Automated high-capacity on-line extraction and bioanalysis of dried blood spot samples using liquid chromatography/high-resolution accurate mass spectrometry

2014 ◽  
Vol 28 (22) ◽  
pp. 2415-2426 ◽  
Author(s):  
Regina V. Oliveira ◽  
Jack Henion ◽  
Enaksha R. Wickremsinhe
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Capacity ◽  
Dried Blood Spot ◽  
Line Extraction ◽  
On Line ◽  
Accurate Mass Spectrometry ◽  
Blood Spot ◽  
High Resolution Accurate Mass

scholarly journals Metabolome Variation between Strains of Microcystis aeruginosa by Untargeted Mass Spectrometry

Toxins ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 723 ◽  
Author(s):  
Marianne Racine ◽  
Ammar Saleem ◽  
Frances R. Pick
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Microcystis Aeruginosa ◽  
Ultra Performance Liquid Chromatography ◽  
Widespread Species ◽  
Accurate Mass Spectrometry ◽  
The Common ◽  
Different Strains ◽  
High Resolution Accurate Mass

Cyanobacteria are notorious for their potential to produce hepatotoxic microcystins (MCs), but other bioactive compounds synthesized in the cells could be as toxic, and thus present interest for characterization. Ultra performance liquid chromatography and high-resolution accurate mass spectrometry (UPLC-QTOF-MS/MS) combined with untargeted analysis was used to compare the metabolomes of five different strains of the common bloom-forming cyanobacterium, Microcystis aeruginosa. Even in microcystin-producing strains, other classes of oligopeptides including cyanopeptolins, aeruginosins, and aerucyclamides, were often the more dominant compounds. The distinct and large variation between strains of the same widespread species highlights the need to characterize the metabolome of a larger number of cyanobacteria, especially as several metabolites other than microcystins can affect ecological and human health.

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