Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

2008 ◽  
Vol 22 (18) ◽  
pp. 2825-2834 ◽  
Author(s):  
Kim-Chung Lee ◽  
Wan Chan ◽  
Zhitao Liang ◽  
Ning Liu ◽  
Zhongzhen Zhao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Trap ◽  
Screening Method ◽  
Negative Ion ◽  
Rapid Screening ◽  
Ion Trap Mass Spectrometry ◽  
Chinese Medicinal Herbs ◽  
Rapid Screening Method ◽  
Negative Ion Mode

Liquid Chromatography/Quadrupole Ion Trap/Time-of-Flight Determination of the Efficacy of Drug Test Kits for Rapid Screening of Food

2008 ◽  
Vol 71 (5) ◽  
pp. 1007-1014 ◽  
Author(s):  
JESSICA B. ZUCKSCHWERDT ◽  
CHRISTOPHER E. NIXON ◽  
FREDERIC L. CINER ◽  
TIMOTHY R. CROLEY
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Trap ◽  
Solid Phase ◽  
Screening Method ◽  
Time Of Flight ◽  
Internal Standard ◽  
Quadrupole Ion Trap ◽  
Rapid Screening ◽  
Food Matrices

The goal of this study was to evaluate the plausibility and accuracy of commercially available on-site immunoassay urinalysis kits for the screening of compounds of interest within food matrices. In conjunction with this study, a sensitive, robust, and reproducible analytical method, utilizing solid-phase extraction liquid chromatography/quadrupole ion trap/time-of-flight mass spectrometry for confirmation analysis, was developed. The food matrices analyzed were tomato juice, apple juice, milk, beer, white wine, ground beef, powdered milk, and all-purpose flour. Compounds fortified into the food matrices included heroin, phencyclidine, cocaine, benzoylecgonine, methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, imipramine, doxepin, nitrazepam, diazepam, oxazepam, temazepam, alprazolam, flunitrazepam, clonazepam, and lorazepam. Standard curves were prepared for each matrix from 10 to 500 ng/ml for each analyzed compound. All liquid chromatography/tandem mass spectrometry samples were fortified with 20 μl of deuterated internal standard at 90 ng/ml. Quality control standards were prepared at 20 and 400 ng/ml, and >90% were within 2 SD of the mean for each analyte. The test kits were found to produce up to 85% of the expected results based on concentration levels of adulterants (i-Screen in milk). This study shows that lateral-flow immunoassay test kits are plausible as a rapid, accurate, and reliable screening method in the event of adulteration of the food supply.

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