Compatibility of electron ionization and soft ionization methods in gas chromatography/orthogonal time-of-flight mass spectrometry

2009 ◽  
Vol 23 (14) ◽  
pp. 2181-2189 ◽  
Author(s):  
Leila Hejazi ◽  
Diako Ebrahimi ◽  
D. Brynn Hibbert ◽  
Michael Guilhaus
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Time Of Flight ◽  
Electron Ionization ◽  
Soft Ionization ◽  
Flight Mass Spectrometry ◽  
Ionization Methods

Exploring the extra-virgin olive oil volatilome by adding extra dimensions to comprehensive two-dimensional gas chromatography and time of flight mass spectrometry featuring tandem ionization: validation of ripening markers in headspace linearity conditions

2020 ◽  
Author(s):  
Federico Stilo ◽  
Erica Liberto ◽  
Stephen E Reichenbach ◽  
Qingping Tao ◽  
Carlo Bicchi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Time Of Flight ◽  
Extra Virgin Olive Oil ◽  
Two Dimensional ◽  
Soft Ionization ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Abstract Background Comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) is the most informative analytical approach for chemical characterization of the complex food volatilome. Key analytical features include: separation power and resolution enhancement; improved sensitivity; and structured separation patterns from chemically correlated analytes. Objective In this study, we explore the complex extra-virgin olive oil volatilome by combining head-space (HS) solid-phase microextraction, applied under HS linearity conditions to GC×GC-TOF MS featuring hard and soft ionization in tandem. Methods Multiple analytical dimensions are combined in a single run and evaluated in terms of: chemical dimensionality; method absolute and relative sensitivity; identification reliability provided by spectral signatures acquired at 70 and 12 eV; and dynamic and linear range of response provided by soft ionization. Results Method effectiveness is validated on a sample set of oils from Picual olives at different ripening stages. Ripening markers (3,4-diethyl-1,5-hexadiene (RS/SR), 3,4-diethyl-1,5-hexadiene (meso), (5Z)-3-ethyl-1,5-octadiene, (5E)-3-ethyl-1,5-octadiene, (E,Z)-3,7-decadiene and (E,E)-3,7-decadiene, (Z)-2-hexenal, (Z)-3-hexenal and (Z)-3-hexenal, (E)-2-pentenal, (Z)-2-pentenal, 1-pentanol, 1-penten-3-ol, 3-pentanone, and 1-penten-3-one) and quality indexes ((Z)-3-Hexenal/Nonanal, (Z)-3-Hexenal/Octane, (E)-2-Pentenal/Nonanal, and (E)-2-Pentenal/Octane) are confirmed for their validity in HS linearity conditions. Conclusions : For the complex olive oil volatilome, the proposed approach offers concrete advantages for the validation of the informative role of existing analytes while suggesting new potential markers to be studied in larger sample sets. Highlights The accurate fingerprinting of volatiles by HS-SPME operating in HS linearity conditions followed by GC×GC-TOF MS featuring tandem ionization gives the opportunity to improve the quality of analytical data and reliability of results.

Analysis of synthetic canine training aids by comprehensive two-dimensional gas chromatography–time of flight mass spectrometry

2012 ◽  
Vol 1255 ◽  
pp. 202-206 ◽  
Author(s):  
Sonja Stadler ◽  
Pierre-Hugues Stefanuto ◽  
Jonathan D. Byer ◽  
Michał Brokl ◽  
Shari Forbes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Time Of Flight ◽  
Two Dimensional ◽  
Flight Mass Spectrometry ◽  
Training Aids ◽  
Canine Training
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