Top-down approach of completely sequencing a 4.9 kDa recombinant peptide using quadrupole time-of-flight mass spectrometry

2006 ◽  
Vol 20 (24) ◽  
pp. 3747-3754 ◽  
Author(s):  
Putchen Dakshinamoorthy Deepalakshmi
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Top Down ◽  
Recombinant Peptide ◽  
Flight Mass Spectrometry

Top-down N-terminal sequencing of Immunoglobulin subunits with electrospray ionization time of flight mass spectrometry

2009 ◽  
Vol 384 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Da Ren ◽  
Gary D. Pipes ◽  
David Hambly ◽  
Pavel V. Bondarenko ◽  
Michael J. Treuheit ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Time Of Flight ◽  
Top Down ◽  
Ionization Time ◽  
Flight Mass Spectrometry

Infrared Multiphoton Dissociation in Quadrupole Time-of-Flight Mass Spectrometry:  Top-Down Characterization of Proteins

2006 ◽  
Vol 78 (13) ◽  
pp. 4572-4577 ◽  
Author(s):  
Serguei A. Raspopov ◽  
Ayman El-Faramawy ◽  
Bruce A. Thomson ◽  
K. W. Michael Siu
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Infrared Multiphoton Dissociation ◽  
Top Down ◽  
Flight Mass Spectrometry ◽  
Multiphoton Dissociation

Characterization of Recombinant Protein Mutants by Top-down Sequencing Using Quadrupole Time-of-Flight Mass Spectrometry

2009 ◽  
Vol 15 (5) ◽  
pp. 641-649 ◽  
Author(s):  
Deepalakshmi Dakshinamoorthy Putchen
Keyword(s):  
Mass Spectrometry ◽  
Recombinant Proteins ◽  
Acyl Carrier Protein ◽  
Time Of Flight ◽  
Biologically Active ◽  
Fragmentation Pattern ◽  
Post Translational Modification ◽  
Top Down ◽  
E Coli ◽  
Flight Mass Spectrometry

Top-down sequencing using quadrupole time-of-flight mass spectrometry is used as a direct way of locating the mutated sites of recombinant proteins and post-translational modification in a protein. Several mutants of barstar, expressed in E. coli, were confirmed by analyzing the fragmentation pattern of mutants. A contaminant protein, that appeared while purifying mutants of barstar, was identified as an acyl carrier protein from E. coli with a post-translational modification on serine residue, indicating that the protein was biologically active. A mutant of ribosomal protein S6 has been characterized with neutral loss of ammonia at the N-terminal region of the protein. The power of the top-down approach in characterizing the mutants of recombinant proteins has been demonstrated.

scholarly journals Top-down analysis of 30–80 kDa proteins by electron transfer dissociation time-of-flight mass spectrometry

2013 ◽  
Vol 405 (26) ◽  
pp. 8505-8514 ◽  
Author(s):  
Luca Fornelli ◽  
Julien Parra ◽  
Ralf Hartmer ◽  
Carsten Stoermer ◽  
Markus Lubeck ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Electron Transfer Dissociation ◽  
Time Of Flight ◽  
Top Down ◽  
Flight Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document