Flash desorption of low‐volatility compounds deposited on a heated solid substrate (90°C) by dripping liquid methanol

Objective: Amylases enzymes hydrolyze starch molecules to produce diverse products including dextrins, and progressively smaller polymers. These include glucose units linked through α-1- 1, α-1-4, α-1-6, glycosidic bonds. Methods: This enzyme carrying an (α /β) 8 or TIM barrel structure is also produced containing the catalytic site residues. These groups of enzymes possess four conserved regions in their primary sequence. In the Carbohydrate-Degrading Enzyme (CAZy) database, α-amylases are classified into different Glycoside Hydrolase Families (GHF) based on their amino acid sequence. The present objective was to study one such enzyme based on its molecular characterization after purification in our laboratory. Its main property of solid-natural starch degradation was extensively investigated for its pharmaceutical/ industrial applications. Results: Amylase producing bacteria Bacillus cereus sm-sr14 (Accession no. KM251578.1) was purified to homogeneity on a Seralose 6B-150 gel-matrix and gave a single peak during HPLC. MALDITOF mass-spectrometry with bioinformatics studies revealed its significant similarity to α/β hydrolase family. The enzyme showed an efficient application; favourable Km, Vmax and Kcat during the catalysis of different natural solid starch materials. Analysis for hydrolytic product showed that this enzyme can be classified as the exo-amylase asit produced a significant amount of glucose. Conclusion: Besides the purified enzyme, the present organism Bacillus cereus sm-sr14 could degrade natural solid starch materials like potato and rice up to the application level in the pharmaceutical/ industrial field for alcohol production.

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Agro-Industrial Residues as Solid Substrate for α-Amylase Production Using Solid State Fermentation by Filamentous Fungi: A Review

Bioscience Biotechnology Research Communications ◽

10.21786/bbrc/13.2/28 ◽

2020 ◽

Vol 13 (2) ◽

pp. 550-555

Author(s):

Santosh Kumar Mishra

Keyword(s):

Solid State ◽

Filamentous Fungi ◽

Solid State Fermentation ◽

Solid Substrate ◽

Industrial Residues ◽

Amylase Production

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Sarocladium and Lecanicillium Associated with Maize Seeds and Their Potential to Form Selected Secondary Metabolites

Biomolecules ◽

10.3390/biom11010098 ◽

2021 ◽

Vol 11 (1) ◽

pp. 98

Author(s):

Lidia Błaszczyk ◽

Agnieszka Waśkiewicz ◽

Karolina Gromadzka ◽

Katarzyna Mikołajczak ◽

Jerzy Chełkowski

Keyword(s):

Large Subunit ◽

Solid Substrate ◽

Rrna Gene ◽

Decenoic Acid ◽

Bioactive Substances ◽

Lecanicillium Lecanii ◽

Maize Seeds ◽

Internally Transcribed Spacer ◽

First Time

The occurrence and diversity of Lecanicillium and Sarocladium in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate Sarocladium and Lecanicillium communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 Sarocladium zeae strains, three Sarocladium strictum and five Lecanicillium lecanii isolates. The assay on solid substrate showed that S. zeae and S. strictum can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of S. strictum and L. lecanii. The production of toxic, insecticidal and antibacterial compounds in cultures of S. strictum, S. zeae and L. lecanii suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.

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Optical nanomanipulation on solid substrates via optothermally-gated photon nudging

Nature Communications ◽

10.1038/s41467-019-13676-3 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 7

Author(s):

Jingang Li ◽

Yaoran Liu ◽

Linhan Lin ◽

Mingsong Wang ◽

Taizhi Jiang ◽

...

Keyword(s):

Colloidal Particles ◽

Solid Substrate ◽

Optical Scattering ◽

Functional Nanostructures ◽

Non Invasive ◽

Solid Substrates ◽

All Optical ◽

Scattering Force ◽

Reconfigurable Assembly

AbstractConstructing colloidal particles into functional nanostructures, materials, and devices is a promising yet challenging direction. Many optical techniques have been developed to trap, manipulate, assemble, and print colloidal particles from aqueous solutions into desired configurations on solid substrates. However, these techniques operated in liquid environments generally suffer from pattern collapses, Brownian motion, and challenges that come with reconfigurable assembly. Here, we develop an all-optical technique, termed optothermally-gated photon nudging (OPN), for the versatile manipulation and dynamic patterning of a variety of colloidal particles on a solid substrate at nanoscale accuracy. OPN takes advantage of a thin surfactant layer to optothermally modulate the particle-substrate interaction, which enables the manipulation of colloidal particles on solid substrates with optical scattering force. Along with in situ optical spectroscopy, our non-invasive and contactless nanomanipulation technique will find various applications in nanofabrication, nanophotonics, nanoelectronics, and colloidal sciences.

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Metastability in Monolayer Films Transferred onto Solid Substrates by the Langmuir-Blodgett Method: IR Evidence for Transfer-Induced Phase Transitions

Applied Spectroscopy ◽

10.1366/0003702944027309 ◽

1994 ◽

Vol 48 (10) ◽

pp. 1196-1203 ◽

Cited By ~ 18

Author(s):

Fazale R. Rana ◽

Suci Widayati ◽

Brian W. Gregory ◽

Richard A. Dluhy

Keyword(s):

Fatty Acid ◽

Conformational Changes ◽

Structural Changes ◽

High Surface ◽

Solid Substrate ◽

Water Interface ◽

Monolayer Films ◽

Langmuir Blodgett ◽

Monomolecular Film ◽

Air Water Interface

The rate at which a monomolecular film is deposited onto a solid substrate in the Langmuir-Blodgett process of preparing supported monolayer films influences the final structure of the transferred film. Attenuated total reflectance infrared spectroscopic studies of monolayers transferred to germanium substrates show that the speed at which the substrate is drawn through the air/water interface influences the final conformation in the hydrocarbon chains of amphiphilic film molecules. This transfer-induced effect is especially evident when the monolayer is transferred from the expanded region of surface-pressure-molecular-area isotherms at low surface pressures; the effect is minimized when the film molecules are transferred from condensed phases at high surface pressures. This phenomenon has been observed for both a fatty acid and a phospholipid, which suggests that these conformational changes may occur in a variety of hydrocarbon amphiphiles transferred from the air/water interface. This conformational ordering may be due to a kinetically limited phase transition taking place in the meniscus formed between the solid substrate and aqueous subphase. In addition, the results obtained for both the phospholipid and fatty acid suggest that the structure of the amphiphile may help determine the extent and nature of the transfer-speed-induced structural changes taking place in the monomolecular film.

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