Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9- and 10-hydroxystearic acids

2002 ◽  
Vol 16 (9) ◽  
pp. 859-864 ◽  
Author(s):  
C. Bertucci ◽  
M. Hudaib ◽  
C. Boga ◽  
N. Calonghi ◽  
C. Cappadone ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Gas Chromatography ◽  
Quantitative Analysis ◽  
Mass Spectrometric ◽  
Cellular Lipid ◽  
Lipid Peroxidation Products

scholarly journals Products of Lipid Peroxidation as a Factor in the Toxic Effect of Silver Nanoparticles

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2460 ◽  
Author(s):  
Patrycja Paciorek ◽  
Mariusz Żuberek ◽  
Agnieszka Grzelak
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Silver Nanoparticles ◽  
Energy Metabolism ◽  
Cell Viability ◽  
Toxic Effect ◽  
Lipid Peroxides ◽  
Cellular Lipid ◽  
Amino Groups ◽  
Lipid Peroxidation Products

In our previous study we have shown that nanoparticles have different effects depending on the energy metabolism of the cell, which is an important factor in the context of oncology and diabetes. Here we assess the influence of AgNPs on cellular lipid components in varying glucose concentrations. To assess the effect of silver nanoparticles on cell lipids, we measured cell viability, the fluidity of the cell membranes, the content of amino groups in proteins, the level of lipid peroxidation products, the concentration of 4-hydroxynonenal (4-HNE), and the concentration of lipid peroxides. The obtained results show differences in the formation of lipid peroxidation products in cells exposed to oxidative stress induced by nanoparticles. In addition, we have shown that the metabolic state of the cell is a factor significantly affecting this process.

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