Removal of 3'-phosphate group by bacterial alkaline phosphatase improves oligonucleotide sequence coverage of RNase digestion products analyzed by collision-induced dissociation mass spectrometry

2011 ◽  
Vol 25 (23) ◽  
pp. 3609-3616 ◽  
Author(s):  
Kady L. Krivos ◽  
Balasubrahmanyam Addepalli ◽  
Patrick A. Limbach
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Phosphate Group ◽  
Collision Induced Dissociation ◽  
Sequence Coverage ◽  
Bacterial Alkaline Phosphatase ◽  
Rnase Digestion ◽  
Oligonucleotide Sequence

scholarly journals Dynamic consolidated bioprocessing for innovative lab-scale production of bacterial alkaline phosphatase from Bacillus paralicheniformis strain APSO

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Soad A. Abdelgalil ◽  
Nadia A. Soliman ◽  
Gaber A. Abo-Zaid ◽  
Yasser R. Abdel-Fattah
Keyword(s):  
Alkaline Phosphatase ◽  
Consolidated Bioprocessing ◽  
Black Liquor ◽  
Batch Cultivation ◽  
Efficient Production ◽  
Scale Production ◽  
Market Demand ◽  
Stirred Tank Bioreactor ◽  
Bacterial Alkaline Phosphatase ◽  
Bacillus Paralicheniformis

AbstractTo meet the present and forecasted market demand, bacterial alkaline phosphatase (ALP) production must be increased through innovative and efficient production strategies. Using sugarcane molasses and biogenic apatite as low-cost and easily available raw materials, this work demonstrates the scalability of ALP production from a newfound Bacillus paralicheniformis strain APSO isolated from a black liquor sample. Mathematical experimental designs including sequential Plackett–Burman followed by rotatable central composite designs were employed to select and optimize the concentrations of the statistically significant media components, which were determined to be molasses, (NH4)2NO3, and KCl. Batch cultivation in a 7-L stirred-tank bioreactor under uncontrolled pH conditions using the optimized medium resulted in a significant increase in both the volumetric and specific productivities of ALP; the alkaline phosphatase throughput 6650.9 U L−1, and µ = 0.0943 h−1; respectively, were obtained after 8 h that, ameliorated more than 20.96, 70.12 and 94 folds compared to basal media, PBD, and RCCD; respectively. However, neither the increased cell growth nor enhanced productivity of ALP was present under the pH-controlled batch cultivation. Overall, this work presents novel strategies for the statistical optimization and scaling up of bacterial ALP production using biogenic apatite.

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