 (31) ◽  
pp. 19081-19084
Author(s):  
Andrea Valsesia ◽  
Patrizia Iavicoli ◽  
Helen Lewis ◽  
Cloé Desmet ◽  
Dora Mehn ◽  
...  
Keyword(s):  
Process Monitoring ◽  
Host Defense ◽  
Phospholipid Bilayers ◽  
Host Defense Peptides ◽  
Defense Peptides ◽  
Membrane Poration

Nanomechanical monitoring of known mechanisms of membrane poration mediated by host defense peptides is reported.

A comparison of host-defense peptides in skin secretions of female Xenopus laevis×Xenopus borealis and X. borealis×X. laevis F1 hybrids

Peptides ◽  
2013 ◽  
Vol 45 ◽  
pp. 1-8 ◽  
Author(s):  
Milena Mechkarska ◽  
Manju Prajeep ◽  
Jérôme Leprince ◽  
Hubert Vaudry ◽  
Mohammed A. Meetani ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Host Defense ◽  
F1 Hybrids ◽  
Host Defense Peptides ◽  
Skin Secretions ◽  
Defense Peptides ◽  
Xenopus Borealis

scholarly journals Host-defense peptides

1996 ◽  
Vol 14 (7) ◽  
pp. 804-804
Author(s):  
Robert L. Erwin
Keyword(s):  
Host Defense ◽  
Host Defense Peptides ◽  
Defense Peptides

Host Defense Peptides as immunomodulators: the other side of the coin

Peptides ◽  
2021 ◽  
pp. 170644
Author(s):  
Ernesto M. Martell ◽  
Melaine González ◽  
Ludger Ständker ◽  
Anselmo J. Otero-González
Keyword(s):  
Host Defense ◽  
The Other ◽  
Host Defense Peptides ◽  
Defense Peptides
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