scholarly journals Gas chromatographic mass spectrometric detection of dihydroxy fatty acids preserved in the ‘bound’ phase of organic residues of archaeological pottery vessels

2011 ◽  
Vol 25 (13) ◽  
pp. 1893-1898 ◽  
Author(s):  
Fabricio A. Hansel ◽  
Ian D. Bull ◽  
Richard P. Evershed
Keyword(s):  
Fatty Acids ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Organic Residues ◽  
Archaeological Pottery ◽  
Dihydroxy Fatty Acids ◽  
Chromatographic Mass

Gas chromatographic–mass spectrometric detection of 2- and 3-hydroxy fatty acids as methyl esters from soil, sediment and biofilm

2003 ◽  
Vol 783 (2) ◽  
pp. 443-451 ◽  
Author(s):  
M.M. Keinänen ◽  
L.K. Korhonen ◽  
P.J. Martikainen ◽  
T. Vartiainen ◽  
I.T. Miettinen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Methyl Esters ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Hydroxy Fatty Acids ◽  
Soil Sediment ◽  
Chromatographic Mass

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

Mass-Spectrometric Detection of Omega-Oxidation Products of Aliphatic Fatty Acids in Exhaled Breath

2017 ◽  
Vol 89 (19) ◽  
pp. 10329-10334 ◽  
Author(s):  
Martin Thomas Gaugg ◽  
Tobias Bruderer ◽  
Nora Nowak ◽  
Lara Eiffert ◽  
Pablo Martinez-Lozano Sinues ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Oxidation Products ◽  
Exhaled Breath

scholarly journals Improved Method for Gas Chromatographic–Mass Spectrometric Analysis of 1-13C-labeled Long-Chain Fatty Acids in Plasma Samples

2002 ◽  
Vol 48 (6) ◽  
pp. 906-912 ◽  
Author(s):  
José M Hernández-Pérez ◽  
Eduard Cabré ◽  
Lourdes Fluvià ◽  
Ágata Motos ◽  
Cruz Pastor ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Isotopic Ratio ◽  
Internal Standard ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Calibration Curves ◽  
Long Chain ◽  
Chromatographic Mass

Abstract Background: Gas chromatographic–mass spectrometric (GC/MS) tracking of stable-isotope-labeled substrates is useful in metabolic studies. However, GC/MS analysis of long-chain fatty acid methyl esters yields results that mostly depend on their concentration in the system. We describe a protocol aimed to obviate this and other drawbacks in plasma [1-13C]palmitic and [1-13C]oleic acid measurements. Methods: Lipoproteins were separated by sequential ultracentrifugation. Free or esterified heptadecanoic acid was used as internal standard. Fatty acids were derivatized to trimethylsilyl (TMS) esters. GC separation was in isothermal mode at 210 °C for 27 min. For both TMS-palmitate and TMS-oleate, M and [M + 1] signals were simultaneously acquired with a dual acquisition program in single-ion monitoring mode. Calibration mixtures containing increasing amounts of labeled fatty acids were prepared gravimetrically to construct calibration curves for isotopic enrichment. Likewise, five calibration curves (for increasing concentrations) were constructed for each fatty acid; this allowed selection of the most appropriate curve for the concentration in a plasma sample. Results: Oleic acid-TMS ester was clearly separated from that of its stereoisomer, elaidic acid. Within a 10-fold concentration range, the isotopic ratio was independent on the amount of the analyte in the sample, with a maximum uncertainty of 0.34% in terms of molar percent excess. In addition, the within- and between-day imprecision (CV) of the method was <1%. Conclusion: Results obtained with this method are independent of concentration and sufficiently precise for tracking 1-13C-labeled palmitic and oleic acids in biological samples

Gas chromatographic/mass spectrometric analysis of fatty acids found in aquatic algae

1988 ◽  
Vol 16 (1-12) ◽  
pp. 477-480 ◽  
Author(s):  
M. Cojocaru ◽  
M. Shlosberg ◽  
Z. Dubinsky ◽  
A. Finkel
Keyword(s):  
Fatty Acids ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Chromatographic Mass

2,3-Dihydroxy Fatty Acids-Containing Waxes in Storks (C iconiidae)

1996 ◽  
Vol 51 (9-10) ◽  
pp. 743-749 ◽  
Author(s):  
Jürgen Jacob ◽  
Gottfried Raab
Keyword(s):  
Fatty Acids ◽  
Mass Spectrometric ◽  
Hydroxy Fatty Acids ◽  
Uropygial Gland ◽  
Mass Spectrometric Fragmentation ◽  
Dihydroxy Fatty Acids ◽  
Fatty Acids And Alcohols

Uropygial gland secretions from five out of a total of seven species forming the genus Ciconia (family Ciconiidae; order Ciconiiformes) were found to consist of mixtures of monoester waxes, diester waxes, triester waxes, and triglycerides. Monoester waxes were composed of unbranched fatty acids and alcohols, whereas diester waxes derived from both 2- and 3-hydroxy fatty acids esterified with unbranched alcohols and fatty acids. Interestingly, triester waxes were also found deriving from either 2-hydroxy alkylmalonic acids or from erythro-2,3-dihydroxy fatty acids the latter of which have not yet been found in vertebrates so far. To compare the typical mass spectrometric fragmentation of this class of compounds erythro-2,3-dihydroxyhexadecanoic acid has been synthesized

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