Assessment of intracellular methotrexate and methotrexate-polyglutamate metabolite concentrations in erythrocytes by ultrafast matrix-assisted laser desorption/ionization triple quadrupole tandem mass spectrometry

2011 ◽  
Vol 25 (20) ◽  
pp. 3063-3070 ◽  
Author(s):  
Roland J. W. Meesters ◽  
Ethan Boer ◽  
Robert Jonge ◽  
Jan Lindemans ◽  
Theo M. Luider
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Tandem Mass ◽  
Triple Quadrupole ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Metabolite Concentrations ◽  
Methotrexate Polyglutamate ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Proteomic Analysis of Gill Mucus from Grass Carp Ctenopharyngodon idella

2013 ◽  
Vol 718-720 ◽  
pp. 288-292
Author(s):  
Guo Yong Liu ◽  
Xiao Tao Shi ◽  
Zhi Ying Tu ◽  
Li Ming Liu ◽  
Ying Ping Huang
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Laser Desorption ◽  
Ctenopharyngodon Idella ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Fish gills is part of the first-line defense in fish. The proteome in gill mucus of grass carp (Ctenopharyngodon idella) was examined in this study. Two-dimensional polyacrylamide gel electrophoresis, in combination with size exclusion chromatography, was used to resolve the proteins. Two-dimensional maps of gill mucus revealed 406 distinct spots with Coomassie blue staining, of which 256 protein spots were subjected to identification using matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF), matrix-assisted laser desorption / ionization tandem mass spectrometry (MALDI-MS / MS) and liquid chromatography tandem mass spectrometry (LC-MS / MS). A total of 99 spots representing 52 proteins were identified, including 19 immune-related molecules such as lectin, interlectin, transferrin, and proteasome. Their presence in gill mucus may imply the importance of non-specific immunity in gill mucus. The present study provides the first picture on proteome in mucus of a fish species, and thus the foundation for understanding quantitative and qualitative variation in proteins in the mucus.

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