Pharmacokinetics and tissue distribution study of 18 bioactive components in healthy and chronic heart failure rats after oral administration of Qi‐Shen‐Ke‐Li formula using ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry

2021 ◽  
Vol 35 (8) ◽  
Author(s):  
Hui Zhou ◽  
Yang He ◽  
Zhong Zheng ◽  
Junpeng Xing ◽  
Zhiqiang Liu ◽  
...  
Keyword(s):  
Heart Failure ◽  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Triple Quadrupole ◽  
Bioactive Components ◽  
Quadrupole Mass ◽  
Distribution Study ◽  
Tissue Distribution Study

scholarly journals Simultaneous quantification of bioactive components in Chinese herbal spirits by ultra-high performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC–QQQ–MS/MS)

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Yan Hu ◽  
Zhe Wang ◽  
Fangbo Xia ◽  
Wen Yang ◽  
Yuan-Cai Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Structural Diversity ◽  
Triple Quadrupole ◽  
Bioactive Components ◽  
Quadrupole Mass ◽  
Quadrupole Mass Spectrometry ◽  
Chinese Herbal

Abstract Background The Chinese medicinal wine made from herbal medicines became prevalent among Chinese people. The Chinese herbal spirit is composed of several herbal extracts, and has the certain health functions, such as anti-fatigue and immune regulation. The quality evaluation of Chinese herbal spirit is greatly challenged by the enormous and complex components with great structural diversity and wide range of concentration distribution. Methods An ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS) with multiple reaction monitoring (MRM) method was developed to simultaneously determine forty-three bioactive components in the Chinese herbal spirits produced by year 2014 and 2018. Results Quantitative results showed that 11 components, i.e.., puerarin (5), purpureaside C (7), daidzin (8), echinacoside (9), acteoside (15), epimedin B (22), epimedin C (23), icariin (24), eugenol (27), chikusetsusaponin iva (30) and Z-ligustilide (40), significantly decreased along with the increasing years of storage, while 5 compounds, i.e.., geniposidic acid (1), protocatechuic acid (2), crustecdysone (14), daidzein (18) and icariside I (35), were basically stable in all samples across the years. Concusion The established method allowing to simultaneously determined 43 components with wide structural diversity and trace amounts will facilitate the quality control research of Chinese herbal spirits.

Sign in / Sign up

Export Citation Format

Share Document