Effect of insulin on intracellular ph as observed by 31p NMR spectroscopy

2009 ◽  
Vol 18 (S7) ◽  
pp. 83-92 ◽  
Author(s):  
Richard D. Moore ◽  
Raj K. Gupta
Keyword(s):  
Nmr Spectroscopy ◽  
Intracellular Ph ◽  
31P Nmr ◽  
31P Nmr Spectroscopy

31P-NMR spectroscopy of isolated perfused rat lung

1993 ◽  
Vol 74 (4) ◽  
pp. 1549-1554 ◽  
Author(s):  
Y. Hayashi ◽  
T. Inubushi ◽  
S. Nioka ◽  
R. E. Forster
Keyword(s):  
Nmr Spectroscopy ◽  
Intracellular Ph ◽  
31P Nmr ◽  
Peak Height ◽  
31P Nmr Spectroscopy ◽  
Line Broadening ◽  
Membrane Phospholipids ◽  
The Difference ◽  
Inflated Lung

We obtained 202.5-MHz 31P-nuclear magnetic resonance (NMR) spectra of isolated perfused rat lungs, degassed and inflated, and of lung extract. The spectra included those of ATP, ADP, phosphocreatine (PCr), inorganic phosphate (Pi), phosphomonoesters, phosphodiesters, and a broad component due to the membrane phospholipids. The line width at one-half peak height for beta-ATP was 1.0 ppm for the degassed lung and 1.2 ppm for the inflated lung. This suggests that the air-water interfaces in inflated lung, which produce proton NMR line broadening, do not act prominently in 31P-NMR spectroscopy. In a degassed lung, when perfusion was stopped for up to 30 min, PCr and ATP peaks decreased progressively with time while Pi and phosphomonoester peaks increased. On return of flow, these changes reversed. The intracellular pH values calculated from the difference in magnetic field between PCr and Pi peaks of inflated and degassed lungs were 7.16 +/- 0.10 (SD; n = 4) and 6.99 +/- 0.10 (n = 4), respectively. The change of intracellular pH caused by 30 min of ischemia was -0.2 pH units. Our findings indicate that air-water interfaces should not broaden lung 31P peaks in vivo.

The Intracellular pH of a Molluscan Smooth Muscle During a Contraction-Catch-Relaxation Cycle Estimated by the Distribution of [14C]DMO and by 31P-NMR Spectroscopy

1990 ◽  
Vol 150 (1) ◽  
pp. 81-93 ◽  
Author(s):  
J. ZANGE ◽  
H. O. PÖRTNER ◽  
A. W. JANS ◽  
M. K. GREESHABER
Keyword(s):  
Smooth Muscle ◽  
Nmr Spectroscopy ◽  
Cyclic Amp ◽  
Intracellular Ph ◽  
31P Nmr ◽  
Retractor Muscle ◽  
31P Nmr Spectroscopy ◽  
Relaxation Cycle ◽  
Anterior Byssus Retractor Muscle ◽  
Molluscan Smooth Muscle

The intracellular pH (pHi) of the anterior byssus retractor muscle (ABRM) of Mytilus edulis L. was estimated during rest and during a contraction-catch-relaxation cycle by the distribution of [14C]DMO and by 31P-NMR spectroscopy. The pHi of resting muscles was found to be nearly the same whether it was determined with DMO (7.41±0.06, N=5l) or by 31P-NMR spectroscopy (7.44±0.02, N=5). During catch the pHi was not significantly different from its value at rest. Serotonin (10−5moll−1) induced a rapid relaxation and a significant increase in pHi (DMO: 7.56±0.05, N=5; 31P-NMR: 7.57±0.02, N=5). In resting ABRM serotonin (≥10−6moll−1) also induced an alkalosis. It was shown that cyclic AMP, the second messenger of serotonin, elicited an increase of pHi. Dopamine (10−5moll−1) did not cause an increase in either cyclic AMP levels or pHi. The rates by which tonically contracted ABRM loaded at 0.1 N were stretched were significantly increased by an alkalosis elicited by the addition of 10 or 20mmoll−1 methylamine. Acidosis elicited by lO mmoll−1 DMO caused a significant decrease in the rate of relaxation.

scholarly journals Effects of vitamin E, C phosphate on intracellular pH and energy metabolism of the ischemic brain studied by 31P NMR spectroscopy

1989 ◽  
Vol 49 ◽  
pp. 130
Author(s):  
Yoshikazu Kurebayashi ◽  
Hiroaki Naritomi ◽  
Masahiro Sasaki ◽  
Masaru Kanashiro ◽  
Tohru Sawada
Keyword(s):  
Vitamin E ◽  
Energy Metabolism ◽  
Nmr Spectroscopy ◽  
Intracellular Ph ◽  
31P Nmr ◽  
31P Nmr Spectroscopy ◽  
Ischemic Brain

Imaging of phosphoenergetic state and intracellular pH in human calf muscles after exercise by 31P NMR spectroscopy

1994 ◽  
Vol 12 (7) ◽  
pp. 1121-1126 ◽  
Author(s):  
Shigehiro Morikawa ◽  
Toshiro Inubushi ◽  
Kouichi Kito ◽  
Ryoko Tabata
Keyword(s):  
Nmr Spectroscopy ◽  
Intracellular Ph ◽  
31P Nmr ◽  
31P Nmr Spectroscopy ◽  
Human Calf ◽  
Calf Muscles

Regulation of intracellular pH in LLC-PK1 cells studied using 31P-NMR spectroscopy

1987 ◽  
Vol 927 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Arnold W.H. Jans ◽  
Kurt Amsler ◽  
Bernhard Griewel ◽  
Rolf K.H. Kinne
Keyword(s):  
Nmr Spectroscopy ◽  
Intracellular Ph ◽  
31P Nmr ◽  
31P Nmr Spectroscopy

Quantitative Determination of Krill Oil Composition by ATR-FTIR, NIR, and 31P NMR Spectroscopy

2020 ◽  
Author(s):  
Ashraf Ismail ◽  
Sanaz Molaye Moghaddam ◽  
Jean-Pierre MetabanzoulouSarya Aziz ◽  
Jacqueline Sedman ◽  
Mazen Bahadi
Keyword(s):  
Nmr Spectroscopy ◽  
Quantitative Determination ◽  
31P Nmr ◽  
31P Nmr Spectroscopy ◽  
Krill Oil ◽  
Oil Composition

Enzymatic decomposition of a locked nucleoside phosphoramidate monitored by 31P NMR spectroscopy

2014 ◽  
Author(s):  
Eliška Procházková ◽  
Hubert Hřebabecký ◽  
Radim Nencka ◽  
Martin Dračínský
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
31P Nmr Spectroscopy
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