Effect of novel bottle inner surface coatings made with polylactic acid and plant extracts on the physiological status of Pseudomonas aeruginosa in real liquid foods using flow cytometry

2021 ◽  
Author(s):  
Saliha Oussaid ◽  
Khodir Madani ◽  
Karim Houali ◽  
Ismael Marcet ◽  
Tahar Amrouche ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Pseudomonas Aeruginosa ◽  
Polylactic Acid ◽  
Plant Extracts ◽  
Surface Coatings ◽  
Physiological Status ◽  
Inner Surface ◽  
Liquid Foods

scholarly journals Flow cytometric measurements as a proxy for sporulation intensity in the cultured macroalga Ulva (Chlorophyta)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Omri Nahor ◽  
Cristina F. Morales-Reyes ◽  
Gianmaria Califano ◽  
Thomas Wichard ◽  
Alexander Golberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Life Cycle ◽  
Abiotic Factors ◽  
Physiological Status ◽  
Seaweed Cultivation ◽  
Reproductive Growth ◽  
Biotic And Abiotic Factors ◽  
Flow Cytometric ◽  
Abiotic Stimuli ◽  
Fundamental Shift

Abstract Controlling the life cycle of the green macroalga Ulva (Chlorophyta) is essential to maintain its efficient aquaculture. A fundamental shift in cultivation occurs by transforming the thallus cells into gametangia and sporangia (sporulation), with the subsequent release of gametes and zoids. Sporulation occurrence depends on algal age and abiotic stimuli and is controlled by sporulation inhibitors. Thus, quantification of sporulation intensity is critical for identifying the biotic and abiotic factors that influence the transition to reproductive growth. Here, we propose to determine the sporulation index by measuring the number of released gametes using flow cytometry, in proportion to the total number of thallus cells present before the occurrence of the sporulation event. The flow cytometric measurements were validated by manually counting the number of released gametes. We observed a variation in the autofluorescence levels of the gametes which were released from the gametangia. High autofluorescence level correlated to phototactically active behaviour of the gametes. As autofluorescence levels varied between different groups of gametes related to their mobility, flow cytometry can also determine the physiological status of the gametes used as feedstock in seaweed cultivation.

Atomic-layer-controlled deposition of TEMAZ/O2–ZrO2 oxidation resistance inner surface coatings for solid oxide fuel cells

2016 ◽  
Vol 288 ◽  
pp. 211-220 ◽  
Author(s):  
Thomas Keuter ◽  
Georg Mauer ◽  
Frank Vondahlen ◽  
Riza Iskandar ◽  
Norbert H. Menzler ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Solid Oxide Fuel Cells ◽  
Oxidation Resistance ◽  
Atomic Layer ◽  
Solid Oxide ◽  
Surface Coatings ◽  
Oxide Fuel ◽  
Inner Surface ◽  
Controlled Deposition

Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes

2006 ◽  
Vol 111 (3) ◽  
pp. 197-205 ◽  
Author(s):  
Konstantinos Papadimitriou ◽  
Harris Pratsinis ◽  
Gerhard Nebe-von-Caron ◽  
Dimitris Kletsas ◽  
Effie Tsakalidou
Keyword(s):  
Flow Cytometry ◽  
Rapid Assessment ◽  
Fluorescence Probes ◽  
Physiological Status

Investigating the Antibacterial Effects of Plant Extracts on Pseudomonas aeruginosa and Escherichia coli

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Somayeh Jahani ◽  
Saeide Saeidi ◽  
Fereshteh Javadian ◽  
Zahra Akbarizadeh ◽  
Ali Sobhanizade
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Plant Extracts ◽  
Antibacterial Effects

Feasibility of disinfection kinetics and minimum inhibitory concentration determination on bacterial cultures using flow cytometry

2008 ◽  
Vol 58 (4) ◽  
pp. 937-944 ◽  
Author(s):  
J. H. Cunningham ◽  
C. Cunningham ◽  
B. Van Aken ◽  
L.-S. Lin
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Minimum Inhibitory Concentration ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Microbial Consortium ◽  
Silver Ion ◽  
Single Type ◽  
Physiological Status ◽  
Bacterial Cultures

Disinfection kinetics has been well established for selected antimicrobial agents on isolated bacterial strains. Due to the difficulties of culturing most bacteria, the majority of these studies have been limited to readily cultivable microorganisms of a single type or family. This study explores the feasibility of using flow cytometry for characterising the disinfection kinetics and minimum inhibitory concentration (MIC) of an Escherichia coli culture and a microbial consortium. The proposed method relies on fluorescent dye molecules to indicate the morphological and physiological status of numerous individual cells. Biocides of varying effectiveness and inactivation mechanisms (chlorine, iodine, and silver) were used to evaluate this novel application. Using pseudo-first-order kinetics, the coefficients of specific lethality of chlorine and iodine on Escherichia coli were 4.71 and 3.78×10−3 L mg−1 min−1 and MIC of silver ion was between 60 and 80 μg L−1. The coefficients of specific lethality of chlorine and iodine on the microbial consortium were 4.96 and 8.89×10−3 L mg−1 min−1 and MIC of silver ion was between 40 and 60 μg L−1. This method can be used to provide a rapid and consistent way of determining disinfection kinetics and MICs for pure and mixed bacterial cultures and can potentially be used to examine water and wastewater disinfection efficiency. However, caution should be used to ensure that the physiological and morphological status characterised by cytodyes is a result of the inactivation mechanisms of the disinfectants evaluated.

scholarly journals ANTIBACTERIAL ACTIVITY OF THREE PLANT EXTRACTS AGAINST MULTIDRUG RESISTANCE PSEUDOMONAS AERUGINOSA

2017 ◽  
Vol 10 (12) ◽  
pp. 193 ◽  
Author(s):  
Yassir Haider Almawlah ◽  
Hadeel Alaa ◽  
Sanaa O Aljelawi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Multidrug Resistance ◽  
Plant Extracts ◽  
Punica Granatum ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Active Components ◽  
Burn Wound ◽  
Test Organisms

Objective: The increasing of antibiotic resistance of Pseudomonas aeruginosa causes serious worldwide infection problems. Hence, the objective of the study was to investigate the antibacterial activity of some plants against multidrug resistance P. aeruginosa isolated from burn-wound infections.Methods: About 30 burn-wound swab samples taken from hospitalized patients in Al-Hillah Teaching Hospital, Babylon Province. P. aeruginosa isolates were isolated and identified depending on cultural, microbial, and biochemical characteristics. Then, the drug susceptibility was detected using different available antibiotics (8) to select multidrug resistance P. aeruginosa strains for using as test organisms. Three types of plants, including onion bulbs (Allium cepa), leaves of mint (Mentha asiatica), and outer peel of pomegranate (Punica granatum), were extracted by three types of solvent. The plant extracts were tested as antimicrobial substances.Results: About 9 samples were found positive by causing bacterial infection who presented invasive burn-wound infection from both sex and average age of 9 to 45 years. P. aeruginosa was found to be the most common isolates, 10 samples out of 30 samples. The most of multidrug resistance P. aeruginosa was used as test organisms to investigate the antimicrobial activity of three types of plant extracts. The plant extract of P. granatum showed the highest antibacterial activity, followed by A. cepa, and finally, M. asiatica.Conclusion: From the study, all three studied plants had antibacterial activity against multidrug-resistant P. aeruginosa isolated from burn wound. It is a recommendation that natural products can use as therapeutic agents will probably not elicit resistance in bacteria. More research must continue to isolate and purify the active components and applied in experimental animal models. 

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