Investigation of noncovalent interactions of aconitine with duplex, triplex and G-quadruplex DNA by electrospray ionization mass spectrometry

2014 ◽  
Vol 28 (7) ◽  
pp. 839-842 ◽  
Author(s):  
Lei Ma ◽  
Shu Liu ◽  
Fengrui Song ◽  
Zhiqiang Liu ◽  
Shuying Liu
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Noncovalent Interactions ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Quadruplex Dna ◽  
G Quadruplex

Developments in Electrospray Ionization Mass Spectrometry of Non-Covalent DNA–Ligand Complexes

2011 ◽  
Vol 64 (6) ◽  
pp. 705 ◽  
Author(s):  
Jennifer L. Beck
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Short Review ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Quadruplex Dna ◽  
Esi Ms ◽  
Selective Ligands ◽  
Ligand Interactions

Many anti-cancer drugs function by binding non-covalently to double-stranded (ds) DNA. Electrospray ionization mass spectrometry (ESI-MS) has emerged over the past decade as a sensitive technique for the determination of stoichiometries and relative binding affinities of DNA–ligand interactions. The chromosome contains nucleotide sequences, for example, guanosine-rich regions, that predispose them to the formation of higher order structures such as quadruplex DNA (qDNA). Sequences that form qDNA are found in the telomeres. The proposal that ligands that stabilize qDNA might interfere with the activity of telomerase in cancer cells has stimulated the search for ligands that are selective for qDNA over dsDNA. The insights gained from the development of ESI-MS methods for analysis of non-covalent dsDNA–ligand complexes are now being applied in the search for qDNA-selective ligands. ESI-MS is a useful first-pass screening technique for qDNA-binding ligands. This short review describes some experimental considerations for ESI-MS analysis of DNA–ligand complexes, briefly addresses the question of whether non-covalent DNA–ligand complexes are faithfully transferred from solution to the gas phase, discusses ion mobility mass spectrometry as a technique for probing this issue, and highlights some recent ESI-MS studies of qDNA-selective ligands.

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