Use of hydrogen/deuterium exchange mass spectrometry and mutagenesis as a tool to identify the binding region of inhibitors targeting the human mitotic kinesin Eg5

Sébastien Brier; David Lemaire; Salvatore DeBonis; Frank Kozielski; Eric Forest

doi:10.1002/rcm.2329

Use of hydrogen/deuterium exchange mass spectrometry and mutagenesis as a tool to identify the binding region of inhibitors targeting the human mitotic kinesin Eg5

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.2329 ◽

2006 ◽

Vol 20 (3) ◽

pp. 456-462 ◽

Cited By ~ 24

Author(s):

Sébastien Brier ◽

David Lemaire ◽

Salvatore DeBonis ◽

Frank Kozielski ◽

Eric Forest

Keyword(s):

Mass Spectrometry ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Binding Region ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Kinesin Eg5 ◽

Mitotic Kinesin Eg5

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Localizing the Membrane Binding Region of Group VIA Ca2+-independent Phospholipase A2Using Peptide Amide Hydrogen/Deuterium Exchange Mass Spectrometry

Journal of Biological Chemistry ◽

10.1074/jbc.m109.021857 ◽

2009 ◽

Vol 284 (35) ◽

pp. 23652-23661 ◽

Cited By ~ 49

Author(s):

Yuan-Hao Hsu ◽

John E. Burke ◽

Sheng Li ◽

Virgil L. Woods ◽

Edward A. Dennis

Keyword(s):

Mass Spectrometry ◽

Membrane Binding ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Amide Hydrogen ◽

Binding Region ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Peptide Amide

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Detection of structural and conformational changes in ALS-causing mutant profilin-1 with hydrogen/deuterium exchange mass spectrometry and bioinformatics techniques

Metabolic Brain Disease ◽

10.1007/s11011-021-00763-y ◽

2021 ◽

Author(s):

Ahmad Shahir Sadr ◽

Zahra Abdollahpour ◽

Atousa Aliahmadi ◽

Changiz Eslahchi ◽

Mina Nekouei ◽

...

Keyword(s):

Mass Spectrometry ◽

Conformational Changes ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Design and Validation of In-Source Atmospheric Pressure Photoionization Hydrogen/Deuterium Exchange Mass Spectrometry with Continuous Feeding of D2O

Journal of the American Society for Mass Spectrometry ◽

10.1007/s13361-017-1831-8 ◽

2017 ◽

Vol 29 (1) ◽

pp. 85-94 ◽

Cited By ~ 4

Author(s):

Thamina Acter ◽

Seulgidaun Lee ◽

Eunji Cho ◽

Maeng-Joon Jung ◽

Sunghwan Kim

Keyword(s):

Mass Spectrometry ◽

Atmospheric Pressure ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Atmospheric Pressure Photoionization ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Continuous Feeding

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Different conformational dynamics of various active states of β-arrestin1 analyzed by hydrogen/deuterium exchange mass spectrometry

Journal of Structural Biology ◽

10.1016/j.jsb.2015.04.006 ◽

2015 ◽

Vol 190 (2) ◽

pp. 250-259 ◽

Cited By ~ 11

Author(s):

Dong Kyun Kim ◽

Youngjoo Yun ◽

Hee Ryung Kim ◽

Min-Duk Seo ◽

Ka Young Chung

Keyword(s):

Mass Spectrometry ◽

Conformational Dynamics ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Hydrogen deuterium exchange mass spectrometry in biopharmaceutical discovery and development – A review

Analytica Chimica Acta ◽

10.1016/j.aca.2016.08.006 ◽

2016 ◽

Vol 940 ◽

pp. 8-20 ◽

Cited By ~ 36

Author(s):

Bin Deng ◽

Cristina Lento ◽

Derek J. Wilson

Keyword(s):

Mass Spectrometry ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Hydrogen-Deuterium Exchange Mass Spectrometry of Phosphorylation Induced Changes in Smooth Muscle Myosin

Biophysical Journal ◽

10.1016/j.bpj.2012.11.2501 ◽

2013 ◽

Vol 104 (2) ◽

pp. 452a

Author(s):

K. Ilker Sen ◽

Brian D. Haldeman ◽

Richard Brizendine ◽

Roger Mercer ◽

Christine R. Cremo ◽

...

Keyword(s):

Mass Spectrometry ◽

Smooth Muscle ◽

Smooth Muscle Myosin ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Induced Changes ◽

Muscle Myosin

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Deglycosylation induces extensive dynamics changes in α-amylase revealed by hydrogen/deuterium exchange mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.6732 ◽

2013 ◽

Vol 27 (23) ◽

pp. 2625-2630 ◽

Cited By ~ 4

Author(s):

Chengjie Ji ◽

Gary Wei

Keyword(s):

Mass Spectrometry ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Research advances in hydrogen–deuterium exchange mass spectrometry for protein epitope mapping

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-020-03091-9 ◽

2021 ◽

Author(s):

Haofeng Sun ◽

Lingyun Ma ◽

Leyu Wang ◽

Peng Xiao ◽

Hongmei Li ◽

...

Keyword(s):

Mass Spectrometry ◽

Epitope Mapping ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Moderated Test Statistics to Detect Differential Deuteration in Hydrogen/Deuterium Exchange Mass Spectrometry Experiments

Analytical Chemistry ◽

10.1021/acs.analchem.1c02346 ◽

2021 ◽

Author(s):

Jürgen Claesen ◽

Srinath Krishnamurthy ◽

Andy M. Lau ◽

Anastassios Economou

Keyword(s):

Mass Spectrometry ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Test Statistics ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Cover Picture: Identification of Pharmacological Chaperones for Gaucher Disease and Characterization of Their Effects on β-Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry (ChemBioChem 16/2008)

ChemBioChem ◽

10.1002/cbic.200890061 ◽

2008 ◽

Vol 9 (16) ◽

pp. 2549-2549

Author(s):

Michael B. Tropak ◽

Gregory J. Kornhaber ◽

Brigitte A. Rigat ◽

Gustavo H. Maegawa ◽

Justin D. Buttner ◽

...

Keyword(s):

Mass Spectrometry ◽

Gaucher Disease ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Pharmacological Chaperones ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Picture Identification

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