Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in Flos Lonicerae Japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry

2009 ◽  
Vol 23 (19) ◽  
pp. 3227-3242 ◽  
Author(s):  
Lian-Wen Qi ◽  
Chun-Yun Chen ◽  
Ping Li
Keyword(s):  
Iridoid Glycosides ◽  
Diode Array ◽  
Diode Array Detection ◽  
Chromatography Method ◽  
Flight Mass Spectrometry ◽  
Flos Lonicerae ◽  
Array Detection ◽  
Liquid Chromatography Method ◽  
Fast Liquid Chromatography ◽  
Flos Lonicerae Japonicae

scholarly journals Identification and Content of Astaxanthin and Its Esters from Microalgae Haematococcus pluvialis by HPLC-DAD and LC-QTOF-MS after Extraction with Various Solvents

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2413
Author(s):  
Biljana Todorović ◽  
Veno Jaša Grujić ◽  
Andreja Urbanek Krajnc ◽  
Roman Kranvogl ◽  
Jana Ambrožič-Dolinšek
Keyword(s):  
High Performance ◽  
Haematococcus Pluvialis ◽  
Methyl Tert Butyl Ether ◽  
Diode Array ◽  
Diode Array Detection ◽  
Butyl Ether ◽  
Flight Mass Spectrometry ◽  
Green Microalga ◽  
Array Detection ◽  
Long Chain

Haematococcus pluvialis, a unicellular green microalga that produces a secondary metabolite under stress conditions, bears one of the most potent antioxidants, namely xanthophyll astaxanthin. The aim of our study was to determine the content of astaxanthin and its esterified forms using three different solvents—methyl tert-butyl ether (MTBE), hexane isopropanol (HEX -IPA) and acetone (ACE)—and to identify them by using high performance liquid chromatography coupled with diode array detection and the quadrupole time-of-flight mass spectrometry (HPLC-DAD and LC-QTOF-MS) technique. We identified eleven astaxanthin monoesters, which accounted for 78.8% of the total astaxanthin pool, six astaxanthin diesters (20.5% of total), while free astaxanthin represented the smallest fraction (0.7%). Astaxanthin monoesters (C16:2, C16:1, C16:0), which were the major bioactive compounds in the H. pluvialis samples studied, ranged from 10.2 to 11.8 mg g−1 DW. Astaxanthin diesters (C18:4/C18:3, C18:1/C18:3) were detected in the range between 2.3 and 2.6 mg g−1 DW. All three solvents were found to be effective for extraction, but MTBE and hexane-isopropanol extracted the greatest amount of free bioactive astaxanthin. Furthermore, MTBE extracted more low-chain astaxanthin monoesters (C16), and hexane-isopropanol extracted more long-chain monoesters (C18 and above) and more diesters. We can conclude that MTBE is the solvent of choice for the extraction of monoesters and hexane-isopropanol for diesters.

Sign in / Sign up

Export Citation Format

Share Document