scholarly journals Coated glass capillaries as SPME devices for DART mass spectrometry

2020 ◽  
Vol 34 (23) ◽  
Author(s):  
Robert Cody ◽  
Simin D. Maleknia
Keyword(s):  
Mass Spectrometry ◽  
Coated Glass ◽  
Dart Mass Spectrometry ◽  
Glass Capillaries

Thrombin Stimulated Platelet Accumulation on Protein Coated Glass Capillaries: Role of Adhesive Platelet α-Granule Proteins

1989 ◽  
Vol 62 (03) ◽  
pp. 989-995 ◽  
Author(s):  
Juliette N Mulvihill ◽  
J Andrew Davies ◽  
Florence Toti ◽  
Jean-Marie Freyssinet ◽  
Jean-Pierre Cazenave
Keyword(s):  
Human Platelets ◽  
Capillary Perfusion ◽  
Coated Glass ◽  
Bovine Collagen ◽  
Glass Capillaries ◽  
Human Thrombin ◽  
Platelet Accumulation ◽  
Granule Proteins ◽  
Von Willebrand

SummaryThe generation of trace amounts of thrombin at artificial surfaces in contact with blood is likely to be a contributing factor in thrombosis on biomaterials. Using an in vitro capillary perfusion system, platelet accumulation on glass surfaces, uncoated or precoated with purified bovine collagen or human plasma proteins, was determined in the presence or absence of preadsorbed purified human thrombin. Static adsorption for 15 min at 22° C from solutions of thrombin 100 NIH units (33 μg)/ml gave surface concentrations in the range 0.019-0.101 μg/cm2. Protein coated capillaries, thrombin treated or untreated, were perfused for 2 min at 37° C with suspensions of washed 111In-labeled human platelets in Tyrode's-albumin buffer containing 40% washed red blood cells, under conditions of controlled, non pulsatile laminar flow (50 s−1 or 2,000 s−1). Platelet accumulation was increased in the presence of surface adsorbed thrombin on uncoated and albumin or fibrinogen coated glass but little affected on fibronectin or collagen coated glass. On von Willebrand factor (vWF) coated glass, thrombin enhancement was observed only at high shear forces. In experiments using antibodies against human platelet α-granule proteins, thrombin stimulated platelet deposition in uncoated glass capillaries was inhibited at 2,000 s−1 by anti-vWF and to a lesser extent by anti-fibrinogen but not by antithrombospondin antibodies.

Rapid Detection of Vegetable Fat Additives in Condensed Milk by DART Mass Spectrometry

2021 ◽  
Vol 76 (13) ◽  
pp. 1493-1498
Author(s):  
S. V. Goryainov ◽  
C. Esparza ◽  
A. R. Borisova ◽  
K. Fadi ◽  
E. O. Rystsova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Rapid Detection ◽  
Condensed Milk ◽  
Dart Mass Spectrometry ◽  
Vegetable Fat
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