Enhancement of matrix-assisted laser desorption/ionization photodissociation tandem time-of-flight mass spectra; spectral reduction and cleanup of isotopomeric contamination

2005 ◽  
Vol 19 (18) ◽  
pp. 2481-2487 ◽  
Author(s):  
Jeong Hee Moon ◽  
So Hee Yoon ◽  
Myung Soo Kim
Keyword(s):  
Mass Spectra ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Spectral Reduction ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals A new method to analyze matrix-assisted laser desorption/ionization time-of-flight peptide profiling mass spectra

2005 ◽  
Vol 19 (7) ◽  
pp. 865-870 ◽  
Author(s):  
Lennard J. Dekker ◽  
Johannes C. Dalebout ◽  
Ivar Siccama ◽  
Guido Jenster ◽  
Peter A. Sillevis Smitt ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Time Of Flight ◽  
Laser Desorption ◽  
New Method ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Rapid Characterization of Spores of Bacillus cereusGroup Bacteria by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry

2000 ◽  
Vol 66 (9) ◽  
pp. 3828-3834 ◽  
Author(s):  
Victor Ryzhov ◽  
Yetrib Hathout ◽  
Catherine Fenselau
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectra ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Rapid Characterization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Matrix-assisted laser desorption-ionization (MALDI) time-of-flight mass spectrometry was used to characterize the spores of 14 microorganisms of the Bacillus cereus group. This group includes the four Bacillus species B. anthracis, B. cereus, B. mycoides, andB. thuringiensis. MALDI mass spectra obtained from whole bacterial spores showed many similarities between the species, except for B. mycoides. At the same time, unique mass spectra could be obtained for the different B. cereus and B. thuringiensis strains, allowing for differentiation at the strain level. To increase the number of detectable biomarkers in the usually peak-poor MALDI spectra of spores, the spores were treated by corona plasma discharge (CPD) or sonicated prior to MALDI analysis. Spectra of sonicated or CPD-treated spores displayed an ensemble of biomarkers common for B. cereus group bacteria. Based on the spectra available, these biomarkers differentiate B. cereus group spores from those of Bacillus subtilis andBacillus globigii. The effect of growth medium on MALDI spectra of spores was also explored.

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