Surface modification of chitosan films with a fibronectin fragment-DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

2019 ◽  
Vol 33 (4) ◽  
pp. 336-342 ◽  
Author(s):  
Martina Saccani ◽  
Ludovica Parisi ◽  
Carlo Bergonzi ◽  
Annalisa Bianchera ◽  
Carlo Galli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Surface Modification ◽  
Cell Activity ◽  
Dna Aptamer ◽  
Osteoblastic Cell ◽  
Fibronectin Fragment ◽  
Chitosan Films

Decreased potassium stimulates bone resorption

1997 ◽  
Vol 272 (6) ◽  
pp. F774-F780 ◽  
Author(s):  
D. A. Bushinsky ◽  
D. R. Riordon ◽  
J. S. Chan ◽  
N. S. Krieger
Keyword(s):  
Bone Resorption ◽  
Collagen Synthesis ◽  
Cell Activity ◽  
Bone Cell ◽  
Osteoblastic Cell ◽  
Enzyme Release ◽  
Osteoblastic Cell Line ◽  
Glucuronidase Activity ◽  
K Concentration ◽  
Low K

Metabolic acidosis induces net calcium efflux (JCa+) from cultured bone, in part, through an increase in osteoclastic resorption and a decrease in osteoblastic formation. In humans provision of base as potassium (K+) citrate, but not sodium (Na+) citrate, reduces urine Ca (UCa), and oral KHCO3 decreases bone resorption and UCa in postmenopausal women. Potassium deprivation alone leads to an increase in UCa. To determine whether decreased extracellular K+ concentration ([K+]) at a constant pH, PCO2, and [HCO-3] alters JCa+ and bone cell activity, we measured JCa+, osteoblastic collagen synthesis, and osteoclastic beta-glucuronidase release from neonatal mouse calvariae cultured for 48 h in medium of varying [K+]. Calvariae were cultured in control medium (approximately 4 mM [K+]) or medium with mildly low K+ (MLK, approximately 3 mM [K+]), very low K+ (VLK, approximately 2 mM [K+]), or extremely low K+ (ELK, approximately 1 mM [K+]) (n > or = 9 in each group). Compared with control, ELK, but not MLK or VLK, resulted in a marked increase in JCa+ and an increase in beta-glucuronidase release and a decrease in collagen synthesis. JCa+ was correlated directly with medium beta-glucuronidase activity and inversely with collagen synthesis. To determine whether the reduction in medium [K+] was associated with a decrease in intracellular pH (pHi), we measured pHi in MC3T3-E1 cells, a mouse osteoblastic cell line. Incubation in 1 mM [K+] led to a significant decrease in pHi compared with 3 mM [K+]. Thus incubation in a reduced [K+] medium stimulates JCa+ and osteoclastic enzyme release and inhibits osteoblastic collagen synthesis, which may be mediated by a reduction in bone cell pH.

Pamidronic acid-grafted nHA/PLGA hybrid nanofiber scaffolds suppress osteoclastic cell viability and enhance osteoblastic cell activity

2016 ◽  
Vol 4 (47) ◽  
pp. 7596-7604 ◽  
Author(s):  
Adnan Haider ◽  
Davy-louis Versace ◽  
Kailash Chandra Gupta ◽  
Inn-Kyu Kang
Keyword(s):  
Bone Resorption ◽  
Cell Viability ◽  
Pamidronic Acid ◽  
Cell Activity ◽  
Osteoblastic Cell ◽  
Osteoclast Activity ◽  
Nanofiber Scaffolds ◽  
Hybrid Nanofiber ◽  
Osteoclastic Cell

Controlling osteoclast activity helps in prevention of bone resorption.

scholarly journals Surface Modification of Film Chitosan Materials With Aldehydes for Wettability and Biodegradation Control

2021 ◽  
Author(s):  
Ekaterina Bryuzgina ◽  
Vitaliya Yartseva ◽  
Evgeny Bryuzgin ◽  
Oleg Tuzhikov ◽  
Alexander Navrotskiy
Keyword(s):  
Surface Modification ◽  
Wound Dressings ◽  
Molecular Characteristics ◽  
Modified Chitosan ◽  
Skin Cell ◽  
Hydrophilic Lipophilic Balance ◽  
Chitosan Films ◽  
The Creation ◽  
External Wound ◽  
Cytotoxic Index

Abstract Chitosan is one of prospective polymer for use in regenerative medicine which has unique properties such as biocompatibility, biodegradability, antimicrobial, anti-inflammatory, and antitumor potency. In this article, we study the peculiarities of the surface modification of chitosan films with carbonyl-containing compounds, which differed both in molecular characteristics and in their hydrophilic and hydrophobic properties. The potential for controlling the biodegradation of the resulting materials has been established, which can be used in the creation of wound dressings. Both the destruction time and lyophilic properties of the surface depend on the length of the modifier's hydrocarbon radical. The contact angle and water absorption of obtained film materials correlate with hydrophobicity, which estimated by the calculated value of the hydrophilic–lipophilic balance (HLB). The cytotoxicity of modified chitosan films was studied, and it was found that they are non-toxic (cytotoxic index of <50%) for human skin cell cultures, which shows their potential for use in the creation of materials for skin protection and external wound healing.

