Simultaneous qualitative and quantitative method using liquid chromatography selected reaction monitoring-triggered quantitation-enhanced data-dependent tandem mass spectrometry for the identification and classification of amphetamine-type stimulant abuse

2010 ◽  
Vol 24 (21) ◽  
pp. 3139-3145 ◽  
Author(s):  
Sang Kyu Lee ◽  
So-Hee Kim ◽  
Ho Jun Kim ◽  
Hye Hyun Yoo ◽  
Oh Seung Kwon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Quantitative Method ◽  
Selected Reaction Monitoring ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Qualitative And Quantitative ◽  
Stimulant Abuse

Assessment of semi-permanent hair dyes in wash water from beauty salons by liquid chromatography-tandem mass spectrometry-selected reaction monitoring (LC-MS/MS-SRM)

2020 ◽  
Vol 12 (44) ◽  
pp. 5415-5423
Author(s):  
Jefferson Honorio Franco ◽  
Bianca F. da Silva ◽  
Maria V. B. Zanoni
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Wash Water ◽  
Selected Reaction Monitoring ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Hair Dyes ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Herein, we present an approach for the quantification of semi-permanent hair dyes in wash water samples released during washing of dyed hair employing a liquid chromatography-tandem mass spectrometry-selected reaction monitoring (LC-MS/MS-SRM).

scholarly journals Pitfalls and Prevention Strategies for Liquid Chromatography-Tandem Mass Spectrometry in the Selected Reaction– Monitoring Mode for Drug Analysis

2008 ◽  
Vol 54 (9) ◽  
pp. 1519-1527 ◽  
Author(s):  
François-Ludovic Sauvage ◽  
Jean-Michel Gaulier ◽  
Gérard Lachâtre ◽  
Pierre Marquet
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Relative Abundance ◽  
False Positive ◽  
Screening Method ◽  
Selected Reaction Monitoring ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Positive Results

Abstract Background: We observed cases of false-positive results with the use of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Different LC-MS/MS techniques that use the selected reaction-monitoring mode, routinely employed for the analysis and quantification of drugs and toxic compounds in biological matrices, were involved in the false-positive and potentially false-positive results obtained. We sought to analyze the causes of and solutions to this problem. Methods: We used a previously reported LC-MS/MS general unknown screening method, as well as manual spectral investigation in 1 case, to perform verification and identification of interfering compounds. Results: We observed that false-positive results involved: a metabolite of zolpidem that might have been mistaken for lysergic acid diethylamide, benzoylecgonine mistaken for atropine, and clomipramine and 3 phenothiazines that share several common ion transitions. Conclusions: To prevent problems such as those we experienced, we recommend the use of stable-isotope internal standards when possible, relative retention times, 2 transitions or more per compound when possible, and acceptable relative abundance ratios between transitions, with an experience-based tolerance of ±15% for transitions with a relative abundance >10% and with an extension to ±25% for transitions <10% when the concentration is at the limit of quantification. A powerful general unknown screening procedure can help to confirm suspected interferences. Our results indicate that the specificity of screening procedures is questionable for LC-MS/MS analyses performed in the selected reaction-monitoring mode and involving a large number of compounds with only 1 transition per compound.

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