LysargiNase enhances protein identification on the basis of trypsin on formalin‐fixed paraffin‐embedded samples

2019 ◽  
Vol 33 (17) ◽  
pp. 1381-1389 ◽  
Author(s):  
Shu Liu ◽  
Feng Xu ◽  
Yanan Yin ◽  
Junling Zhang ◽  
Fuqiang Wang ◽  
...  
Keyword(s):  
Protein Identification ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals State-of-the-Art of Profiling Immune Contexture in the Era of Multiplexed Staining and Digital Analysis to Study Paraffin Tumor Tissues

Cancers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 247 ◽  
Author(s):  
Edwin Parra ◽  
Alejandro Francisco-Cruz ◽  
Ignacio Wistuba
Keyword(s):  
Cancer Prevention ◽  
Protein Identification ◽  
Tissue Microarrays ◽  
Formalin Fixed Paraffin ◽  
Tumor Tissues ◽  
Formalin Fixed Paraffin Embedded ◽  
Immune Contexture ◽  
New Treatment ◽  
Cancer Tissues ◽  
Formalin Fixed

Multiplexed platforms for multiple epitope detection have emerged in the last years as very powerful tools to study tumor tissues. These revolutionary technologies provide important visual techniques for tumor examination in formalin-fixed paraffin-embedded specimens to improve the understanding of the tumor microenvironment, promote new treatment discoveries, aid in cancer prevention, as well as allowing translational studies to be carried out. The aim of this review is to highlight the more recent methodologies that use multiplexed staining to study simultaneous protein identification in formalin-fixed paraffin-embedded tumor tissues for immune profiling, clinical research, and potential translational analysis. New multiplexed methodologies, which permit the identification of several proteins at the same time in one single tissue section, have been developed in recent years with the ability to study different cell populations, cells by cells, and their spatial distribution in different tumor specimens including whole sections, core needle biopsies, and tissue microarrays. Multiplexed technologies associated with image analysis software can be performed with a high-quality throughput assay to study cancer specimens and are important tools for new discoveries. The different multiplexed technologies described in this review have shown their utility in the study of cancer tissues and their advantages for translational research studies and application in cancer prevention and treatments.

scholarly journals Improved protein extraction and protein identification from archival formalin-fixed paraffin-embedded human aortas

2013 ◽  
Vol 7 (3-4) ◽  
pp. 217-224 ◽  
Author(s):  
Zongming Fu ◽  
Kun Yan ◽  
Avraham Rosenberg ◽  
Zhicheng Jin ◽  
Barbara Crain ◽  
...  
Keyword(s):  
Protein Identification ◽  
Protein Extraction ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
Sign in / Sign up

Export Citation Format

Share Document