scholarly journals High-resolution extracted ion chromatography, a new tool for metabolomics and lipidomics using a second-generation orbitrap mass spectrometer

2009 ◽  
Vol 23 (10) ◽  
pp. 1411-1418 ◽  
Author(s):  
Albert Koulman ◽  
Gary Woffendin ◽  
Vinod K. Narayana ◽  
Helen Welchman ◽  
Catharina Crone ◽  
...  
Keyword(s):  
High Resolution ◽  
Mass Spectrometer ◽  
Ion Chromatography ◽  
Second Generation ◽  
Orbitrap Mass Spectrometer

Characterization of hydrocoptisonine metabolites in human liver microsomes using a high-resolution quadrupole-orbitrap mass spectrometer

Xenobiotica ◽  
2020 ◽  
Vol 50 (12) ◽  
pp. 1423-1433
Author(s):  
Su Min Choi ◽  
Younah Kim ◽  
Jaeick Lee ◽  
Ju-Hyun Kim ◽  
Taeho Lee ◽  
...  
Keyword(s):  
High Resolution ◽  
Mass Spectrometer ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Orbitrap Mass Spectrometer

Surface-Induced Dissociation of Noncovalent Protein Complexes in an Extended Mass Range Orbitrap Mass Spectrometer

2018 ◽  
Author(s):  
Zachary VanAernum ◽  
Joshua D. Gilbert ◽  
Mikhail E. Belov ◽  
Alexander A. Makarov ◽  
Stevan R. Horning ◽  
...  
Keyword(s):  
High Resolution ◽  
Mass Spectrometer ◽  
Protein Complex ◽  
Protein Complexes ◽  
Mass Range ◽  
Ion Source ◽  
High Mass ◽  
Ligand Complex ◽  
Surface Induced Dissociation ◽  
Orbitrap Mass Spectrometer

Herein we demonstrate the first adaptation of surface-induced dissociation in a modified high-mass range, high-resolution Orbitrap mass spectrometer. The SID device was designed to be installed in the Q-Exactive series of Orbitrap mass spectrometers with minimal disruption of standard functions. The performance of the SID-Orbitrap instrument has been demonstrated with several protein complex and ligand-bound protein complex systems ranging from 53 to 336 kDa. We also address the effect of ion source temperature on native protein-ligand complex ions as assessed by SID. Results are consistent with previous findings on quadrupole time-of-flight instruments and suggest that SID coupled to high-resolution MS is well-suited to provide information on the interface interactions within protein complexes and ligand-bound protein complexes. <br>

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