scholarly journals Effects of euthanasia method on stable-carbon and stable-nitrogen isotope analysis for an ectothermic vertebrate

2013 ◽  
Vol 27 (15) ◽  
pp. 1814-1814
Author(s):  
Meredith A. Atwood
Keyword(s):  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Stable Carbon ◽  
Stable Nitrogen Isotope ◽  
Ectothermic Vertebrate ◽  
Nitrogen Isotope Analysis

Preservation of blood and tissue samples for stable-carbon and stable-nitrogen isotope analysis

1997 ◽  
Vol 75 (10) ◽  
pp. 1720-1723 ◽  
Author(s):  
Keith A. Hobson ◽  
Mark L. Gloutney ◽  
H. Lisle Gibbs
Keyword(s):  
Glass Fibre ◽  
Filter Paper ◽  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Ovis Aries ◽  
Stable Carbon ◽  
Stable Nitrogen Isotope ◽  
Freeze Dried ◽  
Lysis Buffer ◽  
Nitrogen Isotope Analysis

Researchers engaged in collecting animal material for stable-carbon and stable-nitrogen isotope analysis are frequently faced with the need to preserve tissues prior to transportation to the laboratory. In many cases, freezing is not possible in the field, so we investigated the potential of several techniques for preserving tissues for this purpose. We also included preservation techniques used for DNA analyses in order to evaluate how they might alter δ13C and δ15N values in tissues and, ultimately, whether archived DNA samples could be used for stable-isotope assay. Tissues included blood and pectoral muscle from quail (Coturnix coturnix japonica) and blood from sheep (Ovis aries). Preservation techniques for blood included freeze-drying (control), drying on precombusted glass-fibre filter paper, and storing in 70% ethanol, 10% buffered formalin, ABI lysis buffer, and Queen's lysis buffer. After 8 weeks, the use of both lysis buffers and formalin resulted in significant depletion of 13C and 15N in blood. Values for samples dried on glass-fibre filter paper or stored in 70% ethanol did not differ significantly from those for the control. Muscle tissue was freeze-dried (control) or stored in 70% ethanol, 10% buffered formalin, or DMSO solution. Both the DMSO and formalin treatments resulted in significant depletion of 13C and 15N compared with the control. Only the 70% ethanol treatment did not result in changes to either isotope ratio in muscle. Where freezing is not possible, we recommend that blood samples be dried or stored in 70% ethanol. Our study provides an estimate of isotopic correction factors that may be applied to tissues archived for DNA analysis or stored in formalin.

scholarly journals Stable nitrogen isotope analysis of amino acids as a new tool to clarify complex parasite–host interactions within food webs

Oikos ◽  
2021 ◽  
Author(s):  
Philip M. Riekenberg ◽  
Tijs Joling ◽  
Lonneke L. IJsseldijk ◽  
Andreas M. Waser ◽  
Marcel T. J. van der Meer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Food Webs ◽  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Stable Nitrogen Isotope ◽  
Host Interactions ◽  
Nitrogen Isotope Analysis

scholarly journals Stable carbon, oxygen, and nitrogen, isotope analysis of plants from a South Asian tropical forest: Implications for primatology

2017 ◽  
Vol 79 (6) ◽  
pp. e22656 ◽  
Author(s):  
Patrick Roberts ◽  
Scott A. Blumenthal ◽  
Wolfgang Dittus ◽  
Oshan Wedage ◽  
Julia A. Lee-Thorp
Keyword(s):  
Tropical Forest ◽  
South Asian ◽  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Stable Carbon ◽  
Nitrogen Isotope Analysis

scholarly journals Evaluation of two lipid removal methods for stable carbon and nitrogen isotope analysis in whale tissue

2020 ◽  
Vol 34 (18) ◽  
Author(s):  
Kerri J. Smith ◽  
Clive N. Trueman ◽  
Christine A.M. France ◽  
Markus J. Peterson
Keyword(s):  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Stable Carbon ◽  
Carbon And Nitrogen ◽  
Nitrogen Isotope Analysis

Stable carbon and nitrogen isotope analysis at Neolithic Çatalhöyük: evidence for human and animal diet and their relationship to households

2015 ◽  
Vol 57 ◽  
pp. 69-79 ◽  
Author(s):  
Jessica A. Pearson ◽  
Amy Bogaard ◽  
Mike Charles ◽  
Simon W. Hillson ◽  
Clark Spencer Larsen ◽  
...  
Keyword(s):  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Stable Carbon ◽  
Carbon And Nitrogen ◽  
Nitrogen Isotope Analysis ◽  
Animal Diet
Sign in / Sign up

Export Citation Format

Share Document