Surface Functionalization of Silicon MEMS Biochemical Sensors for the Detection of Foodborne Pathogens

2021 ◽  
Author(s):  
Md Ebrahim Khalil Bhuiyan ◽  
Dustin Smith ◽  
Eric J. Voss ◽  
Chin-Chuan Wei ◽  
Mohammad Shavezipur
Keyword(s):  
Surface Modification ◽  
Surface Functionalization ◽  
Foodborne Pathogens ◽  
Silicon Surface ◽  
Chemical Species ◽  
Biological Species ◽  
Dna Aptamer ◽  
Native Oxide ◽  
Sensor Surface ◽  
E Coli

Abstract This work presents the surface modification of silicon chips as a platform for silicon-based biosensors with applications aiming for the detection of foodborne bacteria in aqueous solution. The detection requires high selectivity as the solution may contain a variety of biological species, which affect the outcome of the sensing process. The silicon surface is functionalized by a self-assembled monolayer (SAM) with thiol groups followed by immobilizing a thiol-linked DNA aptamer. The DNA aptamer used in this work has reported to recognize a biological species, E. coli ATCC 25922. The presence of DNA aptamer on the sensor surface allows the capture of the specific E. coli cells on the surface, while other potential biological (and chemical) species would not attach to the sensor surface, thus improving the selectivity of the sensor. The uniform formation of the SAM on the surface is an important step toward uniformly coating the sensor surface with the desired DNA aptamer. The SAM is created on the silicon surface by surface modification with the MPTS (3-mercaptopropyl trimethoxy silane) solution. Then the aptamer DNA solution is applied as droplets on the chip followed by a cure process. The attachment of the SAM and DNA aptamers are verified by atomic force microscopy (AFM). The surface functionalization presented in this work can be used for sensors made of silicon coated with a thin layer of native oxide, and can be adopted for detection of other cells and biological agents using the proper SAM and DNA aptamer.

Mass Spectrometry Guided Surface Modification of the Tellurate Ion Templated 36-Nucleus Silver Alkynl Nanocluster

CrystEngComm ◽  
2021 ◽  
Author(s):  
Lin-Lin Hou ◽  
Hong-Jing Li ◽  
Dong-Nan Yu ◽  
Xun Cheng ◽  
Zi-Xuan Yao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Surface Modification ◽  
Esi Ms

Under the guidance of the ESI-MS result of our former reported cluster [TeO6@Ag36(C≡CtBu)18(tfa)12] (1, tfa = trifluoroacetate), we fabricate a new 36-nucleus silver-alkynyl cluster substituted by four pentafluorobenzoates, named [TeO6@Ag36(C≡CtBu)18(tfa)8(F5PhCO2)4]...

1012 Influences of Microbubble-Induced Hyperoxia on Osteoblastic Cell Activity Cultured in 3-D Collagen Gel

2007 ◽  
Vol 2007.5 (0) ◽  
pp. 225-226
Author(s):  
Kosaku KURATA ◽  
Hiroyasu TANIGUCHI ◽  
Takanobu FUKUNAGA ◽  
Junpei MATSUDA ◽  
Hidehiko HIGAKI
Keyword(s):  
Cell Activity ◽  
Collagen Gel ◽  
Osteoblastic Cell

scholarly journals Electromagnetic Wave Irradiation Promotes Osteoblastic Cell Proliferation and Up-Regulates Growth Factors via Activation of the ERK1/2 and p38 MAPK Pathways

2015 ◽  
Vol 35 (2) ◽  
pp. 601-615 ◽  
Author(s):  
Hiromichi Yumoto ◽  
Kouji Hirao ◽  
Toshihiko Tominaga ◽  
Naoki Bando ◽  
Kanako Takahashi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Electromagnetic Wave ◽  
Growth Factors ◽  
Growth Factor ◽  
P38 Mapk ◽  
Cell Activity ◽  
Specific Inhibitor ◽  
Osteoblastic Cell ◽  
Periodontal Tissues ◽  
Mapk Pathways

Background/Aims: Periodontitis with bone resorption is caused by inflammatory reactions to bacterial infection. We recently reported that electromagnetic wave irradiation (EMWI) has bactericidal effects. However, the effects of EMWI on periodontal tissues remain unclear. This study was aimed to investigate the effects of EMWI on osteoblasts. Methods: Osteoblastic cells MC3T3-E1 were treated with EMWI (500-1,000 kHz, 5 times, 1 sec/time). Cell growth and cytotoxicity were determined by cell proliferation assays and measurement of lactate dehydrogenase release, respectively. Gene expression and protein production of growth factors were analyzed using real-time PCR and ELISA, respectively. EMWI-activated cellular signal transduction pathways were investigated by immunoblotting and blocking assay with specific inhibitors. Results: Osteoblasts proliferation was significantly enhanced 3 days after EMWI and no cytotoxicity was observed. EMWI up-regulated various growth factors, such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). EMWI induced ERK1/2, p38 MAPK and SAPK/JNK phosphorylation within 5 min, and the production of PDGF-ΑΑ and VEGF was partially reduced by MAPK-specific inhibitor. Conclusion: These findings demonstrated that EMWI increases osteoblastic cell activity and the expression of growth factors via ERK1/2 and p38 MAPK pathways and suggested that EMWI may be beneficial to bone tissue repair such as periodontitis.

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