Gas-phase fragmentation study of a novel series of synthetic 2-oxo-2H -benzopyrano[2,3-d ]pyrimidine derivatives using electrospray ionization tandem mass spectroscopy measured with a quadrupole orthogonal time-of-flight hybrid instrument

2008 ◽  
Vol 23 (2) ◽  
pp. 267-281 ◽  
Author(s):  
Hamida Turki ◽  
Benoit Genestie ◽  
Rachid El Gharbi ◽  
Sameh El Boussiry ◽  
Joseph Banoub
Keyword(s):  
Electrospray Ionization ◽  
Gas Phase ◽  
Mass Spectroscopy ◽  
Time Of Flight ◽  
Tandem Mass ◽  
Pyrimidine Derivatives ◽  
Tandem Mass Spectroscopy ◽  
Gas Phase Fragmentation

scholarly journals Medicinal use of Sceletium: Characterization of Phytochemical Components of Sceletium Plant Species using HPLC with UV and Electrospray Ionization – Tandem Mass Spectroscopy

2015 ◽  
Vol 18 (4) ◽  
pp. 414 ◽  
Author(s):  
Srinivas Patnala ◽  
Isadore Kanfer
Keyword(s):  
Electrospray Ionization ◽  
Plant Species ◽  
Mass Spectroscopy ◽  
Uv Detection ◽  
Tandem Mass ◽  
Esi Ms ◽  
Ms Detection ◽  
Wide Range ◽  
Tandem Mass Spectroscopy ◽  
Phytochemical Components

Purpose. Sceletium plants have been used for its medicinal properties for centuries. However, there is a wide range of Sceletium plant species in which various alkaloidal components such as ∆7mesembrenone, mesembrenol, mesembranol, mesembrenone, mesembrine hydrochloride, epimesembranol and, sceletium A4 differ between species. Hence, to ensure the quality of Sceletium products used as a medicine, it is imperative to identify the appropriate species using both botanical and chemical methods. The chemical approach to identify and characterize the phytochemical composition of a particular species facilitates the choice of species that will provide the purported therapeutic outcome.  Hence, specific analytical methods to identify relevant constituents from complex matrices are necessary. Although HPLC-UV detection is commonly used to identify and estimate phytochemical content of medicinal plants, use of mass spectroscopy (MS) and tandem mass spectroscopy (MS/MS) can unequivocally confirm their presence/absence based on characteristic ions and fragmentation patterns. Methods. The various alkaloidal components were characterized by electrospray ionization (ESI) MS and MS/MS using an ionizing medium of 0.1% ammonium hydroxide in water mixed with acetonitrile. Compounds were purified and characterized for use as reference standards to identify the relevant alkaloidal constituents of several Sceletium plant species using HPLC with on-line UV-MS detection. Results. ESI-MS provided the [M+H]+ ions with respective m/z values that related to the respective molecular weights 287, 289, 291, 287, 289, 324 and 291 for the above mentioned alkaloids, whereas, ESI MS/MS provided the characteristic fragment ions to confirm the structural identity of the individual alkaloids and subsequently used to  confirm the presence and/or absence of specific alkaloids in various Sceletium plant samples. Conclusions. Whilst HPLC-UV detection has been a widely-used conventional analytical technique for both qualitative and quantitative analyses, the results highlight the necessity of ESI-MS detection to avoid erroneous identification of phytochemical components, particularly with mesembrine-type compounds which have closely related chemical structures. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Gas-phase fragmentation of γ-lactone derivatives by electrospray ionization tandem mass spectrometry

2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Antonio E. M. Crotti ◽  
Erika S. Bronze-Uhle ◽  
Paulo G. B. D. Nascimento ◽  
Paulo M. Donate ◽  
Sérgio E. Galembeck ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Electrospray Ionization ◽  
Gas Phase ◽  
Tandem Mass ◽  
Gas Phase Fragmentation ◽  
Ionization Tandem Mass Spectrometry
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