A top down approach to protein structural studies using chemical cross-linking and Fourier transform mass spectrometry

2003 ◽  
Vol 17 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Gary H. Kruppa ◽  
Joseph Schoeniger ◽  
Malin M. Young
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Fourier Transform Mass Spectrometry ◽  
Cross Linking ◽  
Structural Studies ◽  
Top Down ◽  
Chemical Cross Linking

Monitoring Conformational Changes in Protein Complexes Using Chemical Cross-Linking and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: The Effect of Calcium Binding on the Calmodulin—Melittin Complex

2007 ◽  
Vol 13 (4) ◽  
pp. 281-290 ◽  
Author(s):  
Petr Novak ◽  
Vladimir Havlicek ◽  
Peter J. Derrick ◽  
Kyle A. Beran ◽  
Sajid Bashir ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Conformational Changes ◽  
Calcium Binding ◽  
Cyclotron Resonance ◽  
Structural Changes ◽  
Cross Linking ◽  
Ion Cyclotron Resonance ◽  
Ion Cyclotron ◽  
Chemical Cross Linking

Calmodulin is an EF hand calcium binding protein. Its binding affinities to various protein/peptide targets often depend on the conformational changes induced by the binding of calcium. One such target is melittin, which binds tightly to calmodulin in the presence of calcium, and inhibits its function. Chemical cross-linking combined with Fourier transform ion cyclotron resonance mass spectrometry has been employed to investigate the coordination of calmodulin and melittin in the complex at different concentrations of calcium. This methodology can be used to monitor structural changes in proteins induced by ligand binding and to study the effects these changes have on non-covalent interactions between proteins. Cross-linking results indicate that the binding place of the first melittin in the calcium-free calmodulin form is the same as in the calcium-loaded calmodulin/melittin complex.

scholarly journals Micro-heterogeneity of human saliva peptide P-C characterized by high-resolution top-down fourier-transform mass spectrometry

2010 ◽  
Vol 21 (5) ◽  
pp. 868-877 ◽  
Author(s):  
Frédéric Halgand ◽  
Vlad Zabrouskov ◽  
Sara Bassilian ◽  
Puneet Souda ◽  
David T. Wong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
High Resolution ◽  
Fourier Transform Mass Spectrometry ◽  
Human Saliva ◽  
Top Down

Top-Down Approaches for Measuring Expression Ratios of Intact Yeast Proteins Using Fourier Transform Mass Spectrometry

2006 ◽  
Vol 78 (3) ◽  
pp. 686-694 ◽  
Author(s):  
Yi Du ◽  
Bryan A. Parks ◽  
Seyoung Sohn ◽  
Kurt E. Kwast ◽  
Neil L. Kelleher
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Fourier Transform Mass Spectrometry ◽  
Top Down
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