Causal organism of flacherie in the silkworm Antheraea assama Ww: Isolation, characterization and its inhibition by garlic extract

2002 ◽  
Vol 16 (S1) ◽  
pp. 89-90 ◽  
Author(s):  
Arundhati Choudhury ◽  
Arijit Guha ◽  
Archana Yadav ◽  
Bala G. Unni ◽  
Monoj K. Roy
2005 ◽  
Vol 40 (2) ◽  
pp. 197-205
Author(s):  
Arundhati Choudhury ◽  
Archana Yadav ◽  
Bala G. Unni ◽  
Dipali Devi

The protease released by Pseudomonas aeruginosa strain AC-3, a causal organism of flacherie disease in Antheraea assama Westwood silkworms, was characterized and its activity against muga silkworm larvae was assessed in laboratory studies. When grown in casein broth maximum protease production occurred when the strain was cultivated for 60 h. This protease was partially purified by acetone precipitation and subjected to SDS-PAGE. Its molecular weight was approximately 35,000 da. The partially purified protease reduced larval survivability in vivo. The hemolymph protein profile revealed an apparent detrimental effect of the protease on biologically important proteins of silkworm larvae.


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
N Ide ◽  
N Morihara ◽  
L Paptheodorou ◽  
R Stirner ◽  
N Weiss

Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.


2019 ◽  
pp. 05-09

The presence study deals with powdery mildews in various cucurbits in Katsina city (Barhim Estate, Kofar Durbi, Kofar Sauri, Kofar Marusa and Low Cost), Nigeria. The finding shows that the areas infested with powdery mildew is one of the important disease of cucurbits. The Sphaerotheca fuliginea was identified to be the causal organism present on all observed cucurbits in the study. Highest frequency of disease was found in Kofar Sauri(79%) fallowed by Kofar Marusa (68%), Kofar Durbi (66%), Barhim Estate (65%) and the lowest frequency of occurrence of disease was found in Low Cost (55%).The intensity of the disease was moderate to severe in general but it was high in many fields, the area-wise variation was also noticed. On vegetables, the highest frequency of occurrence of powdery mildew disease was observed on L. cylindrica (76.4%) followed by C. moschata (60%), C. sativus (59.3%), C. vulgaris (53.9%) and lowest was found on C. melo (44.4%). The highest intensity of disease was found on C. moschata, followed by L. cylindrica, C. sativus, C. vulgaris and C. melo.


2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.


2012 ◽  
Vol 10 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Azam J. Samdani ◽  
Samreen ◽  
M. Iqbal Choudhary ◽  
Atta-ur-Rahman

Author(s):  
SHREYASHI M ◽  
SULAGNA D ◽  
SANKARI D ◽  
THIRUMURUGAN D ◽  
INFANT SANTHOSE B ◽  
...  

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3203
Author(s):  
Syed Mohammed Basheeruddin Asdaq ◽  
Obulesu Challa ◽  
Abdulhakeem S. Alamri ◽  
Walaa F. Alsanie ◽  
Majid Alhomrani ◽  
...  

This study was conducted to determine the potential interaction of aged garlic extract (AGE) with carvedilol (CAR), as well as to investigate the role of S-allyl-l-cysteine (SAC), an active constituent of AGE, in rats with isoproterenol (ISO)-induced myocardial dysfunction. At the end of three weeks of treatment with AGE (2 and 5 mL/kg) or SAC (13.1 and 32.76 mg/kg), either alone or along with CAR (10 mg/kg) in the respective groups of animals, ISO was administered subcutaneously to induce myocardial damage. Myocardial infarction (MI) diagnostic predictor enzymes, lactate dehydrogenase (LDH) and creatinine kinase (CK-MB), were measured in both serum and heart tissue homogenates (HTH). Superoxide dismutase (SOD), catalase, and thiobarbituric acid reactive species (TBARS) were estimated in HTH. When compared with other groups, the combined therapy of high doses of AGE and SAC given alone or together with CAR caused a significant decrease in serum LDH and CK-MB activities. Further, significant rise in the LDH and CK-MB activities in HTH was noticed in the combined groups of AGE and SAC with CAR. It was also observed that both doses of AGE and SAC significantly increased endogenous antioxidants in HTH. Furthermore, histopathological observations corroborated the biochemical findings. The cytoprotective potential of SAC and AGE were dose-dependent, and SAC was more potent than AGE. The protection offered by aged garlic may be attributed to SAC. Overall, the results indicated that a high dose of AGE and its constituent SAC, when combined with carvedilol, has a synergistic effect in preventing morphological and physiological changes in the myocardium during ISO-induced myocardial damage.


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